Technique validation by liquid chromatography for the determination of acyclovir in plasma

Fernández, Marcos; Sepúlveda, Jacqueline; Aránguiz, Teobaldo; Plessing, Carlos von

doi:10.1016/s1570-0232(03)00252-6

Cited by 37 publications

(44 citation statements)

References 20 publications

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“…Many of these methods are based on high-efficiency liquid chromatography (HPLC). HPLC-PDA based methodologies are already described for ACV and its impurities determination (Tzanavaras and Themelis, 2007), as well as ACV determination in urine, plasm, amniotic fluid, fetal and placental tissues (Land and Bye, 1981;Brown et al, 2002;Fernandez et al, 2003). Besides that, quantification methods were developed for ACV determination in tablets (Ghosh et al, 2012) and microparticulate drug delivery systems (Stulzer et al, 2008).…”

Section: Acyclovirmentioning

confidence: 99%

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

2018

J App Pharm Sci

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This study aimed to develop and validate a HPLC based analytical methodology for simultaneous determination of acyclovir and curcumin within microparticles. Chromatographic separation was achieved by employing a Fenomenex C18 column as stationary phase and a ternary mixture of acetonitrile, 0.1% phosphoric acid, and methanol (50:40:10) as the mobile phase. The validated method proved to be linear in the range of 0.5-30 µg.mL −1 and 0.5-20 µg.mL −1 for acyclovir and curcumin, respectively. Detection and quantification limits for acyclovir were, respectively, 83.62 ng.mL −1 and 109.52 ng.mL −1 , while for curcumin the values were 91.61 ng.mL −1 and 128.71 ng.mL −1 , what assures the methodology sensitivity. The method was also precise (1.2% RSD for acyclovir and 1.38% RSD for curcumin), besides showing recovery rates close to 100% for both of two drugs when accuracy was accessed. Minor alterations over chromatographic setup have confirmed methodology robustness. The present methodology proved to be capable of detecting and quantifying acyclovir and curcumin at polymeric microparticles in a single run, showing itself as an analytical alternative to be employed in the quality control for this dosage form.

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Section: Acyclovirmentioning

confidence: 99%

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

2018

J App Pharm Sci

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“…Some of the analytical methods have been reported for acyclovir such as luorescence [18][19][20] direct UV [21][22][23][24] and HPLC-MS [25]. In that numerous HPLC methods have been reported for estimation of acyclovir in pharmaceutical formulations has been reported [26][27][28][29][30][31][32][33][34]. Among chromatographic techniques; the reversed-phase (RP) HPLC was widely used for the analysis.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of LC-MS/MS Method for the Analysis of P-Toluenesulfonicacid, In Antiviral Drug Acyclovir

Reddy¹

2015

IJIRSET

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A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC/MS/MS) method was developed and validated for the trace analysis (>1 ppm level) of p-Toluenesulfonicacid, a genotoxic impurity, in acyclovir drug substances. The chromatographic separation was achieved on Symmetry C-18 (150 X 4.6mm, 3.5µm) column using a mobile phase consisting of 5 mM ammonium acetate and methanol (70:30 v/v) at flow rate of 0.4 mL /min. The API-4000 LC/MS/MS was operated on an electrospray in negative mode. The selected ion monitoring (SIM) mode was used during the analytical run and it is able to quantitate up to 1.5 ppm of p-Toluenesulfonicacid. The newly developed method was validated as per achieved on International Conference on Harmonization (ICH) guidelines

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“…ACV causes suicide inactivation when the terminated DNA template containing ACV binds the viral DNA polymerase leading to its irreversible inactivation [3][4][5] . Literature surveys have reported some HPLC and immunoassay techniques, specctrofluorometry and UV-spectrophotometric techniques for the analytical estimation of ACV 1,[6][7][8][9] . Some of the spectrophotometric methods are based on oxidative-coupling reactions using mostly ceric ammonium sulphate or potassium persulfate as oxidizing reagents followed by coupling with 3-methylbenzothiazoline 2-one hydrazone (MBTH reagent) to form colored chromogens detectable at 630 nm.…”

Section: Introductionmentioning

confidence: 99%

Uv-Spectrophotometric Estimation of Acyclovir in Bulk and Pharmaceutical Dosage Forms

Padala¹,

Baishakhi²,

Assaleh³

et al. 2013

J Pharm Sci Innov

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Analytical method development being a vital part of pre formulation-formulation research and development obviates the need to develop reliable, effective, eco friendly and cost effective methodologies for routine analysis of active pharmaceutical ingredients. UV spectroscopy is one of the earliest, yet of wide applications in drug analysis in different stages of formulations and quality control; despite the availabilities of sophisticated chromatographic techniques and other hyphenated techniques. Current research attempts to develop simple, sensitive, accurate, precise and economical UV spectrophotometric methods for the routine analysis of acyclovir in bulk and pharmaceutical dosage forms using two separate alkaline media, 0.1N NaOH (method A) and 0.1N KOH (method B) and validate them as per ICH guidelines. In both the methods maximum absorbance was observed at 264 nm. Beer's law was obeyed in the concentration of 2.5-40 µg / mL in method A and 2.5-30 µg / mL in method B with correlation coefficient of 0.999. The % recovery carried out by adding known amount of standard drug to pre-analyzed tablet solutions was 98.75 ± 0.52 % to 99.78 ± 0.69 % (method A) and 98.55 ± 0.31 % to 99.78 ± 0.22 % (method B). Intra and interday precision expressed in % RSD were 0.38 ± 0.01 and 0.27 ± 0.02 -0.44 ± 0.01 respectively and the percent purity was 99.85 ± 0.05 %. The methods were validated statistically as per ICH guidelines and the results obtained were within the acceptance criteria for the parameters relating to linearity, accuracy, precision.

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Technique validation by liquid chromatography for the determination of acyclovir in plasma

Cited by 37 publications

References 20 publications

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

Development and Validation of LC-MS/MS Method for the Analysis of P-Toluenesulfonicacid, In Antiviral Drug Acyclovir

Uv-Spectrophotometric Estimation of Acyclovir in Bulk and Pharmaceutical Dosage Forms

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