Technique in the Study of Yeast Assimilation Reactions

Barnett, James A.; Ingram, M.

doi:10.1111/j.1365-2672.1955.tb02070.x

Cited by 62 publications

(22 citation statements)

References 19 publications

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“…Cultures were grown in a mineral salts medium containing I ml trace elements solution (Barnett & Ingram, 1955)/1, plus (g/l): K2HP04, 0.5; NH,CI, 1.0; MgS04.7H20, 0.02; yeast extract (Oxoid), 0.2; Casamino acids (Difco), 0 . 1 ; aromatic carbon source, 0.5; the pH was adjusted to 7-2.…”

Section: E T H O D Smentioning

confidence: 99%

“…The degradation of aromatic compounds by mesophilic micro-organisms (reviewed by Dagley, 1971) and the oxidative breakdown of phenolic compounds by several genera of aerobic bacteria and yeasts is well documented (Bayly, Dagley & Gibson, 1966;Feist & Hegeman, 1969;Neujahr & Varga, 1970;Neujahr, Lindsjo & Varga, 1974). However, there is little information concerning the utilization of aromatics by thermophiles.…”

Section: Introductionmentioning

confidence: 99%

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Utilization of Phenol and Cresols Bacillus stearothermophilus, Strain PH24

Buswell

Twomey

1975

Journal of General Microbiology

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Section: E T H O D Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Utilization of Phenol and Cresols Bacillus stearothermophilus, Strain PH24

Buswell

Twomey

1975

Journal of General Microbiology

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“…for the culture of adapted cells consisted of (g./l. ): KH2PO4, 0-1; K2HPO4, 0-4; (NH4)2504, 1-0; MgS04,7H20, 0-01; Oxoid yeast extract, 0-1; aromatic substrate, 1-5; traceelements solution (Barnett & Ingram, 1955), 10m1./l. ; made up in tap water.…”

mentioning

confidence: 99%

Bacterial attack on phenolic ethers: An enzyme system demethylating vanillic acid

Cartwright

Smith

1967

Biochemical Journal

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1. A cell-free system from Pseudononaw fluorescens catalysed the oxidative demethylation and subsequent ring-cleavage of vanillate, with uptake of 2-5 moles of oxygen/mole of substrate. 2. Demethylation involved absorption of 0-5mole of oxygen/mole, and required reduced glutathione (GSH) and nucleotide (probably NADPH) as cofactors, with further possible requirements, the natures of which are discussed. 3. Incomplete evidence suggested that the aromatic ring was opened via protocatechuate and the appropriate oxygenase, with absorption of 1mole of oxygen/mole of substrate, eventually yielding ,-oxoadipate. 4. The methyl group was removed sequentially as formaldehyde, formate and carbon dioxide, the steps catalysed respectively by formaldehyde dehydrogenase, which required GSH and NAD+, and formate dehydrogenase. Each enzyme was cytochrome-linked and accounted for absorption of 0-5 mole of oxygen/mole of substrate. 5. All enzymes except formate dehydrogenase, which was a cell-wall enzyme, resided in the soluble fraction of the extract. The demethylase could not be resolved because of unknown cofactor requirements.The interpretation of the results of bacterial attack on lignin is difficult because of the chemical complexity of this material. To obtain essential background information for this difficult problem we have examined the bacterial degradation of substances that could be products of lignin catabolism or that, by dehydrogenation and condensation reactions, could be converted into lignin in plants.We now present, for the first time, observations on demethylation processes by partly resolved bacterial enzymes. Whereas Axelrod (1956) and Brodie et al. (1955) demonstrated salient features of the enzymic demethylation of methoxyphenyl derivatives in mammalian tissues, no detailed information has been reported on the process in micro-organisms; although, for example, Henderson (1957Henderson ( , 1961 showed that demethylated products arise from the attack by fungal cells on methoxybenzoic acids.

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“…The media for testing the utilization of the carbon sources and of KN03 were those used by Wickerham (1951) except that the amino acids were omitted (Barnett & Ingram, 1955) and 50 mwcarbon sources were added Yeast ident$cation on silica gel media 581 (Buhagiar & Barnett, 1971). In the medium containing KNO, as the sole nitrogen source, the concentration of KH,PO, was increased to 5 g 1-l.…”

Section: Removdl Of D-glucose and D-fructosefrom The Sugar Solutionsmentioning

confidence: 99%

The Use of a Colloidal Silica Preparation in Media Used for Yeast Identification

Buhagiar

1977

Journal of General Microbiology

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Technique in the Study of Yeast Assimilation Reactions

Cited by 62 publications

References 19 publications

Utilization of Phenol and Cresols Bacillus stearothermophilus, Strain PH24

Utilization of Phenol and Cresols Bacillus stearothermophilus, Strain PH24

Bacterial attack on phenolic ethers: An enzyme system demethylating vanillic acid

The Use of a Colloidal Silica Preparation in Media Used for Yeast Identification

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