Technical manual potato reproductive and cytological biology

Ordoñez, B.; Orrillo, M.; Bonierbale, Merideth

doi:10.4160/9789290604808

Cited by 3 publications

(2 citation statements)

References 12 publications

(13 reference statements)

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“…For NCM, anti-A or/and anti-B antibodies were titrated after incubating the samples and diluents at 37°C for 30 min, using a reported method [ 8 ] that was modified to enhance the sensitization of SAS and ABO antibodies, simulating in vivo conditions. Anti-A and/or anti-B antibody titers were measured using gel column agglutination with dedicated centrifuges and incubators, following the manufacturer’s instructions (Grifols, Barcelona, Spain).…”

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confidence: 99%

Comparative Analysis of AB vs. ABO-specific Plasma for Desensitization in Blood Group O Recipients: An In Vitro Study

Lim

2024

Ann Lab Med

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Neutralizing capacity measurement (NCM) of soluble ABH substances (SAS) in plasma was assessed to guide the selection of the appropriate ABO group of fresh-frozen plasma (FFP) for plasma exchange (PE) in blood group O recipients with ABO-incompatible transplantations. Neutralizing capacity was assessed by measuring anti-A and/or anti-B titers in samples comprising one unit of O FFP and 10 O EDTA plasma samples and subtracting the binary logarithm of the titer in each group with a saline dilution. Ten EDTA plasma samples with Lewis b (Le b ) antigen positivity and 10 sets of pooled FFP from each blood group were used as diluents. In O FFP, the NCM values (mean±SD) were 3.4±0.52 (2.6±0.52) and 2.6±0.52 (1.5±0.3) in B and AB for IgM (total antibody) anti-B (both P <0.001), and in the 10 O EDTA plasma samples, they were 3.9±0.88 (3.1±0.88) and 3.2±0.79 (2.4±0.97) for IgM ( P =0.0013) and total anti-B ( P =0.025), respectively. In vitro analysis revealed that B FFP is more effective than AB FFP in reducing IgM and total anti-B antibody titers in O recipients, regardless of Le b antigen positivity.

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confidence: 99%

Comparative Analysis of AB vs. ABO-specific Plasma for Desensitization in Blood Group O Recipients: An In Vitro Study

Lim

2024

Ann Lab Med

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“…-Análisis de viabilidad del polen mediante tinción con carmín acético: se preparan unos granos de polen en un portaobjetos, y sobre ellos se añaden unas gotas de carmín acético preparado según el protocolo estándar (González et al, 2002;Ordóñez, 2014;Ordóñez, Orrillo and Bonierbale, 2017). Esta preparación se protege con un cubreobjetos y en el microscopio (200 X) se evalúa el número de granos teñidos (viables) con relación al total, estimando el grado de viabilidad de ese polen.…”

Section: Cruzamientos Y Semillas: Diseño De Cruzamientos Dialélicosunclassified

Selección de parentales para obtención acelerada de variedades de patata (Solanum tuberosum L.) con aptitud para procesamiento industrial mediante la aplicación de un modelo genético dialélico

Rivero¹

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With the aim of evaluating several advanced breeding clones and varieties of potato (S. tuberosum) for their ability to transmit traits of interest to their progenies, a diallelic assay has been developed for this tetraploid and vegetatively propagated species, with six parents crossed in a diallel group, Griffing's model I, method 4, since the reciprocals were not included. A set of progenies was obtained and tested at various locations for 3 years. Data from field trials focused on agronomic traits, plant maturity and yield were collected, and tuber quality analysis were performed, focused on the specific gravity of the tubers and chip color.

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Comparison of Column Agglutination Technique and Tube Test for ABO Antibody Titration and Crossmatching

Lim

2023

Korean J Blood Transfus

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Background: ABO Ab titration using Erytra Eflexis (Diagnostic Grifols, Barcelona, Spain, abbreviated as Eflexis) and a pretransfusion crossmatching using DG Gel Coombs Card (Diagnostic Grifols, abbreviated as Card) were compared with the tube test (TT) to help laboratories. Methods: Samples subjected to TT were titrated for ABO antibody using the Eflexis. The titers obtained were thereafter converted into log steps of 2 (n=20 for each ABO group). The reference range (RR) of the ABO Ab titer using Eflexis was determined using samples from health checkup patients (n=30 for each ABO group). The Card and LISS-antihuman globulin (AHG) test of TT were compared for 40 cases with identified antibodies. Results: The average titers of IgM anti-B of blood group A by TT (3.2 vs 2.4, P＜0.001), and anti-A of type O by Eflexis (4.5. vs 5.5, P=0.001 for IgM, 6.3. vs 7.3, P＜0.001 for total antibody) were higher compared to the alternative method. The RRs of IgM (total antibody) of anti-B and anti-A were determined to be 1:2≤ (1:2≤) for anti-B in group A, 1:8≤ (1:8≤) for anti-A in group B, and 1:8≤ (1:32≤) and 1:16≤ (1:32≤) for anti-B and anti-A in group O, respectively. A comparison of crossmatching revealed that the average hemagglutination strength of the Card was 1.65, which was stronger than the LISS-AHG of 0.50 (P＜0.001). Conclusion:The results of this study are expected to provide valuable insights for the establishment and guidance of laboratory tests utilizing Eflexis and Card.

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Technical manual potato reproductive and cytological biology

Cited by 3 publications

References 12 publications

Comparative Analysis of AB vs. ABO-specific Plasma for Desensitization in Blood Group O Recipients: An In Vitro Study

Comparative Analysis of AB vs. ABO-specific Plasma for Desensitization in Blood Group O Recipients: An In Vitro Study

Selección de parentales para obtención acelerada de variedades de patata (Solanum tuberosum L.) con aptitud para procesamiento industrial mediante la aplicación de un modelo genético dialélico

Comparison of Column Agglutination Technique and Tube Test for ABO Antibody Titration and Crossmatching

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