Technical evaluation of serological screening tests for anti-Toxoplasma gondii antibodies to prevent unnecessary transfusion risks

Vaz, Rogério Saad; Guimarães, Ana Tereza Bittencourt; Bonanato, Larissa D.; Soccol, Vanete Thomaz

doi:10.1590/s1516-84842008000400009

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…This is a cross-sectional study regarding the seroprevalence and molecular evaluation of T. gondii infection among healthy blood donors of Boyer-Ahmad County in southwest Iran. The overall seroprevalence of 16.8% for toxoplasmosis in the current study indicates a considerable rate of toxoplasmosis in the population, but the seroprevalence rate of toxoplasmosis in our study is lower than the rates reported in blood donors from most of the areas in Iran [ 2 , 7 , 25 ] and other regions as reported from Turkey (22.59%) [ 26 ], Czech Republic (34.23%) [ 27 ], Brazil (60%) [ 28 ], Saudi Arabia (40%) [ 29 ], Iraq (32.75%) [ 30 ], Egypt (59.6%) [ 31 ], and India (53.7%) [ 32 ]. Variations in the rate of seropositivity of T. gondii in different regions of the world or in different areas of a given country can be attributed to the differences in climate, topographical conditions, and food behavior.…”

Section: Discussioncontrasting

confidence: 71%

Toxoplasma gondiiin Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran

Moshfe

Arefkhah

Sarkari

et al. 2018

Interdisciplinary Perspectives on Infectious Diseases

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Toxoplasma gondii is an important foodborne protozoan that can be transmitted through infected blood containing tachyzoite form of the parasite. The current study aimed to evaluate the prevalence of T. gondii infection and related risk factors among healthy blood donors in Boyer-Ahmad County, southwest Iran. Blood samples were taken from 285 healthy blood donors who voluntarily agreed to participate in this study. Sera and buffy coat were isolated from the blood samples for serological and molecular evaluations. The sera were tested for anti-T. gondii antibodies (both IgG and IgM), using a commercial ELISA kit. The buffy coat of seropositive cases was evaluated for detection of T. gondii DNA by PCR. Moreover, a structured questionnaire, containing socioepidemiological data and possible risk factors, was filled out by each participant during sample collection. Anti-T. gondii antibodies were detected in sera of 48/285 (16.8%) participants. Only two of the subjects (0.7%) were seropositive for both IgG and IgM antibodies. T. gondii DNA was not detected in buffy coat of any of the seropositive cases. Risk factors such as contact with soil (OR, 9.7; 95% CI, 4.9–19.4) and consumption of semicooked meat (OR, 2.5; 95% CI, 1.2–5.03) were statistically associated with seropositivity to T. gondii. The seroprevalence rate of T. gondii antibodies in the blood donors of Boyer-Ahmad County was not high in comparison with other regions in Iran. In this study, consumption of undercooked meats, job, and contact with soil were independent risk factors associated with T. gondii infection, which can be considered as potential sources of T. gondii infection.

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Section: Discussioncontrasting

confidence: 71%

Toxoplasma gondiiin Blood Donors: A Study in Boyer-Ahmad County, Southwest Iran

Moshfe

Arefkhah

Sarkari

et al. 2018

Interdisciplinary Perspectives on Infectious Diseases

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“…Screening for anti- T. gondii in donated blood is not compulsory in many countries, including Brazil, but studies have shown the importance of this transmission route [18, 19]. Despite the inherent risk, there are many questions about the effectiveness of implementing testing to identify toxoplasmosis [20].…”

Section: Introductionmentioning

confidence: 99%

“…Siegel et al [ 17 ] described one of the first instances of T. gondii transmission by the transfusion of blood products in which four immunocompromised individuals developed toxoplasmosis. Screening for anti- T. gondii in donated blood is not compulsory in many countries, including Brazil, but studies have shown the importance of this transmission route [ 18 , 19 ]. Despite the inherent risk, there are many questions about the effectiveness of implementing testing to identify toxoplasmosis [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: the first report in the Americas

et al. 2017

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BackgroundIn Brazil, there have been no previous studies of Toxoplasma gondii infection in sickle cell anemia patients and carriers of severe forms of beta-thalassemia. This study evaluated T. gondii infection in patients with beta-hemoglobinopathies.MethodsA total of 158 samples, 77 (48.7%) men and 81 (51.3%) women, were evaluated. Three groups were formed: G1 (85 patients with sickle cell disease); G2 (11 patients with homozygous beta-thalassemia; G3 (62 patients with heterozygous beta-thalassemia). ELISA was employed to identify anti-T. gondii IgM and IgG antibodies, and molecular analysis was performed to determine beta-hemoglobin mutations. Fisher’s exact test was used to compare frequencies of anti-T. gondii IgM and IgG antibodies in respect to gender and age.ResultsAnti-T. gondii IgG antibodies were found in 43.5% of individuals in G1, 18.1% in G2 and 50% in G3. All samples from G1 and G2 were seronegative for anti-T. gondii IgM antibodies, but 3.2% from G3 were seropositive. Considering anti-T. gondii IgG antibodies, no statistical significant differences were found between these groups nor in seroprevalence between genders within each group. Despite this, comparisons of the mean ages between G1, G2 and G3 were statistically significant (G2 vs. G1: p value = 0.0001; G3 vs. G1: p-value <0.0001; G3 vs. G2: p-value = 0.0001).ConclusionA comparison by age of patients with sickle cell anemia showed a trend of lower risk of infection among younger individuals. Therefore, this study demonstrates that T. gondii infection occurs in patients with beta-thalassemia and sickle cell anemia in Brazil as seen by the presence of anti-T. gondii IgM and IgG antibodies.

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