Technical and clinical comparison of two fully automated methods for the immunoassay of CA 125 in serum

Yan, Guifeng; Ju, Huangxian; Zhi-Chao, Liang; Ting-Qing, Zhang

doi:10.1016/s0022-1759(99)00023-x

Cited by 18 publications

(7 citation statements)

References 8 publications

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“…Additionally, the fluctuation of CA125 levels in healthy women is inconsistent with the assumptions that underlie traditional linear regression analysis. Researchers in 16 previous studies that evaluated CA125 fluctuations using multiple assays used linear statistical procedures that require normal Gaussian distributions (Bon et al, 1995(Bon et al, , 1999Bonfrer, Baan, Jansen, Lentfer, & Kenemans, 1994;Bonfrer et al, 1997;Clement et al, 1995;Davelaar et al, 1998Davelaar et al, , 2003Fisken et al, 1989;Kafali et al, 2007;Kenemans, van Kamp, Oehr, & Verstraeten, 1993;Kenemans, Verstraeten, van Kamp, & Mensdorff-Pouilly, 1995;Lehtovirta et al, 1990;Martin & Blockx, 1997;van Kamp, Verststraeten, & Kenemans, 1993;Yan, Ju, Liang, & Zhang, 1999;Yedema, Thomas, Segers, Doesburg, & Kenemans, 1992). The present study is the first to use more conservative statistical procedures that account for wide interindividual differences in CA125 levels.…”

mentioning

confidence: 88%

A Comparison of the Cyclic Variation in Serum Levels of CA125 Across the Menstrual Cycle Using Two Commercial Assays

McLemore

Aouizerat

Lee

et al. 2011

Biological Research For Nursing

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The two commercial assays for CA125 determination demonstrated good concordance in terms of reference ranges regardless of epitope differences. While CA125 levels changed over the course of the menstrual cycle, these changes may not be clinically significant in healthy women. This study is the first to control for factors known to contribute to CA125 elevations; to quantify a decrease in CA125 levels across the menstrual cycle; and to confirm concordance in the relative decreases in serum CA125 levels across the menstrual cycle between two frequently used commercial assays.

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mentioning

confidence: 88%

A Comparison of the Cyclic Variation in Serum Levels of CA125 Across the Menstrual Cycle Using Two Commercial Assays

McLemore

Aouizerat

Lee

et al. 2011

Biological Research For Nursing

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“…CA125 levels in human sera have been widely used in monitoring patients with ovarian cancer, and will become a useful indicator for tumor regression, relapse, and prognosis. So far, CA125 levels were usually measured by immunoradiometric assay [31] or enzyme IA [32]. However, these methods were time-consuming, labor-extensive, and required high reagent amounts.…”

Section: Introductionmentioning

confidence: 99%

A sensitive and rapid immunoassay for quantification of CA125 in human sera by capillary electrophoresis with enhanced chemiluminescence detection

Wang

Ren

2005

Electrophoresis

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In this paper we have presented a sensitive and rapid immunoassay (IA) method by capillary electrophoresis with an enhanced chemiluminescence detection system (CE-CL) based on the catalytic effects of horseradish peroxidase (HRP) on the luminol-hydrogen peroxide reaction. The conditions for the CL reaction and electrophoresis were systematically investigated using HRP as a model sample. The linear range from 2.5 x 10(-11) to 1.0 x 10(-9) mol/L (R = 0.999), and the detection limit of 1.0 x 10(-12) mol/L (signal-to-noise ratio = 3) for HRP were achieved using para-iodophenol as CL enhancer. The relative standard deviations of the migration time and peak area for 5.0 x 10(-10) mol/L HRP (n = 7) were 0.26 and 4.8%, respectively, using a CE system with a home-built CL detector. Under the optimal condition, the HRP-labeled CA125 antibody (Ab) and the Ab-antigen complex were well separated within 4 min by CE using a high-pH buffer (pH 10.20). The assay was successfully used for quantification of CA125 in human sera from health controls and patients associated with ovarian cancer, and the recoveries of the standard addition experiments were 93-109%. Our primary results demonstrated that IA based on CE-CL detection is a powerful tool for clinical diagnosis combined with these commercial IA kits.

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“…This method has been widely used in mineral analysis in rocks, materials analysis, environmental monitoring , and in the detection of antibiotics and other contaminants in foods . CLIA has also been widely used in the clinical detection of tumor markers, such as alpha‐fetoprotein (AFP), prostate‐specific antigen, CA125, and human chorionic gonadotrophin (hCG) , as well as for detection of diabetes, thyroid, and reproductive hormones . However, because the mainstream products of chemiluminescent reagents and instruments use proprietary technologies, these platforms are not conducive to the common alterations and development within the scientific community.…”

Section: Introductionmentioning

confidence: 99%

The Establishment of an HE4‐CLIA Method and the Combined Analysis of HE4 and CA125 in Ovarian Cancer

Zhang

Wang

et al. 2016

Clinical Laboratory Analysis

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Technical and clinical comparison of two fully automated methods for the immunoassay of CA 125 in serum

Cited by 18 publications

References 8 publications

A Comparison of the Cyclic Variation in Serum Levels of CA125 Across the Menstrual Cycle Using Two Commercial Assays

A Comparison of the Cyclic Variation in Serum Levels of CA125 Across the Menstrual Cycle Using Two Commercial Assays

A sensitive and rapid immunoassay for quantification of CA125 in human sera by capillary electrophoresis with enhanced chemiluminescence detection

The Establishment of an HE4‐CLIA Method and the Combined Analysis of HE4 and CA125 in Ovarian Cancer

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