Technetium-99m-labeled annexin V imaging for detecting prosthetic joint infection in a rabbit model

Tang, Cheng; Wang, Feng; Hou, Yuemei; Lu, Shanshan; Tian, Wei; Xu, Yan; Jin, Chang Hyun; Wang, Liming

doi:10.7555/jbr.29.20130113

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…With the hypothesis that radiolabeled Annexin-V selectively binds to apoptotic PMNs, Annexin-V was labeled with 99m Tc and was successfully evaluated to image experimental abscesses in animals and infection in humans. 187–190…”

Section: Annexin-vmentioning

confidence: 99%

Currently Available Radiopharmaceuticals for Imaging Infection and the Holy Grail

Salmanoglu

Kim

Thakur

2018

Seminars in Nuclear Medicine

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Infection is ubiquitous. However, its management is challenging for both the patients and the health-care providers. Scintigraphic imaging of infection dates back nearly half a century. The advances in our understanding of the pathophysiology of disease at cellular and molecular levels have paved the way to the development of a large number of radiopharmaceuticals for scintigraphic imaging of infection. These include radiolabeling of blood elements such as serum proteins, white blood cells (WBCs), and cytokines, to name a few. Infectious foci have also been imaged using a radiolabeled sugar molecule by taking advantage of increased metabolic activity in the infectious lesions. Literature over the years has well documented that none of the radiopharmaceuticals and associated procedures that facilitate imaging infection are flawless and acceptable without a compromise. As a result, only a few compounds such as Tc-hexamethylpropyleneamineoxime,F-FDG, the oldest but still considered as a gold standard In-oxine, and, yes, evenGa-citrate in some countries, have remained in routine clinical practice. Nonetheless, the interest of scientists and physicians to improve the approaches to imaging and to the management of infection is noteworthy. These approaches have paved the way for the development of numerous, innovative radiopharmaceuticals to label autologous WBCs ex vivo or even those that could be injected directly to image infection or inflammation without direct involvement of WBCs. In this review, we briefly describe these agents with their pros and cons and place them together for future reference.

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Section: Annexin-vmentioning

confidence: 99%

Currently Available Radiopharmaceuticals for Imaging Infection and the Holy Grail

Salmanoglu

Kim

Thakur

2018

Seminars in Nuclear Medicine

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“…Technetium-99m annexin A5 was described by Ohtsuki et al in 1999 for the non-invasive visualization of thymic apoptosis in rodents [ 69 ]. Dual radio- and fluorescently-labelled annexin A5 was utilized with in vivo single-photon emission computed tomography (SPECT) imaging of rodent models to investigate the macrophage infiltration of atherosclerotic plaques [ 70 , 71 ], tumor apoptosis visualization [ 72 ], prosthetic joint infection [ 73 ] and stroke [ 74 ] (although the latter is considered to be somewhat controversial [ 75 ]). More recently, the synthesis of annexin A5 functionalized nanoparticles has been described to permit multimodal imaging in rodents with computed tomography imaging of atherosclerotic plaques [ 76 ].…”

Section: In Vivo Apoptosis Quantification With Annexin A5mentioning

confidence: 99%

Annexins in Glaucoma

Yap

Davis²,

Guo³

et al. 2018

IJMS

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Glaucoma is one of the leading causes of irreversible visual loss, which has been estimated to affect 3.5% of those over 40 years old and projected to affect a total of 112 million people by 2040. Such a dramatic increase in affected patients demonstrates the need for continual improvement in the way we diagnose and treat this condition. Annexin A5 is a 36 kDa protein that is ubiquitously expressed in humans and is studied as an indicator of apoptosis in several fields. This molecule has a high calcium-dependent affinity for phosphatidylserine, a cell membrane phospholipid externalized to the outer cell membrane in early apoptosis. The DARC (Detection of Apoptosing Retinal Cells) project uses fluorescently-labelled annexin A5 to assess glaucomatous degeneration, the inherent process of which is the apoptosis of retinal ganglion cells. Furthermore, this project has conducted investigation of the retinal apoptosis in the neurodegenerative conditions of the eye and brain. In this present study, we summarized the use of annexin A5 as a marker of apoptosis in the eye. We also relayed the progress of the DARC project, developing real-time imaging of retinal ganglion cell apoptosis in vivo from the experimental models of disease and identifying mechanisms underlying neurodegeneration and its treatments, which has been applied to the first human clinical trials. DARC has potential as a biomarker in neurodegeneration, especially in the research of novel treatments, and could be a useful tool for the diagnosis and monitoring of glaucoma.

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Conditional Tissue-Specific Foxa2 Ablation in Mouse Pancreas Causes Hyperinsulinemic Hypoglycemia: (RETRACTED)

Fei²,

Liu³

et al. 2016

American Journal of Therapeutics

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The forkhead/winged helix transcription factor Foxa2 is a major upstream regulator of Pdx1, a transcription factor necessary for pancreatic development. In the present study, we conditionally knocked out Foxa2 in Pdx1-expressing domain and further analyzed the contribution of Foxa2 to α- and β-cell development and the effect of Foxa2 deletion on plasma insulin, glucagon, and glucose levels. Homozygous pdx1 Foxa2 mice and heterozygous pdx1 Foxa2 mice were generated by homologous recombination using a Foxa2 gene-targeting vector. α- and β-cell mass was examined by immunofluorescence microscopy. Plasma glucose, insulin, and plasma were measured at postnatal day 10. For pdx1 lineage tracing studies, heterozygous pdx1 Foxa2 EYFP and homozygous pdx1 Foxa2 EYFP mice were used. Our immunofluorescence analysis revealed that in the pancreas sections of the homozygous mutant mice, Foxa2 was virtually absent from non-β cells and its expression almost exclusively coincided with remnant β cells. The density of both α and β cells apparently decreased in the pancreas of the heterozygous mutant mice and in the pancreas of the homozygous mutant mice, α cells lost its predominance and β cells increased proportionally. Direct Pdx1 cell lineage tracing revealed that, on embryonic day 18.5, in the homozygous mutant mice, Pdx1 expression coincided almost exclusively with that of insulin-secreting β cells. Chemiluminescence assays revealed that heterozygous pdx1 Foxa2 mice had significantly lower insulin levels than control mice (P < 0.01). However, no apparent difference was observed between homozygous pdx1 Foxa2 mice and control mice (P > 0.05). Chemiluminescence assays also showed that Foxa2 deletion significantly depressed plasma glucagon levels in both homozygous pdx1 Foxa2 mice and heterozygous pdx1 Foxa2 mice (P < 0.01 vs. controls). Plasma glucose on postnatal day 10 was significantly lower in homozygous pdx1 Foxa2 mice compared with control mice (P < 0.01). Our study demonstrates that homozygous Foxa2 ablation leads to an imbalance in β/α ratio, profound hypoglucagonemia, inappropriate hyperinsulinemia, and hypoglycemia in mice. Our conditional tissue-specific Foxa2 ablation mouse model will be useful in elucidating regulation of normal and abnormal α- and β-cell differentiation and pinpointing novel targets for diabetes control.

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Technetium-99m-labeled annexin V imaging for detecting prosthetic joint infection in a rabbit model

Cited by 6 publications

References 28 publications

Currently Available Radiopharmaceuticals for Imaging Infection and the Holy Grail

Currently Available Radiopharmaceuticals for Imaging Infection and the Holy Grail

Annexins in Glaucoma

Conditional Tissue-Specific Foxa2 Ablation in Mouse Pancreas Causes Hyperinsulinemic Hypoglycemia: (RETRACTED)

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