Tebufenozide resistance is associated with sex‐linked inheritance in Plutella xylostella

Abstract

The diamondback moth (DBM), Plutella xylostella (L.), is a major pest of cruciferous crops. Tebufenozide, a novel nonsteroidal ecdysone agonist, exhibits good efficacy and has played an increasingly important role in the control of Lepidopteran pests in China. For its resistance management, the genetic basis of tebufenozide resistance was studied using a laboratory selected resistant strain of DBM (resistant ratio, RR = 268). A series of crosses with laboratory susceptible and resistant strains revealed that tebufenozide resistance in this pest was partially biased toward female heredity, with a large difference in RR for F1 (RR = 29) and rF1 progeny (RR = 147). The dominance calculated for these 2 cross progeny was -0.788 and 0.09, respectively. Further analysis showed that the susceptible male and female larvae were similar in their sensitivity to tebufenozide, but the resistant female larvae showed significantly higher resistance than the resistant male larvae. The heredity of tebufenozide resistance in DBM might be linked with the W sex chromosome, which suggested that DBM has the ability to develop high levels of resistance to tebufenozide. This is the first report of sex-linked inheritance of tebufenozide resistance in P. xylostella (L.).

Characterization of UDP‐glucuronosyltransferase genes and their possible roles in multi‐insecticide resistance in Plutella xylostella (L.)

“…The results showed that the BL population had developed high resistance to chlorantraniliprole, phoxim, spinosyns, chlorfenapyr, metaflumizone, abamectin and indoxacarb (180-1285-fold) and medium resistance to beta-cypermethrin (11-fold) and tebufenozide (43-fold), while still being susceptible to Cry1Ac (0.45-fold) (Table 1). The resistance of P. xylostella to the 10 insecticides tested here has been reported elsewhere; 28,[46][47][48][49][50] resistance to only a few insecticides was found in one population. However, here, a typical multi-resistant population was identified that showed medium to high resistance to at least nine insecticides with different modes of action.…”

Monitoring resistance of Phenacoccus solenopsis Tinsley (Homoptera: Pseudococcidae) to new chemical insecticides in Punjab, Pakistan

“…Previously, resistance to insect growth regulators have been reported in a variety of insect pests, e.g. B. tabaci (Crowder et al, 2008;Ma et al, 2010), M. domestica (Bell et al, 2010;Tang et al, 2002), P. xylostella (Cao and Han, 2013;Sun et al, 2012) and S. litura Rehan and Freed, 2014;Shad et al, 2012). The variability in resistance could be due to different selection pressure on each population and different resistance mechanisms among the populations resulting in either a delay or an increased rate of resistance development (Silva et al, 2011).…”

In Vitro Binding Effects of the Ecdysone Receptor−Binding Domain and PonA in Plutella xylostella

“…In insects, the ecdysone receptor that regulates the metamorphosis process is a nuclear receptor. The commercialized insecticide tebufenozide kills some lepidopteran and coleopteran pests by interfering with the normal molting process [ 4 ].…”