Tebuconazole induced oxidative stress related hepatotoxicity in adult and larval zebrafish (Danio rerio)

Li, Shuying; Jiang, Yao; Sun, Qianqian; Coffin, Scott; Chen, Lili; Qiao, Kun; Gui, Wenjun; Zhu, Guonian

doi:10.1016/j.chemosphere.2019.125129

Cited by 66 publications

(19 citation statements)

References 39 publications

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“…These enzymes are also important in examining the health status of fish. Thus, decreased activity of this enzyme is a reason for proper liver function and suitable nutritional conditions [20]. In our present study, the result indicated that the highest level of liver enzyme AST was observed in the Fig.…”

Section: Enzyme Analysissupporting

confidence: 60%

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Concurrent metformin and silibinin therapy in diabetes: assessments in zebrafish (Danio rerio) animal model

Mohammadi

Manouchehri

Changizi

et al. 2020

J Diabetes Metab Disord

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Objective In this study, zebrafish was used as a biological model to induce type 2 diabetes mellitus through glucose. Then, the effect of metformin and silibinin combination was examined on elevated blood glucose, intestinal tissues, liver enzymes, and TNF-α, IFN-γ, INL1β genes as inflammation marker genes. Methods The liver enzymes (AST, ALT, and ALP) derived from fish viscera homogenate supernatants were assayed in an auto-analyzer. The expression of target genes was quantified on RNA extracted from the tails by an in-house RT-PCR method, with fine intestine tissue staining performed by hematoxylin and eosin protocol (H&E). Result In the glucose-free treatments, metformin and silymarin decreased the levels of AST, ALT, and ALP enzymes in the blood. The combination of these two drugs had also a significant role in reducing glucose levels. The body weight increased significantly in the control group which was affected by glucose concentration, with the lowest body weight gain observed in the metformin group. The expression of INL-1β gene was significantly enhanced in the control group and the highest IFN-γ expression was observed in both control groups with glucose (G + CTRL) and without glucose (G-CTRL) (p < 0.05). The lowest level of TNF-α gene expression was observed in the control + glucose group (G + CTRL) (p < 0.05). Diabetic state causes weak absorption whereby the fish body demands to increase absorption level by enhancing the amount of acidic goblet cells thereby acidifying the environment in the gastric tracts. Conclusion Collectively, this study indicated that treatment with metformin and Silibinin could improve metabolic-mediated performances by reducing the expression of inflammatory genes and blood glucose, modulating liver enzymes, and ameliorating the intestinal inflammation in type 2 diabetic zebrafish model.

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Section: Enzyme Analysissupporting

confidence: 60%

“…The samples were then centrifuged in a microtube at 2500 rpm (4°C) for 15 min. After centrifugation, the soluble supernatant was used to measure the enzymes: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkannin phosphatase (ALP) [20].…”

Section: Blood Glucose Measurement and Enzyme Determinationsmentioning

confidence: 99%

Concurrent metformin and silibinin therapy in diabetes: assessments in zebrafish (Danio rerio) animal model

Mohammadi

Manouchehri

Changizi

et al. 2020

J Diabetes Metab Disord

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“…The correlation and PCA analysis have also indicated that tebuconazole presence may reduce longevity and egg viability in C. elegans. This fungicide toxicity in reproduction has already been reported in different animal models (Guimaraes et al 2018;Li et al 2020;Machado-Neves et al 2018;Fig. 5 Correlation analysis of all data, independent on samples collected pre-or post-pesticide application Fig.…”

Section: Discussionmentioning

confidence: 67%

“…Tebuconazole is a fungicide that has been frequently detected in agriculture systems at concentrations that affect endocrine function in organisms. For instance, it has been found to cause oxidative stress and endocrine disruption in rats (Yang et al 2018) and developmental toxicity associated to thyroid dysfunction in zebrafish (Li et al 2020). Finally, imazethapyr has also been reported by causing metabolic alterations in Cyprinus carpio (Moraes et al 2011), genotoxicity in tadpoles of Rhinella arenarum and Hypsiboas pulchellus species (Carvalho et al 2019;Perez-Iglesias et al 2017;Perez-Iglesias et al 2015), and also in mammalian cells (Soloneski et al 2017); however, the toxicity of herbicide is poorly characterized and needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Kuhn

Jacques

Teixeira

et al. 2021

Environ Sci Pollut Res

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Uruguay River is the most important river in western Rio Grande do Sul, separating Brazil from Argentina and Uruguay. However, its pollution is of great concern due to agricultural activities in the region and the extensive use of pesticides. In a long term, this practice leads to environmental pollution, especially to the aquatic system. The objective of this study was to analyze the physicochemical characteristics, metals and pesticides levels in water samples obtained before and after the planting and pesticides' application season from three sites: Uruguay River and two minor affluents, Mezomo Dam and Salso Stream. For biomonitoring, the free-living nematode Caenorhabditis elegans was used, which were exposed for 24 h. We did not find any significant alteration in physicochemical parameters. In the pre-and post-pesticides' samples we observed a residual presence of three pesticides (tebuconazole, imazethapyr, and clomazone) and metals which levels were above the recommended (As, Hg, Fe, and Mn). Exposure to both pre-and post-pesticides' samples impaired C. elegans reproduction and post-pesticides samples reduced worms' survival rate and lifespan. PCA analysis indicated that the presence of metals and pesticides are important variables that impacted C. elegans biological endpoints. Our data demonstrates that Uruguay River and two affluents are contaminated independent whether before or after pesticides' application season. In addition, it reinforces the usefulness of biological indicators, since simple physicochemical analyses are not sufficient to attest water quality and ecological safety.

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“…[39] GSH levels expressed as chromatographic peak areas of the GSH-NEM conjugate significantly decreased in TEB group and increased in CELE9 treated groups in a dose-dependent manner (Figure 6D). Recent studies have suggested that TEB induces oxidative stress in the livers of fish [38,40] and mammals, [41] leading to the production of free radicals. Our data revealed that CELE9 had protective effects against TEB-induced liver injury, which could be attributed to the in vivo antioxidant and glutathione-repleting activity of CELE9.…”

Section: Hepatoprotection Against the Agricultural Fungicide Tebucona...mentioning

confidence: 99%

Chemical Constituents of Cyperus esculentus Leaves and the Protective Effect against Agricultural Fungicide‐Induced Hepatotoxicity

Meng

et al. 2022

Chemistry & Biodiversity

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Cyperus esculentus is cultivated as a crop plant due to its edible and oily tubers (tiger nut). However, little is known about the phytochemicals and bioeffects of the leaves. This study was conducted to identify and quantify the chemical constituents of C. esculentus leaves and evaluate their bioactivities. By liquid chromatography‐mass spectrometry, 30 compounds including flavan‐3‐ols, caffeic acid derivatives, and flavones, were identified from the leaves. The quantitative analysis revealed that gallocatechin (8), procyanidin B1 (15), catechin (16), chlorogenic acid (19), orientin (30), and luteolin 7‐O‐glucuronide (31) are the major chemical constituents of C. esculentus leaves. The contents of these six chemical constituents in the leaves collected in September in Hohhot, China, reached to 1460.85±7.66, 10178.77±302.65, 1048.35±17.37, 1722.15±26.13, 5318.62±277.16, and 1526.54±11.95 μg, respectively, in one gram of the dried leaves. The leaf extract (CELE) showed strong antioxidant activity in vitro, with compounds 8, 15, and 19 contributing the most. CELE showed significant protection against the agricultural fungicide tebuconazole‐induced developmental toxicity and hepatotoxicity in zebrafish.

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Tebuconazole induced oxidative stress related hepatotoxicity in adult and larval zebrafish (Danio rerio)

Cited by 66 publications

References 39 publications

Concurrent metformin and silibinin therapy in diabetes: assessments in zebrafish (Danio rerio) animal model

Concurrent metformin and silibinin therapy in diabetes: assessments in zebrafish (Danio rerio) animal model

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Chemical Constituents of Cyperus esculentus Leaves and the Protective Effect against Agricultural Fungicide‐Induced Hepatotoxicity

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