“…routine histological evaluation of plastic-or resinembedded, toluidine blue-stained sections (mainly used for the PNS; Greaves 2007); cytoskeletal labeling of CNS or PNS axons by histological stains (e.g., silver impregnation [Bielschowsky's, Bodian's]) or immunohistochemistry (e.g., anti-NFP); teased fiber preparations, applicable to PNS axons and myelin (Krinke, Vidotto, and Weber 2000a). Differential diagnoses: axonal dystrophy (see definition in this glossary) Comment: The term axonal degeneration is the preferred term for this change as it is the most general form.…”
Harmonization of diagnostic nomenclature used in the pathology analysis of tissues from rodent toxicity studies will enhance the comparability and consistency of data sets from different laboratories worldwide. The INHAND Project (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) is a joint initiative of four major societies of toxicologic pathology to develop a globally recognized nomenclature for proliferative and nonproliferative lesions in rodents. This article recommends standardized terms for classifying changes observed in tissues of the mouse and rat central (CNS) and peripheral (PNS) nervous systems. Sources of material include academic, government, and industrial histopathology databases from around the world. Covered lesions include frequent, spontaneous, and aging-related changes as well as principal toxicant-induced findings. Common artifacts that might be confused with genuine lesions are also illustrated. The neural nomenclature presented in this document is also available electronically on the Internet at the goRENI website (http://www.goreni.org/).
“…routine histological evaluation of plastic-or resinembedded, toluidine blue-stained sections (mainly used for the PNS; Greaves 2007); cytoskeletal labeling of CNS or PNS axons by histological stains (e.g., silver impregnation [Bielschowsky's, Bodian's]) or immunohistochemistry (e.g., anti-NFP); teased fiber preparations, applicable to PNS axons and myelin (Krinke, Vidotto, and Weber 2000a). Differential diagnoses: axonal dystrophy (see definition in this glossary) Comment: The term axonal degeneration is the preferred term for this change as it is the most general form.…”
Harmonization of diagnostic nomenclature used in the pathology analysis of tissues from rodent toxicity studies will enhance the comparability and consistency of data sets from different laboratories worldwide. The INHAND Project (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) is a joint initiative of four major societies of toxicologic pathology to develop a globally recognized nomenclature for proliferative and nonproliferative lesions in rodents. This article recommends standardized terms for classifying changes observed in tissues of the mouse and rat central (CNS) and peripheral (PNS) nervous systems. Sources of material include academic, government, and industrial histopathology databases from around the world. Covered lesions include frequent, spontaneous, and aging-related changes as well as principal toxicant-induced findings. Common artifacts that might be confused with genuine lesions are also illustrated. The neural nomenclature presented in this document is also available electronically on the Internet at the goRENI website (http://www.goreni.org/).
“…We prepared single fibers from teased sciatic nerves (see Materials and Methods) (Krinke et al, 2000) and performed immunostaining with antibodies raised against Pan voltage-gated sodium channel (VGSC) or antibodies raised against paranodin/ contactin-associated protein (Caspr). We did not observe alterations in the distributions of VGSC or paranodin/Caspr in the sciatic nerves of the FAD-linked PS1⌬E9 rotorod mice compared with the wild-type mice exposed to the rotorod (Fig.…”
Section: Morphological and Electrophysiological Parameters Are Unaffementioning
Presenilins (PS) play a central role in ␥-secretase-mediated processing of -amyloid precursor protein (APP) and numerous type I transmembrane proteins. Expression of mutant PS1 variants causes familial forms of Alzheimer's disease (FAD). In cultured mammalian cells that express FAD-linked PS1 variants, the intracellular trafficking of several type 1 membrane proteins is altered. We now report that the anterograde fast axonal transport (FAT) of APP and Trk receptors is impaired in the sciatic nerves of transgenic mice expressing two independent FAD-linked PS1 variants. Furthermore, FAD-linked PS1 mice exhibit a significant increase in phosphorylation of the cytoskeletal proteins tau and neurofilaments in the spinal cord. Reductions in FAT and phosphorylation abnormalities correlated with motor neuron functional deficits. Together, our data suggests that defects in anterograde FAT may underlie FAD-linked PS1-mediated neurodegeneration through a mechanism involving impairments in neurotrophin signaling and synaptic dysfunction.
“…The stained specimens are transferred to glycerin, where, using a pair of fine forceps and a stereomicroscope, they are separated into smaller fibre bundles from which single fibres are isolated. The slides are then mounted in glycerin-gelatine and examined under a light microscope (Krinke et al, 2000).…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.