Tear Fluid Protein Biomarkers

You, Jungmok; Willcox, Mark; Madigan, Michele C.; Wasinger, Valerie C.; Schiller, Belinda; Walsh, Bradley J.; Graham, Peter; Kearsley, John H.; Li, Yong

doi:10.1016/b978-0-12-800096-0.00004-4

Cited by 45 publications

(56 citation statements)

References 165 publications

(193 reference statements)

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“…Body fluid biomarkers have the advantages of less invasive collection methods and thus a better acceptance by patients or animal owners. 104 Despite ease of blood sampling, identifying potential biomarkers in plasma or serum poses difficulties because of the high sample complexity, low concentrations of individual markers, and degradation of biomarkers. 10,16,57 Analysis of more proximal samples (eg, tears or aqueous humor) may present higher sensitivity and specificity for screening and monitoring glaucoma-related biomarkers.…”

Section: Future Workmentioning

confidence: 99%

Genetic and Biochemical Biomarkers in Canine Glaucoma

2016

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In many health-related fields, there is great interest in the identification of biomarkers that distinguish diseased from healthy individuals. In addition to identifying the diseased state, biomarkers have potential use in predicting disease risk, monitoring disease progression, evaluating treatment efficacy, and informing pathogenesis. This review details the genetic and biochemical markers associated with canine primary glaucoma. While there are numerous molecular markers (biochemical and genetic) associated with glaucoma in dogs, there is no ideal biomarker that allows early diagnosis and/or identification of disease progression. Genetic mutations associated with canine glaucoma include those affecting ADAMTS10, ADAMTS17, Myocilin, Nebulin, COL1A2, RAB22A, and SRBD1. With the exception of Myocilin, there is very limited crossover in genetic biomarkers identified between human and canine glaucomas. Mutations associated with canine glaucoma vary between and within canine breeds, and gene discoveries therefore have limited overall effects as a screening tool in the general canine population. Biochemical markers of glaucoma include indicators of inflammation, oxidative stress, serum autoantibodies, matrix metalloproteinases, tumor necrosis factor-α, and transforming growth factor-β. These markers include those that indicate an adaptive or protective response, as well as those that reflect the damage arising from oxidative stress.

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Section: Future Workmentioning

confidence: 99%

Genetic and Biochemical Biomarkers in Canine Glaucoma

2016

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“…Behcet's disease (BD) is a chronic, relapsing systemic inflammatory disease characterized by recurrent oral aphthae and a variety of other clinical features, including uveitis (also known as intraocular inflammation), skin lesions, gastrointestinal involvement, neurologic disease, vascular disease and arthritis [10] . Half of BD patients may associate with uveitis, which is a common cause of vision loss and blindness worldwide, especially in young men [11] . Interestingly, despite being a symmetrical organ, the eyes of patients with Bechet's disease uveitis (BDU) with unilateral uveitis relapse show dramatically distinct clinical manifestations.…”

Section: Introductionmentioning

confidence: 99%

Unilateral relapse of Behcet’s disease-associated uveitis does not appear to cause asymmetric tear protein profiles

Liang

Qin

Zhao

et al. 2018

Preprint

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Statement of clinical relevanceTears are an easily, noninvasively accessible body fluid that is a valuable source of biomarkers for various diseases. Behcet's disease uveitis (BDU) has high potential to cause blindness and represents the leading cause of morbidity in BD patients, especially in frequently relapsing cases. Here, we adopted a method combining a "dry" method for tear preservation and nano-LC-DIA-MS/MS system to explore whether unilateral relapse of BDU causes differences in the tear proteome between the diseased and the contralateral quiescent eye, with the aim of evaluating tear fluid as a source for biomarker studies of uveitis relapse. AbstractPurpose: To explore whether unilateral relapse of Bechet's disease uveitis (BDU) causes differences in the tear proteome between the diseased and the contralateral quiescent eye. Experimental design: To minimize interindividual variations, bilateral tear samples were collected from the same patient (n=15) with unilateral relapse of BDU. A data-independent acquisition (DIA) strategy was used to identify proteins that differed between active and quiescent eyes. Results: A total of 1,797 confident proteins were identified in the tear samples, of which 371 are also highly expressed in various tissues and organs. Sixty-two (3.5%) proteins differed in terms of expression between tears in active and quiescent eyes, similar to the number of differentially expressed proteins (74, 4.1%) identified in a randomized grouping strategy. Furthermore, the intrapair trend of the differentially expressed proteins was not consistent and none of the proteins showed the same trend in more than 9 pairs of eyes. Conclusions and clinical relevance: Unilateral relapse of BDU does not appear to cause asymmetric changes in the tear proteome between active and contralateral quiescent eyes. Tear fluid is a valuable source for biomarker studies of systemic diseases.

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“…12Y14 One of the biggest advantages of developing contact lens sensors is it provides a noninvasive alternative to current invasive methods to monitor diseases. 15,16 This overview will highlight the major progress to date in developing contact lens sensors for continuous, semicontinuous, and noncontinuous detection of diseases and several of the key technical and clinical challenges in this field as reported in the peer-reviewed literature. Because of space considerations, although we have extensively discussed peer-reviewed publications, it has not been possible to take into consideration the entire patent literature in this area, which is extensive.…”

mentioning

confidence: 99%

The Use of Contact Lenses as Biosensors

Phan

Subbaraman

Jones

2016

Optometry and Vision Science

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: The tear film is a complex multilayer film consisting of various proteins, enzymes, and lipids and can express a number of biomarkers in cases of disease. The development of a contact lens sensor presents a noninvasive alternative for the detection and management of various diseases. Recent work has resulted in the commercialization of a device to monitor intraocular pressure for up to 24 h, and there are extensive efforts underway to develop a contact lens sensor capable of continuous glucose tear film monitoring to manage diabetes. This clinical perspective will highlight the major developments within this field and list some of the major challenges that still need to be addressed.

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Tear Fluid Protein Biomarkers

Cited by 45 publications

References 165 publications

Genetic and Biochemical Biomarkers in Canine Glaucoma

Genetic and Biochemical Biomarkers in Canine Glaucoma

Unilateral relapse of Behcet’s disease-associated uveitis does not appear to cause asymmetric tear protein profiles

The Use of Contact Lenses as Biosensors

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