Tear film inflammatory cytokine upregulation in contact lens discomfort

Gad, Anne; Vingrys, Algis J.; Wong, Christine; Jackson, David C.; Downie, Laura E

doi:10.1016/j.jtos.2018.10.004

Cited by 32 publications

(23 citation statements)

References 58 publications

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“…As reported in a series of clinical studies, higher IL-17a in tear were detected in patients with contact lens discomfort and more severe dry eye related symptoms. 12,29 Besides, it was demonstrated that increased IL-17a level was detected in the DED cases with diabetes comparing with the DED cases without diabetes and it highlighted the effect of IL-17a caused by the systemic inflammation on the DED incidence. 12 In this current experiment, we provided evidences demonstrating that increased hyperosmolarity would induce up-regulation of IL-17a in the CEC and then lead to the activation of macrophage.…”

Section: Discussionmentioning

confidence: 92%

Nicotinamide Mononucleotide Alleviates Hyperosmolarity-Induced IL-17a Secretion and Macrophage Activation in Corneal Epithelial Cells/Macrophage Co-Culture System

Yu¹,

Pu²,

Dai³

et al. 2021

JIR

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Background Hyperosmosis stress (HS) was a key pathological factor in the development of dry eye disease (DED). Nicotinamide mononucleotide (NMN) demonstrated protective effects in the corneal damage, however, its role in the HS-induced DED remained unclear. Methods A NaCl based HS in-vitro model (500 mOsm) was generated and used in a co-culture system including corneal epithelial cells (CEC) and macrophage cell line RAW264.7. The effect of NMN on NAD+ metabolism and the expression of HS biomarker, tonicity-responsive element binding protein (TonEBP), was studied in the CEC. The cellular activity, including cell viability, apoptosis status and lactate dehydrogenase (LDH) release through trypan blue staining, flow cytometry and LDH assay, respectively. The mitochondrial membrane potential (MMP) assay would be conducted using the JC1 kit. The expression of IL-17a were detected using RT-PCR, ELISA and Western blot. After co-culture with the CEC in different group for 24 h, the phagocytosis ability and macrophage polarization were assessed in RAW264.7 cells co-cultured with CEC with or without HS or NMN treatment. Besides, the involvement of Notch pathway in the RAW264.7 would be analyzed. The potential involvement of Sirtuin 1 (SIRT1) and IL-17a in the crosstalk between CEC and macrophage was studied with SIRT1 inhibitor EX 527 and anti-IL-17a monoclonal antibody, respectively. Results NMN treatment increased NAD+ concentration and thus improved cell viability, reduced apoptotic rate and decreased the LDH release in HS-treated CEC. Besides, NMN alleviated HS-induced MMP, intracellular ROS and LDH release. Besides, it was confirmed NMN improve SIRT1 function and decreased the HS related IL-17a expression in CEC and then alleviated macrophage phagocytosis ability and M1 polarization based on a CEC-macrophage co-culture system. Moreover, NMN treatment of CEC in the CEC could moderate the subsequent macrophage activation through Notch pathway. SIRT1 activation and IL-17a inhibition was regarded as key progress in the function of NMN based on the application of EX 527 and anti-IL-17a antibody in the CEC-macrophage co-culture system. Conclusion The findings demonstrated that NMN could alleviated HS-induced DED status through regulating the CEC/macrophage interaction. Our data pointed to the role of SIRT1, IL-17a and Notch pathway in the function of NMN and then provided updated knowledge of potential NMN application in the management of DED.

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Section: Discussionmentioning

confidence: 92%

Nicotinamide Mononucleotide Alleviates Hyperosmolarity-Induced IL-17a Secretion and Macrophage Activation in Corneal Epithelial Cells/Macrophage Co-Culture System

Yu¹,

Pu²,

Dai³

et al. 2021

JIR

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“…Participants underwent comprehensive ocular surface health examinations using our previously established protocols [13]. Primary outcome measures were dry eye symptoms measured using the validated Ocular Surface Disease Index (OSDI) questionnaire [14], tear film osmolarity measured using the Tearlab system (Tearlab Corp., San Diego, CA, USA), and extent of meibomian gland dropout measured by meibography.…”

Section: Methodsmentioning

confidence: 99%

Meibomian gland dropout is associated with immunodeficiency at HIV diagnosis: Implications for dry eye disease

et al. 2020

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Aim: To characterize anterior eye health and tear film characteristics in individuals with human immunodeficiency virus (HIV) undergoing anti-retroviral therapy. Methods:This cross-sectional study involved 35 adults, categorized as healthy controls (n=18) or as HIV-positive patients (n=17), with no history of opportunistic infection or current ocular fundus abnormalities. Participants underwent a comprehensive anterior eye assessment. Primary outcome measures were dry eye symptoms (Ocular Surface Disease Index survey), tear film osmolarity, and extent of meibomian gland dropout. Secondary outcomes measures were ocular redness, tear film stability, and ocular surface staining.Levels of 36 cytokines were assayed from basal tears using a multiplex bead array. Results:The HIV-positive group showed more extensive meibomian gland dropout relative to controls (mean ± SD, controls: 29.6 ± 5.8 versus 37.0 ± 13.9%, p=0.045). The extent of meibomian gland dropout was negatively correlated with blood CD4 T-cell count (a marker of immunodeficiency) at diagnosis (r= -0.69, p=0.006). All other tests of anterior ocular health, including dry eye symptom levels, were not significantly different between the groups. There were no significant inter-group differences for the 36 cytokines assayed in the tear film. Conclusions:We find greater meibomian gland dropout in HIV-positive individuals that is related to disease severity at diagnosis. Given this feature predisposes to dry eye disease, it suggests the need for long-term studies of anterior eye health in people with HIV.

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“…Conversely, other studies have shown no association between non-invasive TBUT, tear volume (phenol red thread test) and comfort in subjects fitted with SiHy contact lenses [407], or when comparing dropouts to successful contact lens wearers [431]. Tear film osmolality findings are also inconclusive, given no associations with comfort were identified in short-term or dispensing studies [406,407], but increased tear film osmolality [387], and a greater difference between inferior and superior tear osmolality [432] were associated with symptoms amongst habitual contact lens wearers. With regards to tear film osmolality, these findings seem to suggest that long term studies of habitual wearers where tear film changes have had the opportunity to equilibrate may be more meaningful than short-term studies or studies evaluating previous contact lens wear dropouts.…”

Section: Patient-related Risk Factorsmentioning

confidence: 97%

“…Inflammatory mediators in the tear film including leukotriene B4 [433,434], prolactin-induced protein [433,434], prolactin-induced protein [435], cytokines [436] and interleukin 17A [432] have been associated with decreased comfort during contact lens wear, although other studies have reported no association between tear cytokines [437], arachidonic acid mediators and histamine concentrations [438] and comfort. The ratio of certain tear film components such as wax esters to triacylglycerols is different between symptomatic and asymptomatic lens wearers [428].…”

Section: Patient-related Risk Factorsmentioning

confidence: 99%

BCLA CLEAR - Contact lens complications

Stapleton

Bakkar

Carnt

et al. 2021

Contact Lens and Anterior Eye

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Contact lens-related complications are common, affecting around one third of wearers, although most are mild and easily managed. Contact lenses have well-defined anatomical and physiological effects on the ocular surface and can result in other consequences due to the presence of a biologically active material. A contact lens interacts with the tear film, ocular surface, skin, endogenous and environmental microorganisms, components of care solutions and other antigens which may result in disease specific to contact lens wear, such as metabolic or hypersensitivity disorders. Contact lens wear may also modify the epidemiology or pathophysiology of recognised conditions, such as papillary conjunctivitis or microbial keratitis. Wearers may also present with intercurrent disease, meaning concomitant or pre-existing conditions unrelated to contact lens wear, such as allergic eye disease or blepharitis, which may complicate the diagnosis and management of contact lens-related disease.Complications can be grouped into corneal infection (microbial keratitis), corneal inflammation (sterile keratitis), metabolic conditions (epithelial: microcysts, vacuoles, bullae, tight lens syndrome, epithelial oedema; stromal: superficial and deep neovascularisation, stromal oedema [striae/folds], endothelial: blebs, polymegethism/ pleomorphism), mechanical (corneal abrasion, corneal erosion, lens binding, warpage/refractive error changes; superior epithelial arcuate lesion, mucin balls, conjunctival epithelial flaps, ptosis, discomfort), toxic and allergic disorders (papillary conjunctivitis, solution-induced corneal staining, incomplete neutralisation of peroxide, Limbal Stem Cell Deficiency), tear resurfacing disorders/dry eye (contact lens-induced dry eye, Meibomian gland dysfunction, lid wiper epitheliopathy, lid parallel conjunctival folds, inferior closure stain, 3 and 9 o'clock stain, dellen, dimple veil) or contact lens discomfort. This report summarises the best available evidence for the classification, epidemiology, pathophysiology, management and prevention of contact lensrelated complications in addition to presenting strategies for optimising contact lens wear.

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Tear film inflammatory cytokine upregulation in contact lens discomfort

Cited by 32 publications

References 58 publications

Nicotinamide Mononucleotide Alleviates Hyperosmolarity-Induced IL-17a Secretion and Macrophage Activation in Corneal Epithelial Cells/Macrophage Co-Culture System

Nicotinamide Mononucleotide Alleviates Hyperosmolarity-Induced IL-17a Secretion and Macrophage Activation in Corneal Epithelial Cells/Macrophage Co-Culture System

Meibomian gland dropout is associated with immunodeficiency at HIV diagnosis: Implications for dry eye disease

BCLA CLEAR - Contact lens complications

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