Teamed boronate affinity-functionalized branched polyethyleneimine-modified magnetic nanoparticles for the selective capture of ginsenosides from rat plasma

Zhao, Ningning; Liu, Zhiqiang; Xing, Junpeng; Zheng, Zhong; Song, Fengrui; Liu, Shu

doi:10.1016/j.cej.2019.123079

Cited by 30 publications

(10 citation statements)

References 51 publications

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“…The selective extraction involves first the pH-responsive formation of reversible covalent interaction between sugars and boronic acid ligands. ,− As shown in Figure , increasing the extraction pH to a more alkaline environment could partly enhance the binding efficiency, as nonactive boronic acids (sp 2 ) can be efficiently converted into their reactive form (sp 3 ) at pH > p K a of PBAs. This in turn facilitates the formation of PBA–sugar complexes through an intermolecular hydrogen bond. , The strength and the stability of the formed boronate esters depend much on the their structures and the steric factors .…”

Section: Resultsmentioning

confidence: 99%

Highly Efficient Production and Simultaneous Purification of Lactulose via Isomerization of Lactose through an Innovative Sustainable Anion-Extraction Process

Wang

et al. 2020

ACS Sustainable Chem. Eng.

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The traditional chemical isomerization of lactose into lactulose faces unfavorable thermodynamic limitations, significant side reactions, and high purification cost challenges. Herein, an efficient strategy for combining extraction-assisted isomerization and simultaneous purification of lactulose is presented. Under optimized conditions, 4-vinylphenylboronic acid (VPBA) favored the selective extraction of lactulose and up to 80% of lactulose can be extracted with a high selectivity (>95%). The extracted lactulose can be rapidly (<10 min) back-extracted into an acidic HCl solution as a concentrated lactulose stream (∼20 g/L). Lactulose purity can significantly be improved to 99.64% through a two-staged stripping process as a function of HCl contents. In situ removal of the newly formed lactulose catalyzed by Na 2 HPO 4 + NaOH through selective extraction could significantly promote lactulose yield and minimize the formation of byproducts. The applied extraction-assisted isomerization strategy enabled the yield of lactulose up to ∼70% (batch-extraction model) and ∼85% (in situ extraction model), with the lowest yield of byproducts (<5%) ever found. Remarkably, the VPBA-dominated organic phase and the catalyst-containing aqueous phase could be easily regenerated and reused multiple times, well proving their feasibility in clean and sustainable industrial applications. This trial validates for the first time the use of the extraction-assisted strategy for an effective simultaneous isomerization and isolation of high-purity lactulose.

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Section: Resultsmentioning

confidence: 99%

Highly Efficient Production and Simultaneous Purification of Lactulose via Isomerization of Lactose through an Innovative Sustainable Anion-Extraction Process

Wang

et al. 2020

ACS Sustainable Chem. Eng.

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“…As another strategy commonly employed in the synthesis of boronate affinity sorbents, phenylboronate ligands are immobilized on functionalized support materials via organic linkers. Different supports like core-shell magnetic structures [111][112][113], alginate [114], silica [110,115], zirconia [116], graphene oxide [117], and methacrylate-based polymer [118] with different porous properties have been used in the preparation of boronate affinity sorbents with this strategy. The PBA ligands that have been widely used in post modifica-tion of support materials are APBA and VPBA.…”

Section: Boronate Affinity Chromatographymentioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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“…Besides, more boronic acids moieties derived from the branched scaffold of PEI will not only leads to larger binding capacity but also stronger affinity toward the saponin. [37][38][39][40][41] Moreover, because of the high affinity of the p-BA-MNPs toward the saponin target, the existence of competing cis-diol compounds such as monosaccharides will not interfere the evolution, ensuring efficient screening. Benet from these, ssDNA that bind with the target with weak affinity could be washed away in each round of evolution.…”

Section: Introductionmentioning

confidence: 99%

PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient in vitro evolution of saponin-binding aptamers

Zhang

Huang

et al. 2021

RSC Adv.

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Teamed boronate affinity-functionalized branched polyethyleneimine-modified magnetic nanoparticles for the selective capture of ginsenosides from rat plasma

Cited by 30 publications

References 51 publications

Highly Efficient Production and Simultaneous Purification of Lactulose via Isomerization of Lactose through an Innovative Sustainable Anion-Extraction Process

Highly Efficient Production and Simultaneous Purification of Lactulose via Isomerization of Lactose through an Innovative Sustainable Anion-Extraction Process

Recent trends in sorbents for bioaffinity chromatography

PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient in vitro evolution of saponin-binding aptamers

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