“…Water extraction [ 26 , 27 ], organic solvent extraction [ 4 , 28 , 29 , 30 ], mixed solvent extraction [ 31 ], microwave-assisted extraction [ 5 , 32 , 33 ], ultrasonic-assisted extraction [ 34 , 35 , 36 ], supercritical extraction [ 37 ], aqueous enzymatic [ 38 , 39 , 40 ] and fermentation [ 41 ] are methods utilized for extracting saponins from tea-seed saponins. Although alcohol extraction, especially ethanol extraction, appears to bear a higher yield in tea-seed extractions [ 15 ], due to the difference of extraction objects, for instance, seed meals of C. oleifera for tea-seed saponins and leaves of C. sinensis for tea-leaf saponins, water extraction might be a better choice for extracting tea-leaf saponins, as inferred and discussed by Yu. and He.…”