Tea polyphenols ameliorate hydrogen peroxide- and constant darkness-triggered oxidative stress via modulating the Keap1/Nrf2 transcriptional signaling pathway in HepG2 cells and mice liver

Qi, Guoyuan; Mi, Yashi; Fan, Rong; Li, Runnan; Wang, Yiwen; Li, Xingyu; Huang, Shuxian; Liu, Xuebo

doi:10.1039/c7ra05000c

Cited by 32 publications

(26 citation statements)

References 59 publications

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“…Moreover, as shown in Figure 2, our results also demonstrated that the overexpression of HO-1 in Hep3B cells attenuated H 2 O 2 -induced ROS generation. These results supported recent studies, suggesting that the upregulation of HO-1 alleviated H 2 O 2 -induced ROS and oxidative injury in HepG2 cells [49,50], although, in this study, we did not determine whether ectopic HO-1 overexpression inhibited the apoptosis of hepatoma cells. Since a recent study indicated that IL-6 drives ROS reduction by increasing Nrf 2, a transcription activator of the HO-1 gene, expression in human islet β-cells [51], further investigations into the role of IL-6-HO-1 crosstalk in hepatoma cells, in terms of ROS synthesis, are warranted.…”

Section: Discussionsupporting

confidence: 84%

Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells

et al. 2020

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Heme oxygenase-1 (HO-1) has several important roles in hepatocytes in terms of anti-inflammation, anti-apoptosis, and antioxidant properties. Interleukin-6 (IL-6) is a pleiotropic cytokine associated with liver regeneration and protection against injury. The aim of this study was to determine the potential crosstalk between HO-1 and IL-6, and to elucidate the signaling pathways involved in the induction of HO-1 by IL-6 in human hepatoma cells. Ectopic overexpression of HO-1 not only attenuated cell proliferation in vitro and in vivo, but also blocked the reactive oxygen species (ROS) induced by H2O2 and the pyocyanin in HepG2 or Hep3B cells. IL-6 expression was negatively regulated by HO-1, while IL-6 induced signal transducer and activator of transcription 3 (STAT3) phosphorylation and HO-1 gene expression in HepG2 cells. The co-transfected HO-1 reporter vector and a protein inhibitor of the activated STAT3 (PIAS3) expression vector blocked the IL-6-induced HO-1 reporter activity. Both interferon γ and interleukin-1β treatments induced STAT1 but not STAT3 phosphorylation, which had no effects on the HO-1 expression. Treatments of AG490 and luteolin blocked the JAK/STAT3 signaling pathways which attenuated IL-6 activation on the HO-1 expression. Our results indicated that HO-1 is the antitumor gene induced by IL-6 through the IL-6/JAK/STAT3 pathways; moreover, a feedback circuit may exist between IL-6 and HO-1 in hepatoma cells.

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Section: Discussionsupporting

confidence: 84%

Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells

et al. 2020

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“…However, pretreatment with different concentrations of PHP could signicantly (P < 0.05) up-regulated the gene expressions of these antioxidant enzymes variously in an obvious concentration-dependent manner, which was similar to the previous reports on antioxidants. 15,34 Particularly, compared with the control group, the cells treated with 1.00 and 2.00 mg mL À1 PHP showed signicant increase in mRNA levels of CAT, which was consistent with the activity of CAT, indicating that PHP had the similar concentration-dependent manner effects on the gene expression of CAT (Fig. 2D).…”

Section: Gene Expression Of Antioxidant Enzymes Nrf2 and Keap1supporting

confidence: 65%

Cytoprotective effects of a tripeptide from Chinese Baijiu against AAPH-induced oxidative stress in HepG2 cells via Nrf2 signaling

Sun

Luo³

et al. 2018

RSC Adv.

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“…This action would be facilitated by increased binding activity of NF-κB DNA ( 163 ), a transcription factor that is activated by ROS and regulates the inflammatory and immune response ( 165 ). Moreover, Qi et al ( 176 ) recently found that the apoptosis triggered by DD extends to the liver due to the oxidative damage caused by the overproduction of ROS after a marked reduction in the mRNA levels of antioxidant enzymes.…”

Section: Constant Darknessmentioning

confidence: 99%

Dim Light at Night and Constant Darkness: Two Frequently Used Lighting Conditions That Jeopardize the Health and Well-being of Laboratory Rodents

González¹

2018

Front. Neurol.

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The influence of light on mammalian physiology and behavior is due to the entrainment of circadian rhythms complemented with a direct modulation of light that would be unlikely an outcome of circadian system. In mammals, physiological and behavioral circadian rhythms are regulated by the suprachiasmatic nucleus (SCN) of the hypothalamus. This central control allows organisms to predict and anticipate environmental change, as well as to coordinate different rhythmic modalities within an individual. In adult mammals, direct retinal projections to the SCN are responsible for resetting and synchronizing physiological and behavioral rhythms to the light-dark (LD) cycle. Apart from its circadian effects, light also has direct effects on certain biological functions in such a way that the participation of the SCN would not be fundamental for this network. The objective of this review is to increase awareness, within the scientific community and commercial providers, of the fact that laboratory rodents can experience a number of adverse health and welfare outcomes attributed to commonly-used lighting conditions in animal facilities during routine husbandry and scientific procedures, widely considered as “environmentally friendly.” There is increasing evidence that exposure to dim light at night, as well as chronic constant darkness, challenges mammalian physiology and behavior resulting in disrupted circadian rhythms, neural death, a depressive-behavioral phenotype, cognitive impairment, and the deregulation of metabolic, physiological, and synaptic plasticity in both the short and long terms. The normal development and good health of laboratory rodents requires cyclical light entrainment, adapted to the solar cycle of day and night, with null light at night and safe illuminating qualities during the day. We therefore recommend increased awareness of the limited information available with regards to lighting conditions, and therefore that lighting protocols must be taken into consideration when designing experiments and duly highlighted in scientific papers. This practice will help to ensure the welfare of laboratory animals and increase the likelihood of producing reliable and reproducible results.

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Tea polyphenols ameliorate hydrogen peroxide- and constant darkness-triggered oxidative stress via modulating the Keap1/Nrf2 transcriptional signaling pathway in HepG2 cells and mice liver

Abstract: Tea polyphenols alleviate oxidative stressviamodulating the Keap1/Nrf2 transcriptional signaling pathway in HepG2 cells and the liver of mice kept in constant darkness.

Cited by 32 publications

References 59 publications

Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells

Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells

Cytoprotective effects of a tripeptide from Chinese Baijiu against AAPH-induced oxidative stress in HepG2 cells via Nrf2 signaling

Dim Light at Night and Constant Darkness: Two Frequently Used Lighting Conditions That Jeopardize the Health and Well-being of Laboratory Rodents

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