TDZ-Induced Morphogenesis Pathways in Woody Plant Culture

Novikova, Tatyana

doi:10.1007/978-981-10-8004-3_3

Cited by 16 publications

(9 citation statements)

References 125 publications

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“…TDZ, a cytokinin-like compound, promotes a diverse array of morphogenic responses including shoot bud proliferation [30,31]. Apart from its cytokinin-like activity, TDZ plays important role in modulation of endogenous hormone levels especially auxin/cytokinin ratio [32]. In the present study, our results revealed that the effect of KIN and TDZ combination on multiple shoot proliferation is more compared to KIN and TDZ when used separately.…”

Section: Resultssupporting

confidence: 47%

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

Soni

Keswani

Bhatt

et al. 2021

Heliyon

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An efficient and reproducible protocol for in vitro propagation of Dolichandra unguis-cati has been established for the first time from nodal segments. In order to enhance survival rate under ex vitro conditions, photosynthetic potential of in vitro grown plantlets was also studied through JIP test based analysis of polyphasic OJIP chlorophyll a fluorescence OJIP transients, density of active reaction centers, light harvesting efficiency, electron transfer rate, dissipation energy, maximum quantum yield of primary PSII photochemistry and photosynthetic performance index. The best morphogenetic in term of explants response (92.2 %), shoot number (3.43 AE 0.07) and shoot length (4.7 AE 0.31 cm) was obtained on Murashige and Skoog medium supplemented with 0.5 mg l À1 BAP and 1.0 mg l À1 TDZ. The shoots exhibited high frequency rhizogenesis on half strength medium augmented with 2.0 mg l À1 IAA. In vitro plantlets developed highest rate of photosynthesis on day 18 after the initiation of rhizogenesis. High survival rate (96.16%) under ex vitro conditions was observed when in vitro plantlets having high photosynthetic efficiency (Fv/Fm > 0.75) were subjected to hardening and acclimatization process. Plantlets with reduced photosynthetic performance exhibited low survival rate under natural conditions. The developed in vitro protocol will be useful for genetic improvement and multiplication of D. unguis-cati. The results of this study also show that photosynthetic screening of in vitro developed plantlets is highly essential after the rhizogenesis process to achieve higher survival rate under field conditions.

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Section: Resultssupporting

confidence: 47%

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

Soni

Keswani

Bhatt

et al. 2021

Heliyon

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“…These results highlight the superior effect of TDZ for the shoot regeneration of mesophyll protoplast-derived calli, which was also reported for apple [ 16 ], mulberry [ 17 ], Tylophora indica [ 55 ], and field cress [ 56 ]. In plant tissue cultures, the synthetic cytokinin-like phytohormone TDZ has been demonstrated to be the most effective agent for inducing shoot formation [ 57 , 58 ]. It has been reported that the cytokinin/auxin ratio is an essential factor in shoot regeneration [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

First Report on Mesophyll Protoplast Isolation and Regeneration System for the Duboisia Species

Xue,

Hiti-Bandaralage,

et al. 2023

Plants

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The Duboisia species, a group of plants native to Australia, have been historically valued for their pharmacological properties and have played a significant role in traditional medicine and pharmaceutical research. Persistent efforts are underway to enhance the efficacy of the active ingredient scopolamine, employing both conventional breeding methods and advanced biotechnology tools. The primary objective of this research was to establish a highly efficient method for isolating mesophyll protoplasts and facilitating their regeneration, thereby laying a robust foundation for the application of various advanced plant biotechnology tools in the pursuit of genetic enhancement. The mesophyll protoplast isolation process was developed for hybrid D. myoporoides × D. hopwoodii with careful optimisation of the following parameters: leaf strip size; incubation conditions; physical treatment; and enzyme concentration. The optimal parameters were combined in each individual step; the best enzyme concentration was determined to be 2% (w/v) cellulysin and 0.5% (w/v) macerase. Protoplast yield was found to be greatly affected by the enzyme concentrations. The isolated protoplasts were cultured at a density of 0.5 × 105 to best sustain the highest cell division (33.2%) and a microcalli induction frequency of 17.9%. After 40 days of culture in a modified KM8P medium at 25 °C in darkness, visible microcalli were transferred to a solidified Murashige and Skoog (MS) medium with 1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) for callus induction under a 16 h photoperiod. After 30 days of culture, compact organogenic calli were transferred into a solid MS medium with 6-benzylaminopurine (BA) alone or thidiazuron (TDZ) alone or in combination with BA or naphthalene acetic acid (NAA) for shoot regeneration. The maximum shoot regeneration frequency (63.3%) was observed in the medium with 1.5 mg L−1 TDZ alone. For the first time, a reliable protoplast isolation and regeneration system from mesophyll cells was established for Duboisia with high protoplast viability, successful microcalli formation, and intact plant regeneration. This innovation will significantly contribute towards the genetic enhancement of the Duboisia species.

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“…TDZ is a substituted phenyl urea, originally being used as a cotton defoliant. Compared to other PGRs TDZ exhibits both high auxin and cytokinin activity [52]. When used in plant tissue culture, it initiates adventitious bud and shoot formation and may also inhibit shoot elongation [53].…”

Section: Effect Of Pgrs On Micro-plantlets During Preculturementioning

confidence: 99%

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

Королева

Molkanova

Vysotskaya

2023

IJPB

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Cryopreservation is considered to be one of the most effective methods for long-term storage of plant genetic resources, particularly for ornamental species. However, there is a very little research on cryopreservation of lilacs. In this study, for the first time the cryopreservation protocol (a variation of a pregrowth-dehydration method) was successfully applied to two cultivars of Syringa vulgaris: ‘Aucubaefolia’ and ‘Polina Osipenko’. Explants of both cultivars were able to withstand the different steps of the protocol, and high survival and regrowth percentages were obtained after exposure to liquid nitrogen (67–100% and 63–88%, respectively). The current study is mainly focused on the preculture conditions of the applied method. Based on our results, we propose the use of paclobutrazol (PBZ) with the combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the preculture medium for increasing explant tolerance to subsequent dehydration and freezing. During post-LN recovery, the explants appeared morphologically normal, and after 12–16 weeks after thawing, they were propagated and cultured as normal plantlets. Therefore, the reported method is effective for long-term storage of lilac meristems and could be used to create a cryobank of achievements in lilac breeding.

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TDZ-Induced Morphogenesis Pathways in Woody Plant Culture

Cited by 16 publications

References 125 publications

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

First Report on Mesophyll Protoplast Isolation and Regeneration System for the Duboisia Species

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

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