TDZ Induced Diverse In Vitro Responses in Some Economically Important Plants

Giridhar, P.; Sridevi, V.; Matam, Pradeep; Shreelakshmi, Saligrama Viswanath

doi:10.1007/978-981-10-8004-3_17

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…One of the most effective cytokinin-like agents for encouraging cell division in tissue culture is TDZ [31,32], in addition to being more effective than BA, resulting in a greater number of multiple shoots per plant [33]. Generally, TDZ is used at lower concentrations in plant tissue culture [34], the low concentrations have reported to be effective in shoot organogenesis [35]. However, in this study, the 5 mg/L has proven to be the best concentration to produce the highest number of shoots.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Shalan¹,

Soliman²,

Mahmoud³

et al. 2023

Phyton

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Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication. In this research, nine multiplication media (MM) augmented with different concentrations of 6-benzyl adenine (BA), Kinetin (Kin), and Thidiazuron (TDZ), Three rooting media (RM) supplemented with three levels of α-naphthalene acetic acid (NAA) and three types of soil mixtures (v/v); Coco peat/Vermiculite/Sand (CVS), Peat moss/Perlite/Sand (PPS) and Peat moss/Perlite (PP) were used in the micropropagation protocol of daylily plants. MM2 showed the maximum shoot length and the number of leaves, while MM9 showed the maximum number of shoots. The RM1 showed the maximum root length and the number of roots. During acclimatization, CVS, PPS, and PP soil mixture showed similar performance except the CVS mixture showed lower performance regarding plant height and diameter. The genetic fidelity of micropropagated plants was evaluated using Start Codon Targeted (SCoT) Markers. Six SCoT primers amplified 51 scorable bands with an approximate range from 146 bp to 1598 bp size. Thirty one out of 51 loci were presented in the mother plants. 40 loci were polymorphic, 11 were monomorphic and 7 were unique. The amplification patterns of the micropropagated plants demonstrated genetic integrity to the mother plant ranging from 84.32 to 47.06 and somaclonal variations ranging from 52.94 with 5 mg/l BA pathway to 15.68 with 1mg/l TDZ pathway, thus demonstrating that the homogeneity and the variation of the micropropagated plants affected by the type and the quantity of the plant growth regulator used during multiplication subcultures. This research can be successfully used for other ornamental and medicinal plants' bulk multiplication, germplasm conservation, and future genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Shalan¹,

Soliman²,

Mahmoud³

et al. 2023

Phyton

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show abstract

“…Generally, TDZ is used at lower concentrations to effect plant micropropagation [23,26,27,38]. The concentrations smaller than 1 nM have proven to be effective in inducing shoot organogenesis [39]. However, it is evident in these studies that even at a concentration several folds greater than frequently applied [40][41][42], TDZ produced even greater results.…”

Section: Discussionmentioning

confidence: 99%

High frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron

Matand¹,

Shoemake

2020

BMC Plant Biol

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Background: Daylilies are a lucrative crop used for its floral beauty, medicinal proprieties, landscaping, fire prevention, nutritional value, and research. Despite the importance, daylilies remain extremely challenging for multiplying in vitro. The response difficulty is exacerbated because a few good protocols for daylilies micropropagation are generally difficult to reproduce across genotypes. An efficient strategy, currently applied at Langston University, is to systematically explore individual tissues or organs for their potential to micropropagation. This article is a partial report of the investigation carried out under room environmental conditions and focuses on developing an efficient daylilies in vitro propagation protocol that uses the stem tissue as the principal explant. Results: In less than three months, using thidiazuron, the use of the stem tissue as the in vitro experimental explant was successful in inducing multiple shoots several folds greater than current daylilies shoot organogenesis protocols. The study showed that tissue culture can be conducted successfully under unrestricted room environmental conditions as well as under the controlled environment of a growth chamber. It also showed that splitting lengthwise stem explants formed multiple shoots several folds greater than cross-sectioned and inverted explants. Shoot conversion rate was mostly independent of the number of shoots formed per explants. The overall response was explant and genotype-dependent. Efficient responses were observed in all thidiazuron treatments. Conclusion: An efficient protocol, which can be applied for mass multiple shoots formation using the daylilies stem tissue as the main explant, was successfully developed. This could lead to a broad and rapid propagation of the crop under an array of environmental conditions to meet the market demand and hasten exogenous gene transfer and breeding selection processes.

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Novel Plant Growth Regulators in In Vitro Establishment of Horticulture and Plantation Crops

Chugh

Kumar

2022

Commercial Scale Tissue Culture for Horticulture and Plantation Crops

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TDZ Induced Diverse In Vitro Responses in Some Economically Important Plants

Cited by 4 publications

References 29 publications

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

High frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron

Novel Plant Growth Regulators in In Vitro Establishment of Horticulture and Plantation Crops

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