tDNAser sequences are involved in the excision of Streptomyces griseus plasmid pSG1

Bar-Nir, Dror; Cohen, Amnon; Goedeke, Maria E.

doi:10.1016/0378-1119(92)90033-l

Cited by 12 publications

(9 citation statements)

References 32 publications

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“…Thus the bacteriophage ~CTX of tRNA genes and pathogenicity islands 115 Pseudomonas aeruginosa, which codes for a cytotoxin, uses a serine-specific tRNA locus as an attachment site (Hayashi et aL, 1993). Certain plasmids of Streptomyces coelicolor and Streptomyces griseus are able to integrate into the chromosome via tyrosine-and serine-specific tRNA genes (Reiter et aL, 1989;Mazodier et aL, 1990;Bar-Nir et aL, 1992;VSgtli and Cohen, 1992). In eukaryotic organisms, tRNA loci represent target structures for mobile genetic elements (Marschalek et aL, 1989;Chen and Fonzi, 1992).…”

Section: Discussionmentioning

confidence: 99%

tRNA genes and pathogenicity islands: influence on virulence and metabolic properties of uropathogenic Escherichia coli

Ritter¹,

Blum²,

Emödy

et al. 1995

Molecular Microbiology

109

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The uropathogenic Escherichia coli strain 536 (O6:K15:H31) carries two unstable DNA regions on its chromosome which were termed pathogenicity islands (Pais). Both pathogenicity islands, Pai I and Pai II, are incorporated into tRNA specific loci: Pai I is located in the tRNA gene for selenocysteine (selC), and Pai II is integrated in the leucine-specific tRNA locus leuX. Mutant strain 536-21 has lost the two pathogenicity islands together with the intact tRNA genes. While 536 is a virulent strain, 536-21 has lost a number of properties, including in vivo virulence. In previous publications we reported that the genes coding for two haemolysins (hly I, hly II) and P-related fimbria (prf) are located on the Pais. In this paper, we demonstrate that the expression of other gene products influencing metabolic properties in addition to in vivo virulence are strongly dependent on the intact tRNA loci selC and leuX. In order to determine the influence of the two tRNAs on the expression of these properties, the genes selC and leuX were cloned from the genome of strain 536 and then introduced into the mutant 536-21. Our results clearly show that the seleno-cysteine-specific tRNA (tRNA(Sec)) directly influences the ability of the bacteria to grow under anaerobic conditions, because selenocysteine is part of the enzyme formate dehydrogenase (FDH) which is involved in mixed acid fermentation. The rare leucine-specific tRNA5(Leu), encoded by leuX, influences a number if properties including type 1 fimbria production, flagellation and motility, production of enterobactin and serum resistance, and is also necessary for full in vivo virulence. While the tRNA(Sec) is directly involved in the production of FDHs, the leuX specific tRNA5(Leu) appears to influence the expression of various factors through specific transcriptional or translational control mechanisms.

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Section: Discussionmentioning

confidence: 99%

tRNA genes and pathogenicity islands: influence on virulence and metabolic properties of uropathogenic Escherichia coli

Ritter¹,

Blum²,

Emödy

et al. 1995

Molecular Microbiology

109

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“…Some of these plasmids have been shown to exist either as chromosomal or extra-chromosomal elements [42,43]. Strain YSH6000 itself harbours a 230-kb virulence plasmid that is able to insert spontaneously into the metB gene on the chromosome resulting in loss of virulence.…”

Section: Mapping Of the Deletion On The S Flexneri Chromosomementioning

confidence: 99%

A spontaneous 99-kb chromosomal deletion results in multi-antibiotic susceptibility and an attenuation of contact haemolysis in Shigella flexneri 2a

Rajakumar

Sasakawa

Adler

1996

Journal of Medical Microbiology

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A Tn5-generated mutant (strain S2430) of Shigella flexneri 2a (strain YSH6000) exhibited attenuated virulence and, in addition to the Tn5 insertion in the SalI K fragment of its virulence plasmid, had a 99-kb deletion within its chromosome. Unlike its wild-type parent, strain S2430 was susceptible to ampicillin, streptomycin, tetracycline and chloramphenicol. An independent multi-antibiotic susceptible variant of strain YSH6000 had a similar deletion. Southern blot analysis of pulsed field electrophoresis gels enabled the sizing of this deletion and its mapping to a region of the chromosome on Not1 fragment D bounded by the S. flexneri homologues of ompA and pyrC. Hybridisation experiments with a probe specific to the multi-antibiotic resistance region indicated that this large deletion was responsible for antibiotic susceptibility. Both strain S2430 and a derivative of the antibiotic-susceptible variant, with a Tn5 insertion in its SalI K fragment, exhibited an equal reduction in contact haemolysis compared with the Tn5-bearing derivative of strain YSH6000. However, strain S2430 alone clearly displayed delayed plaque forming ability in LLC-MK2 monolayers, suggesting that the two examples of this deletion may not be identical.

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“…Current evidence indicates that attB sites are contained within tRNA genes (3,6,9,22,26). A database screen revealed that nucleotides 1780 through 1824, located downstream of int (Fig.…”

Section: Gtacgtacccacgaatcccgtagcggctgtcaaggctccgtccccggagaatcgaggcqamentioning

confidence: 99%

A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration

1994

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tDNAser sequences are involved in the excision of Streptomyces griseus plasmid pSG1

Cited by 12 publications

References 32 publications

tRNA genes and pathogenicity islands: influence on virulence and metabolic properties of uropathogenic Escherichia coli

tRNA genes and pathogenicity islands: influence on virulence and metabolic properties of uropathogenic Escherichia coli

A spontaneous 99-kb chromosomal deletion results in multi-antibiotic susceptibility and an attenuation of contact haemolysis in Shigella flexneri 2a

A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration

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