TDI inhalation in guinea-pigs involves migration of dendritic cells

Ban, M.; Hettich, D.; Goutet, Michèle; Bonnet, Pierre

doi:10.1016/s0378-4274(97)00091-x

Cited by 17 publications

(14 citation statements)

References 23 publications

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“…The findings of these studies also indicated that a short period of repeated exposure to TDI caused respiratory hypersensitivity in which airway inflammatory and immunological events, represented in part by eosinophil infiltration and increases in the levels of select T H 2 cytokines, may have played an important role. These latter findings build upon those from a study by Ban et al (1997) in which it was noted that the levels of TNFα and IL-6 were increased in the BAL fluid of pigs after exposure to TDI.…”

Section: Discussionsupporting

confidence: 81%

Effect ofMoringa oleiferaLam. Seed Extract on Toluene Diisocyanate-Induced Immune-Mediated Inflammatory Responses in Rats

Mahajan

Mali

Mehta

2007

Journal of Immunotoxicology

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Moringa oleifera Lam. is a small tree cultivated throughout India. We have investigated the effect of ethanolic extract of seeds of Moringa oleifera (MOEE, an herbal remedy) on the potential prevention of immune-mediated inflammatory responses in toluene diisocyanate (TDI as antigen)-induced asthma in Wistar rats. Rats were divided into five different groups (n = 8/group): Group-I = unsensitized control; Group-II = TDI control/vehicle; Group-III = dexamethasone (DXM) 2.5 mg/kg; and, Groups IV and V = 100 mg/kg and 200 mg/kg body weight [BW] of MOEE, respectively. All rats (except unsensitized controls) were sensitized by intranasal application of 10% TDI to induce airway hypersensitivity. Animals in Groups II-V were given their respective drug treatment per os from 1 wk prior to initiation of sensitization until the day of final provocation with 5% TDI. After this last challenge, all rats were examined for hyperreactivity symptoms and then sacrificed to determine their total and differential leucocytes in blood and bronchoalveolar (BAL) fluid and levels of TNF∝, IL-4, and IL-6 in their BAL and serum. Homogenates of one lung lobe from each animal were utilized to assess oxidative stress; a separate lobe underwent histologic examination to assess airway inflammatory status. The results suggest that asthmatic symptoms were found in TDI control rats only, while both MOEE-and DXM-treated rats did not manifest any airway abnormality. In MOEE-and DXM-treated rats, neutrophil and eosinophil levels in the blood were decreased significantly; levels of total cells and each different cell in their BAL fluid were markedly decreased as compared to those in TDI controls. TNFα, IL-4, and IL-6 were predominant in serum as well as in BAL fluids in TDI controls, but these levels were reduced significantly by MOEE treatment. The antioxidant activity in relation to antiinflammatory activity of the extract and histopathological observations also reflected a protective effect. Based on the above findings and observations, it can be concluded that Moringa oleifera may possess some beneficial properties that act against chemically stimulated immune-mediated inflammatory responses that are characteristic of asthma in the rat.

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Section: Discussionsupporting

confidence: 81%

Effect ofMoringa oleiferaLam. Seed Extract on Toluene Diisocyanate-Induced Immune-Mediated Inflammatory Responses in Rats

Mahajan

Mali

Mehta

2007

Journal of Immunotoxicology

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“…Recent studies showed that after Toluene Diisocyanate (TDI) inhalation in a guinea pig model, the number of dendritic cells increased in lungs approximately 24 hours after exposure to the hapten. A few hours later, the number of DCs decreased in the airways (Ban et al, 1997). In DC hu-SCID mice, dendritic cells were also located in lymphoid organs of the mice: the spleen and the thymus of the animals.…”

Section: Hammad Et Almentioning

confidence: 97%

Human Dendritic Cells in the Severe Combined Immunodeficiency Mouse Model: Their Potentiating Role in the Allergic Reaction

Hammad

Duez

Fahy

et al. 2000

Laboratory Investigation

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SUMMARY:Dendritic cells (DCs) are present in the lungs and airways of healthy and allergic subjects where they are exposed to inhaled antigens. After the uptake of antigens, DCs migrate to lymphoid organs where T cells initiate and control the immune response. The migratory properties of DCs are an essential component of their function but remain unclear in the situation of allergic diseases. To better understand the role of DCs in response to allergens, we first investigated their presence in an original experimental model of allergic asthma: the humanized severe combined immunodeficiency (SCID) mouse reconstituted with peripheral blood mononuclear cells from patients sensitive to Dermatophagoides pteronyssinus (Dpt). Human DCs were detected in lungs of mice developing an inflammatory pulmonary infiltrate and appeared to be mainly located in the alveolar spaces. In a second step, human DCs were generated in vitro from monocytes and injected into naive SCID mice exposed or not exposed to Dpt aerosols. Their migratory behavior was explored, as well as their potential role in modulating the IgE production after exposure to Dpt. After exposure to Dpt, the number of DCs present in airways decreased, while it increased into the spleen and thymus of the mice. The IgE production increased in the presence of DCs as compared with mice not injected with DCs. These results suggest that DCs may play a role in the pulmonary allergic reaction developed in response to Dpt in SCID mice. (Lab Invest 2000, 80:605-614).

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“…The techniques of atmosphere generation and atmosphere sampling and analysis of TDI were described in detail in our previous study (Ban et al, 1997). In the control group, the mice were exposed to sterile air in inhalation chambers.…”

Section: Inhalation/inhalation Design (1)mentioning

confidence: 99%

“…The latter may be recognised, and taken up by professional antigen presenting cells (e.g. dendritic cells in the airway epithelium) before being transported and presented later to the naïve T cells in the lungassociated lymph nodes (Ban et al, 1997). TDI is capable of inducing different types of immune reactions, depending on the polarization of the T cells toward the helper T type 1 (Th1) or helper type 2 (Th2) cells.…”

Section: Introductionmentioning

confidence: 99%