TdCBL6, a calcineurin B-like gene from wild emmer wheat (Triticum dicoccoides), is involved in response to salt and low-K+ stresses

Chen, Liang; Ren, Jing; Shi, Haiyan; Zhang, Yongkuan; You, Yu; Fan, Jibiao; Chen, Ke; Liu, Shuqian; Nevo, Eviatar; Fu, Jinming; Peng, Junhua

doi:10.1007/s11032-015-0229-1

Cited by 11 publications

(9 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of AtKEA5 , expression is induced by NaCl and ABA treatments as compared to that in untreated controls (Zheng et al, 2013). However, the localization of KEA5 in plants remains unknown (Chen et al, 2015). SsAKT1 , encoding an inward potassium channel, was expressed at high levels in the flowers of S. salsa plants treated with NaCl in our study (Figure 7), as did the homolog in Arabidopsis, whose protein product has been confirmed to conduct K + uptake under low K + conditions (Wang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Salt-Enhanced Reproductive Development of Suaeda salsa L. Coincided With Ion Transporter Gene Upregulation in Flowers and Increased Pollen K+ Content

et al. 2019

View full text Add to dashboard Cite

Halophytes are adapted to saline environments and demonstrate optimal reproductive growth under high salinity. To gain insight into the salt tolerance mechanism and effects of salinity in the halophyte Suaeda salsa , the number of flowers and seeds, seed size, anther development, ion content, and flower transcript profiles, as well as the relative expression levels of genes involved in ion transport, were analyzed in S. salsa plants treated with 0 or 200 mM NaCl. The seed size, flower number, seed number per leaf axil, and anther fertility were all significantly increased by 200 mM NaCl treatment. The Na + and Cl − contents in the leaves, stems, and pollen of NaCl-treated plants were all markedly higher, and the K + content in the leaves and stems was significantly lower, than those in untreated control plants. By contrast, the K + content in pollen grains did not decrease, but rather increased, upon NaCl treatment. Genes related to Na + , K + and, Cl − transport, such as SOS1 , KEA , AKT1 , NHX1 , and CHX , showed increased expression in the flowers of NaCl-treated plants. These results suggest that ionic homeostasis in reproductive organs, especially in pollen grains under salt-treated conditions, involves increased expression of ion transport-related genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Salt-Enhanced Reproductive Development of Suaeda salsa L. Coincided With Ion Transporter Gene Upregulation in Flowers and Increased Pollen K+ Content

et al. 2019

View full text Add to dashboard Cite

show abstract

“…CBL2 and CBL3 proved to affect seed size and embryonic development by transgenic operation in Arabidopsis [18]. However, up to date, functional characterization of CBL6 merely reported in Stipa purpurea and Triticum dicoccoides [30], all of which provided novel insight but just catch a little glimpse of its talents. In this study, we enforced functional analysis for ScCBL6 by ectopic expression in Arabidopsis, followed by comparative transcriptome analysis between its wild type (WT) and transgenic lines as regard to cold stress response.…”

Section: Discussionmentioning

confidence: 99%

Cold tolerance of ScCBL6 is associated with photosynthesis and tonoplast transporters in Arabidopsis

Zhou¹,

Zhao²,

Long³

et al. 2019

Preprint

View full text Add to dashboard Cite

Plant adapted in the fragile zone offer enormous opportunity to understand the stress biology under ecological scenario . Stipa capillacea is widely distributed in the frigid and arid region of Tibet Plateau, but its signal system has never been investigated yet. In this study, we isolated a signal transduction gene, ScCBL6 , in Stipa capillacea , to characterize its cold tolerance capacity by ectopic expression in Arabidopsis . The results suggested that full length ScCBL6 encodes 227 amino acids, and phylogenetically clustered with CBL6 protein in Stipa purpurea and Oryza sativa . In comparison with wild type (WT) plants, ScCBL6 overexpressing plants ( ScCBL6- OXP) are tolerant to cold stress but not drought stress, which attested by the higher photosynthetic capacity (Fv/Fm) and survival rate of ScCBL6- OXP under cold stress. We further compared their cold-responsive transcriptome profiles through RNA-Seq. Totally, 3931 genes were differentially expressed by introduction of ScCBL6. They are participated in multiple processes like immune system, lipid catabolic, secondary metabolic and mainly enriched in plant hormone signal transduction and biomacro-molecule metabolism as regard to KEGG pathway. Differential expressed genes (DEGs) were predicted to locate in chloroplast, mitochondrion, vacuole, and so on, suggesting multitudinous function of ScCBL6. Based on the integrated analysis of ScCBL6-OXP, we inferred that ScCBL6 improve plant cold stress tolerance via regulate photosynthesis redox and vacuole metabolites transport in Arabidopsis .

show abstract

“…Notably, a thorough search within and between TD populations can identify superior salt‐resistant genotypes, such as Gitit 18–35, which is better in relative dry weight, electrolyte leakage, and salt‐resistant genes (NAC2F, NAC 8, DREB3A, and MYB 2A) than wheat cultivar Zheng 9023. A clear demonstration on how to seek elite genotypes links the discoveries of salt‐resistant genotypes in TD by Nevo et al (), continued by Shavrukov et al () and complemented by Chen et al (, ). Future exploration of wild emmer could unravel many additional salt‐ and drought‐resistant elite genes for breeding.…”

Section: Introductionmentioning

confidence: 93%

“…and Fig. S12, involve physiological and molecular responses to salt stress (Chen et al, , ). Salt stress was examined in the superior salt‐tolerant Gitit genotype 18–35 from Gitit, a site in the hot and dry eastern Samaria steppic mountains facing the Jordan Valley.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evolution of wild emmer wheat and crop improvement

Nevo

2014

J of Sytematics Evolution

Self Cite

View full text Add to dashboard Cite

Allotetraploid wild emmer wheat, Triticum dicoccoides (TD) 2n ¼ 28, the progenitor of most cultivated bread wheat, is an ecological specialist and excellent model organism for advancing evolutionary theory, wheat evolution, and wheat improvement. The center of origin and diversity of TD is northeastern Upper Galilee and the Golan. Elsewhere in the Fertile Crescent, it occurs in semi-isolated and isolated populations. The genetic structure is generally an "archipelago." Regional and local genetic patterns are partly or largely adaptive at the protein and DNA levels, both at coding and noncoding genomes, correlated with and predictable by environmental abiotic and biotic stresses. TD is a rich, mostly untapped, genetic resource for improving cultivated wheat, harboring drought, salt, mineral, and disease resistances, grain proteins, and with high variation in photosynthetic yield. TD was chromosomally mapped with 549 molecular markers and 70 QTLs for 11 traits of agricultural importance and domestication. Prospects include sequencing its genome; including 80% repeat elements for structural, functional, and regulatory polymorphisms, epigenetics, and genetic resources for wheat improvement. TD is affected by global warming both phenotypically (advancing flowering time) and genotypically (genetic erosion, SSR allelic turn-over, and novel drought resistant alleles). Hence, it should be conserved in situ and ex situ, to safeguard the arguably best source for wheat improvement and future food production in an exploding world population.

show abstract

TdCBL6, a calcineurin B-like gene from wild emmer wheat (Triticum dicoccoides), is involved in response to salt and low-K+ stresses

Cited by 11 publications

References 54 publications

Salt-Enhanced Reproductive Development of Suaeda salsa L. Coincided With Ion Transporter Gene Upregulation in Flowers and Increased Pollen K+ Content

Salt-Enhanced Reproductive Development of Suaeda salsa L. Coincided With Ion Transporter Gene Upregulation in Flowers and Increased Pollen K+ Content

Cold tolerance of ScCBL6 is associated with photosynthesis and tonoplast transporters in Arabidopsis

Evolution of wild emmer wheat and crop improvement

Contact Info

Product

Resources

About