TCR Spectratyping in Transplantation

Haarer, Jan

doi:10.1097/tp.0000000000001015

Cited by 1 publication

(2 citation statements)

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“…The analysis of TCR diversity through spectratyping in our patients suggested that renal transplant recipients who experienced graft failure were characterized by more persistent expanded T-cell clones, with a potential specific role in the acceptance of transplanted solid organs 27,29. Previous evidence has indicated that the extent of skewing of TCR repertoires and levels of clonal T-cell expansion are significantly correlated with renal transplant outcomes.…”

Section: Discussionmentioning

confidence: 66%

“…Spectratyping is a method able to evaluate T-cell-repertoire complexity through CDR3-size heterogeneity of the TCR β-chain, and represents a more powerful approach than lymphocyte-subset analysis through flow cytometry, for the clinical immunomonitoring of solid-organ recipients 25–27. The CDR3 sequence defines a unique clonotype that behaves as a fingerprint for the T-cell line bearing it.…”

Section: Introductionmentioning

confidence: 99%

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Identification of expanded T-cell clones by spectratyping in nonfunctioning kidney transplants

Cappuccilli

Donati

Comai

et al. 2017

JIR

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BackgroundThe aim of this study was the application of complementarity-determining region-3 spectratyping analysis to determine T-cell-repertoire complexity and to detect T-cell-clone expansion, as a measure of immune response in nonfunctioning kidney transplants (group hemodialysis-transplant [HD-Tx]), nontransplanted dialysis patients (group hemodialysis [HD]), and normal subjects as controls (group C).Patients and methodsAnalysis of T-cell receptor (TCR) diversity by spectratyping was applied to peripheral blood samples collected from 21 subjects: eight in group HD-Tx, seven in group HD, and six in group C.ResultsConsidering the extent of the skew in TCR variable region repertoires as a measure of clonal T cells, we found that the number of altered spectra showed a progressive increase from normal subjects to dialysis patients and to nonfunctioning kidney transplants, respectively. Healthy subjects had the lowest number of altered spectra, and patients with nonfunctioning kidney transplants the highest. Differences were significant for group HD-Tx vs group C (P=0.017) and group HD vs group C (P=0.015), but not between nonfunctioning kidney-transplant recipients and dialysis patients (group HD-Tx vs group HD).ConclusionAlthough dialysis appears to be a weaker trigger for clonal expansion of T cells, our data suggest that the utilization of complementarity-determining region-3 spectratyping analysis of the TCR repertoire might be useful to monitor specific immunoactivation in patients before and after kidney transplantation.

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%