TCP Transcription Factors in Moso Bamboo (Phyllostachys edulis): Genome-Wide Identification and Expression Analysis

Abstract

TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (T), members of a plant-specific gene family, play significant roles during plant growth and development, as well as in response to environmental stress. However, knowledge about this family in moso bamboo (Phyllostachys edulis) is limited. Therefore, in this study, the first genome-wide identification, classification, characterization, and expression pattern analysis of the TCP transcription factor family in moso bamboo was performed. Sixteen TCP members were identified from the moso bamboo genome using a BLASTP algorithm-based method and verified using the Pfam database. Based on a multiple-sequence alignment, the members were divided into two subfamilies, and members of the same family shared highly conserved motif structures. Subcellular localization and transactivation activity analyses of four selected genes revealed that they were nuclear localized and had self-activation activities. Additionally, the expression levels of several PeTCP members were significantly upregulated under abscisic acid, methyl jasmonate, and salicylic acid treatments, indicating that they play crucial plant hormone transduction roles in the processes of plant growth and development, as well as in responses to environmental stresses. Thus, the current study provides previously lacking information on the TCP family in moso bamboo and reveals the potential functions of this gene family in growth and development.

Genome-Wide Characterization and Gene Expression Analyses of GATA Transcription Factors in Moso Bamboo (Phyllostachys edulis)

“…Moreover, bamboo is known for its fast-growing shoots and widespread rhizomes [2]. It has been reported that several gene families are involved in flower development and abiotic stress [30][31][32]37]; however, the rapid-growth associated transcription factors remain elusive. The genome sequences of moso bamboo [36] and transcriptome studies [2,27,28,34] provide important platforms for the identification of rapid-growth shoot and rhizome development associated gene families.…”

“…Recently, large-scale transcriptome analysis has shown that light and phytohormones may play important roles in the rapid-growth of bamboo [27][28][29]. In addition, a large number of transcription factor families, involved in the abiotic stress response and flower development, have been studied in moso bamboo [30][31][32]. Although a previous study has functionally characterized the rapid-growth associated gene-PeGSK1 [1], the rapid-growth associated transcription factor families are largely unexplored in moso bamboo.…”

Genome-wide analysis of GATA factors in moso bamboo (Phyllostachys edulis) unveils that PeGATAs regulate shoot rapid-growth and rhizome development

“…Moreover, bamboo is known for its fast-growing shoots and widespread rhizomes [2]. It has been reported that several gene families are involved in flower development and abiotic stress [30–32, 41], the rapid-growth associated transcription factors remain elusive. The genome sequences of moso bamboo[33] and transcriptome studies [2, 27, 28, 40] provide important platforms for the identification of rapid-growth shoot and rhizome development associated gene families.…”

“…Recently, large-scale transcriptome analysis has shown that light and phytohormones may play important roles in the rapid-growth of bamboo [27–29]. In addition, a large number of transcription factor families are involved in the abiotic stress response and flower development have been studied in moso bamboo [30–32]. Although our group has functionally characterized rapid-growth associated key gene- PeGSK1 , the rapid-growth associated transcription factor families are largely unexplored in moso bamboo.…”

Genome-wide identification and transcript analysis of TCP transcription factors in grapevine

“…To date, a number of TCP family members have been characterized in both dicots and monocots with the completion of entire genome, such as Arabidopsis [32], tomato [21], apple [33], strawberry [31], bamboo [34] and switchgrass [35]. However, little is known about the TCP family in grapevine [36], which is one of the most important fruit crop growing around the world with great nutritive and commercial value [37][38][39].…”