TcMYB1, TcMYB4, and TcMYB8 participate in the regulation of lignan biosynthesis in Taiwania cryptomerioides Hayata

Chiang, Nien-Ting; Wen, Chia-Hsien; Chu, Fang-Hua

doi:10.1007/s11295-019-1375-0

Cited by 4 publications

(6 citation statements)

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“…Eight MYB-and two NAC-encoding transcripts were included in our DEGs, and three MYB-and one NAC-encoding DEGs were up-regulated more than 13-fold in TZ compared to SW-1 (Figure 9; Table S6). MYB transcription factors are often involved in secondary metabolism pathway regulation [37,38], and both transcription factor families are closely related to secondary cell wall biosynthesis and PCD [39,40]. However, the BlastX-searching results of these highly expressed MYB-or NAC-encoding DEGs in TZ did not reveal further specific functions related to wood formation associated TFs (Tables S5 and S6).…”

Section: Transcription Factors and Programmed Cell Death During Hw Fomentioning

confidence: 99%

Differential Gene Profiling of the Heartwood Formation Process in Taiwania cryptomerioides Hayata Xylem Tissues

Yeh

Chu

Liu

et al. 2020

IJMS

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Taiwania (Taiwania cryptomerioides) is an important tree species in Taiwan because of the excellent properties of its wood and fascinating color qualities of its heartwood (HW), as well as the bioactive compounds therein. However, limited information is available as to the HW formation of this species. The objective of this research is to analyze the differentially expressed genes (DEGs) during the HW formation process from specific Taiwania xylem tissues, and to obtain genes that might be closely associated with this process. The results indicated that our analyses have captured DEGs representative to the HW formation process of Taiwania. DEGs related to the terpenoid biosynthesis pathway were all up-regulated in the transition zone (TZ) to support the biosynthesis and accumulation of terpenoids. Many DEGs related to lignin biosynthesis, and two DEGs related to pinoresinol reductase (PrR)/pinoresinol lariciresinol reductase (PLR), were up-regulated in TZ. These DEGs together are likely involved in providing the precursors for the subsequent lignan biosynthesis. Several transcription factor-, nuclease-, and protease-encoding DEGs were also highly expressed in TZ, and these DEGs might be involved in the regulation of secondary metabolite biosynthesis and the autolysis of the cellular components of ray parenchyma cells in TZ. These results provide further insights into the process of HW formation in Taiwania.

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Section: Transcription Factors and Programmed Cell Death During Hw Fomentioning

confidence: 99%

Differential Gene Profiling of the Heartwood Formation Process in Taiwania cryptomerioides Hayata Xylem Tissues

Yeh

Chu

Liu

et al. 2020

IJMS

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“…Based on the results obtained from our analysis we have classified MYB TFs into four categories each containing 2, 22, 92 and 163 members respectively (Figure 16). Recent study based on MYB TFs of Taiwania cryptomerioides has revealed that TcMYB1, TcMYB4, and TcMYB8 positively regulate lignan biosynthesis by activating the gene expressions of TcPLR3 and TcPLR1 respectively (Chiang et al, 2019). Results obtained from our phylogenetic analysis have showed 4 K. heteroclita MYBs KhMYB118, KhMYB37, KhMYB203 and KhMYB36 share the same clade S6 with TcMYB1, while KhMYB249, KhMYB167, KhMYB199, KhMYB4 and KhMYB145 are in the same S7 clade with TcMYB4 and TcMYB8 (Figure 16).…”

Section: Identification Of Transcription Factorsmentioning

confidence: 69%

“…For example, the substrates for LuPLR1 and TpPLR1 proteins are (-)pinoresinol and (-)-lariciresinol which leads to the product (+)-secoisolariciresinol (Fujita et al, 1999;von Heimendahl et al, 2005). Similarly, the substrates for LuPLR2, TcPLR2.2, TcPLR3, TpPLR2 and PpPLR proteins are (+)-pinoresinol, (+)-lariciresinol but the product is (-)-secoisolariciresinol respectively (Fujita et al, 1999;Hemmati et al, 2010;Chiang et al, 2019). It is still unknown that whether these two steps are catalyzed by one PLR or two different PLRs.…”

Section: Figure 7 Kegg Classification Mapmentioning

confidence: 99%

“…In summary, the majority of the above-mentioned TFs are highly upregulated in root and stem compared with leaf samples (Table 6). Recent studies have shown that lignan and terpenoid biosynthesis are highly regulated by MYBs (Deng and Lu, 2017;Matías-Hernández et al, 2017;Chiang et al, 2019). In order to understand and reveal the TFs involved in K. heteroclita's lignan and terpenoid biosynthesis, we have performed phylogenetic analysis of all 279 MYB TFs against 37 MYB TF sequences belonging to other plant species.…”

Section: Identification Of Transcription Factorsmentioning

confidence: 99%

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Transcriptome analysis to identify genes involved in lignan, sesquiterpenoid and triterpenoid biosynthesis in medicinal plant Kadsura heteroclita

Zhang

Chio

et al. 2020

Not Bot Horti Agrobo

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Stems and roots of Kadsura plant species were the significant ingredients of traditional Chinese medicine. Kadsura heteroclita is one of the popular medicinal plants used in Tujia and Yao nationalities of China. Antioxidant compounds like lignan, sesquiterpenoid and triterpenoid are the major active components of K. hetroclita. Mass cultivation and bio-manufacturing strategies were being proposed to meet the increasing demand of Kadsura species plant parts. Therefore, it is important to reveal the molecular networks involved in biosynthesis of these highly efficient medicinal compounds. Here, transcriptomes of roots, stems and leaves in K. heteroclite seedling were sequenced by Hiseq2000 and unigenes involved in biosynthesis of lignan, sesquiterpenoid and triterpenoid biosynthesis were mined. As a result, 472 million clean reads were obtained which after aligning resulted in 160,248 transcripts and 98,005 genes. 191 and 279 unigenes were expected to be involved in biosynthesis of lignan, sesquiterpenoid and triterpenoid biosynthetic pathways respectively. Lignan, sesquiterpenoid and triterpenoid biosynthesis pathway genes were highly significant and differentially upregulated in roots and stems and downregulated in leaves. Also, genes encoding for MYB and bHLH transcription factors possibly involved in regulation of lignan, sesquiterpenoid and triterpenoid biosynthesis were discovered. These results provide the fundamental genomic resources for dissecting of biosynthetic pathways of the active components in K. hetroclita.

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“…In addition, MYB, bHLH and ERF transcription factors play important regulatory roles in the lignan biosynthetic pathway. For example, TcMYB1, TcMYB4, and TcMYB8 are involved in the positive regulation of lignan biosynthesis in Taiwania cryptomerioides Hayata [ 46 ]. Among them, TcMYB1, TcMYB4, and TcMYB8 are associated with four MYBs (AtMYB20, AtMYB42, AtMYB43, and AtMYB85), two MYBs (AtMYB46 and AtMYB83), and two MYBs (AtMYB61 and AtMYB50) of Arabidopsis Thaliana clustered together in the same branch [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Tissue-specific transcriptome and metabolome analyses reveal candidate genes for lignan biosynthesis in the medicinal plant Schisandra sphenanthera

Sun,

Wang,

Guan

et al. 2023

BMC Genomics

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Schisandra sphenanthera is an extremely important medicinal plant, and its main medicinal component is bioactive lignans. The S. sphenanthera fruit is preferred by the majority of consumers, and the root, stem, and leaf are not fully used. To better understand the lignan metabolic pathway, transcriptome and metabolome analyses were performed on the four major tissues of S. sphenanthera. A total of 167,972,229 transcripts and 91,215,760 unigenes with an average length of 752 bp were identified. Tissue-specific gene analysis revealed that the root had the highest abundance of unique unigenes (9703), and the leaves had the lowest (189). Transcription factor analysis showed that MYB-, bHLH- and ERF-transcription factors, which played important roles in the regulation of secondary metabolism, showed rich expression patterns and may be involved in the regulation of processes involved in lignan metabolism. In different tissues, lignans were preferentially enriched in fruit and roots by gene expression profiles related to lignan metabolism and relative lignan compound content. Furthermore, schisandrin B is an important compound in S. sphenanthera. According to weighted gene co-expression network analysis, PAL1, C4H-2, CAD1, CYB8, OMT27, OMT57, MYB18, bHLH3, and bHLH5 can be related to the accumulation of lignans in S. sphenanthera fruit, CCR5, SDH4, CYP8, CYP20, and ERF7 can be related to the accumulation of lignans in S. sphenanthera roots. In this study, transcriptome sequencing and targeted metabolic analysis of lignans will lay a foundation for the further study of their biosynthetic genes.

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TcMYB1, TcMYB4, and TcMYB8 participate in the regulation of lignan biosynthesis in Taiwania cryptomerioides Hayata

Cited by 4 publications

References 50 publications

Differential Gene Profiling of the Heartwood Formation Process in Taiwania cryptomerioides Hayata Xylem Tissues

Differential Gene Profiling of the Heartwood Formation Process in Taiwania cryptomerioides Hayata Xylem Tissues

Transcriptome analysis to identify genes involved in lignan, sesquiterpenoid and triterpenoid biosynthesis in medicinal plant Kadsura heteroclita

Tissue-specific transcriptome and metabolome analyses reveal candidate genes for lignan biosynthesis in the medicinal plant Schisandra sphenanthera

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