TCF21 inhibits proliferation and chemoresistance through the AKT pathway in human gastric cancer

Yang, Zhi; Jiang, Xiaodi; Liu, Deming; Dong, Qian; Zhao, Haiying; Jiang, Xiaofeng

doi:10.1016/j.gene.2018.10.011

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…However, there are fewer reports of SCGB1A1, A2M, ACSL5 and MND1 in lung cancer. In vitro overexpression of TCF21 may inhibit tumor growth and chemoresistance possibly through the AKT signaling pathway (73,74). Upregulation of ITGB8 may promote the expression of tumor metastasis genes and enhance the invasive ability of tumor cells in LUSC by regulating the phosphorylation levels of mitogen-activated protein kinase/extracellular signal-regulated kinase and AKT.…”

Section: Discussionmentioning

confidence: 99%

Identification of potential diagnostic and therapeutic target genes for lung squamous cell carcinoma

et al. 2019

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The purpose of this study was to identify potential molecular markers of lung squamous cell carcinoma (LUSC). Three datasets containing LUSC mRNA sequencing data were downloaded from the Gene Expression Omnibus, The Cancer Genome Atlas and the Gene Expression Profiling Interactive Analysis databases. These datasets were used to identify significantly differentially expressed genes (DEGs) in LUSC. A protein-protein interaction network of the DEGs was constructed followed by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and overall survival analyses of the DEGs. A total of 37 DEGs between LUSC and normal tissues were identified, including 26 downregulated genes and 11 upregulated genes. Biological Process enrichment analysis revealed that the DEGs were mainly enriched in ‘cell adhesion’, ‘cell-matrix adhesion’, ‘anatomical structure morphogenesis’, ‘ECM-receptor interaction’ and ‘focal adhesion’. Overall survival analysis demonstrated that transcription factor 21, α-2-macroglobulin, acyl-CoA synthetase long chain family member 5, integrin subunit β8, meiotic nuclear divisions 1 and secretoglobin family 1A member 1 were significantly associated with the occurrence and development of lung cancer, and these genes were selected as hub genes. The results obtained in the present study may aid the elucidation of the molecular mechanisms involved in the development of LUSC and may provide potential targets for LUSC treatment.

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Section: Discussionmentioning

confidence: 99%

Identification of potential diagnostic and therapeutic target genes for lung squamous cell carcinoma

et al. 2019

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“…MEF2C was also explored regulated by miRNAs, including miR-802, miR-194, and miR-190b [39,40]. Transcription factor 21 (TCF21), a member of the basic helix-loop-helix (bHLH) transcription factors, was methylated and downregulated and inhibited gastric cancer growth and chemoresistance possibly through the AKT signaling pathway in and served as an independent prognostic factor [41]. Promoter methylation of TCF21 was frequently observed and might repress autophagy in the early stage of non-small cell lung cancer (NSCLC) [42].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Epigenetic Alternations of MicroRNA and DNA Methylation Contribute to Gene Expression and Prognosis in Lung Squamous Cell Carcinoma

Liang

Wang

2022

Preprint

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Background Early detection of lung squamous cell carcinoma (LUSC) is significantly effective in clinical management. This study aimed to identify potential LUSC biomarkers based on the epigenetic alterations of miRNAs and DNA methylation. Methods Analysis of expression profiles in LUSC clinical samples downloaded from The Cancer Genome Atlas (TCGA-LUSC) datasets was performed to identify differentially expressed genes (DEGs) using R packages. The biological functions were annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The protein-protein interaction (PPI) network of the DEGs was established through STRING (Search Tool for the Retrieval of Interacting Genes database) website, visualized by Cytoscape and further analyzed by Molecular Complex Detection (MCODE). Kaplan-meier method and univariate Cox proportional risk regression analysis were used to screen differential genes related to survival in LUSC patients. Multivariate Cox proportional risk regression analysis was used to construct a prognosis prediction model of LUSC patients with differentially expressed genes. Receiver operating characteristic (ROC) curve analysis confirmed the biomarker's specific and sensitivity. Results We systematically identified 96 DEGs modified by both DNA methylation and miRNA through TCGA dataset between LUSC tissues and normal tissues. KEGG pathway and GO enrichment analysis gave us an insightful view of the functions of DEMs and DMGs. Subsequently, the four-gene prognosis model were obtained, including SMAD7, MEF2C, TCF21, and TRIB1, together with clinical factors, smoking history and EvsA. Survival curve analysis indicates that the model has good predictive value for the prognosis of patients with LUSC. We also found their differential expression, especially MEF2C and TCF21, together with immune infiltration levels in B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells, correlated with LUSC prognosis. Conclusions Four DEGs modified by both DNA methylation and miRNA were associated with the overall survival of LUSC patients and could be potential biomarkers to predict prognosis. MEF2C and TCF21 likely play important roles in immune cell infiltration and as prognosis biomarkers in LUSC patients with immune cell infiltration. In future research, we will explore the prognostic genes and their potential function based on the present study.

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“…Cell proliferation is a normal physiological process occurring in every tissue of the body (33). Under normal conditions, cell proliferation and programmed cell death exist in a balance (34). However, in gastric cancer cells, this balance is disrupted by uncontrolled cell proliferation (35).…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides from an Ophiopogon japonicus endophyte inhibit proliferation and migration in MC-4 human gastric cancer cells

Xu¹,

Yang²,

Yang³

et al. 2018

Transl. Cancer Res

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Background: Recently, natural products have gained increasing attention. Endophytic bacteria represent a source of natural anticancer products, and are well-known for producing diverse bioactive anti-cancer compounds. Herein, we investigated the antitumor activity of exopolysaccharides (EPSs) extracted from endophytes in Ophiopogon japonicus.Methods: The endophyte bacterium MD4 was isolated from O. japonicus. To identify MD4, a partial sequence of 16S rDNA was analyzed. The phenol-sulfuric acid method was used to measure the EPSs concentration. Cell viability was tested by the MTT assay to quantify the EPS cytotoxicity effect. Wounding healing, gelatin zymography, and ELISA were used to measure cell migration ability.Results: MD4 was identified as a Staphylococcus sp. with 99% similarity. The results of the phenol sulfuric acid method showed that the purified EPS concentration from MD4 was 28.068 μg/μL. There was a concentration-dependent cytotoxic effect of MD4-EPS on MC-4 cells, with IC50 values of 0.891 μg/μL.The results of the MTT assay showed that MD4-EPS effectively inhibited the migration of MC-4 cells as the concentration increased. The gelatin zymography analysis showed that the enzymatic activities of the migration-related MMP2 and MMP9 proteins decreased after EPS treatment. Furthermore, the protein secretion levels of MMP2 and MMP9 after MD4-EPS treatment were significantly lower than those in the control and PBS groups.Conclusions: Our results demonstrate that the EPSs derived from the MD4 endophyte of O. japonicus acted as natural antitumor agents to inhibit the migration of gastric cancer cells.

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TCF21 inhibits proliferation and chemoresistance through the AKT pathway in human gastric cancer

Cited by 9 publications

References 28 publications

Identification of potential diagnostic and therapeutic target genes for lung squamous cell carcinoma

Identification of potential diagnostic and therapeutic target genes for lung squamous cell carcinoma

Comprehensive Analysis of Epigenetic Alternations of MicroRNA and DNA Methylation Contribute to Gene Expression and Prognosis in Lung Squamous Cell Carcinoma

Exopolysaccharides from an Ophiopogon japonicus endophyte inhibit proliferation and migration in MC-4 human gastric cancer cells

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