TCA cycle signalling and the evolution of eukaryotes

Ryan, Dylan Gerard; Frezza, Christian; O’Neill, Luke A. J.

doi:10.1016/j.copbio.2020.09.014

Cited by 39 publications

(25 citation statements)

References 87 publications

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“…The GABA branch can provide nicotinamide adenine dinucleotide (NADH) and succinate to feed the tricarboxylic acid (TCA) cycle [ 52 ]. This process is then of primary importance because enhanced TCA cycle could provide more energy for growth and stress defense under unfavorable environmental conditions [ 55 ]. Current findings showed that the Spm application further significantly improved the drought-induced increases in Glu content, GABA content, and GAD activity ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass

Tan

Hassan

Peng

et al. 2022

IJMS

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Due to increased global warming and climate change, drought has become a serious threat to horticultural crop cultivation and management. The purpose of this study was to investigate the effect of spermine (Spm) pretreatment on metabolic alterations of polyamine (PAs), γ-aminobutyric acid (GABA), proline (Pro), and nitrogen associated with drought tolerance in creeping bentgrass (Agrostis stolonifera). The results showed that drought tolerance of creeping bentgrass could be significantly improved by the Spm pretreatment, as demonstrated by the maintenance of less chlorophyll loss and higher photosynthesis, gas exchange, water use efficiency, and cell membrane stability. The Spm pretreatment further increased drought-induced accumulation of endogenous PAs, putrescine, spermidine, and Spm, and also enhanced PAs metabolism through improving arginine decarboxylases, ornithine decarboxylase, S-adenosylmethionine decarboxylase, and polyamine oxidase activities during drought stress. In addition, the Spm application not only significantly improved endogenous GABA content, glutamate content, activities of glutamate decarboxylase and α-ketoglutarase, but also alleviated decline in nitrite nitrogen content, nitrate reductase, glutamine synthetase, glutamate synthetase, and GABA aminotransferase activities under drought stress. The Spm-pretreated creeping bentgrass exhibited significantly lower ammonia nitrogen content and nitrite reductase activity as well as higher glutamate dehydrogenase activity than non-pretreated plants in response to drought stress. These results indicated beneficial roles of the Spm on regulating GABA and nitrogen metabolism contributing towards better maintenance of Tricarboxylic acid (TCA) cycle in creeping bentgrass. Interestingly, the Spm-enhanced Pro metabolism rather than more Pro accumulation could be the key regulatory mechanism for drought tolerance in creeping bentgrass. Current findings provide a comprehensive understanding of PAs interaction with other metabolic pathways to regulate drought tolerance in grass species.

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Section: Discussionmentioning

confidence: 99%

Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass

Tan

Hassan

Peng

et al. 2022

IJMS

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“…On the other hand, all cells share a metabolic core (e.g. the TCA cycle), facilitating the compatibility of metabolic networks [35, 36, 37]. Additionally, syntrophies and mutualisms in prokaryotes are commonplace, highlighting the relatively high likelihood for metabolic compatibility between prokaryotic cells configured in extracellular arrangements [38].…”

Section: Discussionmentioning

confidence: 99%

Metabolic compatibility and the rarity of prokaryote endosymbioses

Libby

Kempes

Okie

2022

Preprint

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The evolution of the mitochondria was a significant event that gave rise to the eukaryotic lineage and most large complex life. Central to the origins of the mitochondria was an endosymbiosis between prokaryotes. Yet, despite the potential benefits that can stem from a prokaryotic endosymbiosis, their modern occurrence is exceptionally rare. While many factors may contribute to their rarity, we lack methods for estimating the extent to which they constrain the appearance of a prokaryotic endosymbiosis. Here, we address this knowledge gap by examining the role of metabolic compatibility between a prokaryotic host and endosymbiont. We use genome-scale metabolic models from three different databases (AGORA, KBase, and CarveMe) to assess the viability, fitness, and adaptability of potential prokaryotic endosymbioses. We find that while more than half of host-endosymbiont pairings are metabolically viable, the resulting endosymbioses have reduced growth rates compared to their ancestral metabolisms and are unlikely to gain mutations to overcome these fitness differences. In spite of these challenges, we do find that they may be more robust in the face of environmental perturbations at least in comparison with the ancestral host metabolism lineages. Our results provide a critical set of null models and expectations for understanding the forces that shape the structure of prokaryotic life.

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“…Analysis of the 20 THIOM-interacting proteins in oocytes suggests that they mainly play roles in citrate metabolic processes, oxidative stress and electron transport chain. For example, mitochondrial aconitase (ACO2) is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the citric acid cycle [ 42 ]. NDUFS1, 2, and 3 are major subunits of mitochondrial respiratory chain complex I [ 43 ], and the interactions of THIOM with NDUFS1, 2 and 3 imply a possible involvement of THIOM in maintaining the functional and structural integrity of complex I.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile

Zhang

Sun

et al. 2021

Acta Pharmacol Sin

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Mitochondria are essential organelles that provide energy for mammalian cells and participate in multiple functions, such as signal transduction, cellular differentiation, and regulation of apoptosis. Compared with the mitochondria in somatic cells, oocyte mitochondria have an additional level of importance since they are required for germ cell maturation, dysfunction in which can lead to severe inherited disorders. Thus, a systematic proteomic profile of oocyte mitochondria is urgently needed to support the basic and clinical research, but the acquisition of such a profile has been hindered by the rarity of oocyte samples and technical challenges associated with capturing mitochondrial proteins from live oocytes. Here, in this work, using proximity labeling proteomics, we established a mitochondria-specific ascorbate peroxidase (APEX2) reaction in live GV-stage mouse oocytes and identified a total of 158 proteins in oocyte mitochondria. This proteome includes intrinsic mitochondrial structural and functional components involved in processes associated with “cellular respiration”, “ATP metabolism”, “mitochondrial transport”, etc. In addition, mitochondrial proteome capture after oocyte exposure to the antitumor chemotherapeutic cisplatin revealed differential changes in the abundance of several oocyte-specific mitochondrial proteins. Our study provides the first description of a mammalian oocyte mitochondrial proteome of which we are aware, and further illustrates the dynamic shifts in protein abundance associated with chemotherapeutic agents.

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TCA cycle signalling and the evolution of eukaryotes

Cited by 39 publications

References 87 publications

Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass

Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass

Metabolic compatibility and the rarity of prokaryote endosymbioses

Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile

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