TBX2 Regulates Proliferation, Apoptosis, and Cholesterol Generation of Bovine Through Maintaining Mitochondrial Function and Autophagy in Bovine Cumulus Cell

Abstract

TBX2 is a member of T-box gene family whose members are highly conserved in evolution and encoding genes are involved in the regulation of developmental processes. The encoding genes play an important role in growth and development, but its regulatory effects in bovine cumulus cells are still unclear. In this study, the changes of cell physiological function were detected after TBX2 gene was knocked down. Compared with the control group, when TBX2 was inhibited, the levels of autophagy, apoptosis and reactive oxygen species in cumulus cells were increased, the proliferation, expansion ability and the amount of cholesterol secreted by cells were significantly decreased, and the cell cycle was also disrupted. The results showed that TBX2 could regulate the normal physiological activities of bovine cumulus cells.