TbpBY167A-based vaccine is safe in pregnant sows and induces high titers of maternal derived antibodies that reduce Glaesserella parasuis colonization in piglets

Dellagostin, Diego; Klein, Rafaela Luiza; Giacobbo, Igor; Guizzo, João Antônio; Dazzi, C; Prigol, Simone Ramos; Gutiérrez-Martín, César B.; Kreutz, Luiz Carlos; Schryvers, Anthony B.; Frandoloso, Rafael

doi:10.1016/j.vetmic.2022.109630

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Maternally derived antibodies may protect piglets from early colonization by G. parasuis , thus underlying the importance of vaccinating sows (3). Recent research by Dellagostin et al (4) has shown that sow vaccination with the TbpB Y167A subunit vaccine effectively reduces G. parasuis colonization in suckling piglets. However, as this passive immunity diminishes during lactation, the integration of active piglet immunization becomes essential for controlling the infection at weaning (3).…”

Section: Discussionmentioning

confidence: 99%

“…The transmission of G. parasuis is predominantly facilitated from sows to piglets (3), with subsequent dissemination among piglets during the nursery phase. While neonatal protection is initially provided by colostrum-deprived maternal antibodies (4), the decline of these antibodies allows the pathogen to potentially overcome innate immunity, infiltrate target tissues, and develop the disease. This challenge is further complicated by the fact that maternal immunity can interfere with active immunization of piglets (5), and the current serovar-specificity of commercially available vaccines (6,7), failing to provide a complete protection in weaning piglets.…”

Section: Introductionmentioning

confidence: 99%

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TbpB-based oral mucosal vaccine provides heterologous protection against Glässer’s disease caused by different serovars of Spanish field isolates ofGlaesserella parasuis

González-Fernández,

Mencía-Ares,

García-Iglesias

et al. 2024

Preprint

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Background: Glaesserella parasuis has a substantial impact on the pig production as the primary agent of Glasser disease, particularly affecting nursery and early fattening stages. Current prophylactic measures, mainly based in serovar-specific bacterins administered parenterally to sows, face limitations due to maternal immunity, which may interfere with the active immunization of piglets. The mucosal administration of TbpB-based subunit vaccines offers a promising approach to overcome these limitations for the control of the disease in weaning piglets. This study evaluates the immunogenicity and heterologous protection of the oral mucosal TbpBY167A subunit vaccine in colostrum-deprived piglets challenged with four G. parasuis clinical isolates belonging to different TbpB clusters and serovars (SVs) recovered from Spanish pig farms. Results: The mucosal administration of a two-dose TbpB-based vaccine induced a robust humoral immune response in immunized colostrum-deprived piglets, significantly increasing IgA (p < 0.01) and IgM (p < 0.01) concentration 15 days after the second dose. Subsequent infection challenge with four G. parasuis clinical isolates demonstrated heterologous protection, markedly improving survival rates (OR: 8.45; CI 95%: 4.97-14.36) and significantly reducing clinical signs and lesions, regardless of the G. parasuis TbpB cluster and serovar. The vaccine not only reduced G. parasuis colonization in the respiratory tract of immunized piglets (p < 0.0001), but also in systemic target tissues, such as the tarsus and carpus joints, liver, and brain (p < 0.05). Further immunohistochemical analysis in different lung locations revealed a significantly lower macrophage count in immunized piglets (p < 0.0001). Conclusions: Overall, this study demonstrates that the oral mucosal administration of the TbpBY167A subunits vaccine in piglets provides effective heterologous protection against different virulent European G. parasuis field isolates, significantly reducing bacterial colonization and dissemination. These facts position this TbpB-based vaccine as a leading candidate for a universal vaccine against Glasser disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TbpB-based oral mucosal vaccine provides heterologous protection against Glässer’s disease caused by different serovars of Spanish field isolates ofGlaesserella parasuis

González-Fernández,

Mencía-Ares,

García-Iglesias

et al. 2024

Preprint

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Brazilian Clinical Strains of Actinobacillus pleuropneumoniae and Pasteurella multocida: Capsular Diversity, Antimicrobial Susceptibility (In Vitro) and Proof of Concept for Prevention of Natural Colonization by Multi-Doses Protocol of Tildipirosin

Kuchiishi,

Ramos Prigol,

Bresolin

et al. 2023

Antibiotics

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One hundred Actinobacillus pleuropneumoniae (App) and sixty Pasteurella multocida subsp. multocida serogroup A (PmA) isolates were recovered from porcine pneumonic lungs collected from eight central or southern states of Brazil between 2014 and 2018 (App) or between 2017 and 2021 (PmA). A. pleuropneumoniae clinical isolates were typed by multiplex PCR and the most prevalent serovars were 8, 7 and 5 (43, 25% and 18%, respectively). In addition, three virulence genes were assessed in P. multocida isolates, all being positive to capA (PmA) and kmt1 genes, all negative to capD and toxA, and most of them (85%) negative to pfhA gene. The susceptibility of both pathogens to tildipirosin was investigated using a broth microdilution assay. The percentage of isolates susceptible to tildipirosin was 95% for App and 73.3% for PmA. The MIC50 values were 0.25 and 1 μg/mL and the MIC90 values were 4 and >64 μg/mL for App and PmA, respectively. Finally, a multiple-dose protocol of tildipirosin was tested in suckling piglets on a farm endemic for both pathogens. Tildipirosin was able to prevent the natural colonization of the tonsils by App and PmA and significantly (p < 0.0001) reduced the burden of Glaesserella parasuis in this tissue. In summary, our results demonstrate that: (i) tildipirosin can be included in the list of antibiotics to control outbreaks of lung disease caused by App regardless of the capsular type, and (ii) in the case of clinical strains of App and PmA that are sensitive to tildipirosin based on susceptibility testing, the use of this antibiotic in eradication programs for A. pleuropneumoniae and P. multocida can be strongly recommended.

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TbpBY167A-based vaccine is safe in pregnant sows and induces high titers of maternal derived antibodies that reduce Glaesserella parasuis colonization in piglets

Cited by 2 publications

References 19 publications

TbpB-based oral mucosal vaccine provides heterologous protection against Glässer’s disease caused by different serovars of Spanish field isolates ofGlaesserella parasuis

TbpB-based oral mucosal vaccine provides heterologous protection against Glässer’s disease caused by different serovars of Spanish field isolates ofGlaesserella parasuis

Brazilian Clinical Strains of Actinobacillus pleuropneumoniae and Pasteurella multocida: Capsular Diversity, Antimicrobial Susceptibility (In Vitro) and Proof of Concept for Prevention of Natural Colonization by Multi-Doses Protocol of Tildipirosin

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