“…The analysis was performed as recommended by Selliah, Eck ( 22 ). Briefly, a panel of 48 sera ( Table 1 , Specificity) from SPF pigs immunized with Glaesserella parasuis (bacterin prepared with SV7 ( 21 ), n=8), Mycoplasma hyopneumoniae (Safesui Mycoplasma, Ourofino, n=8), Pasteurella multocida A (Bacterin formulated with a Brazilian clinical strain of P. multocida A, pfhA + , Vaxxinova, n=8), Salmonella enterica , serovar Choleraesuis (Enterisol ® SC-54 vaccine, Boehringer Ingelheim, n=8), Clostridium perfringes type C and Escherichia coli (Porcilis ® Coliclos, MSD, n=8) and Porcine circovirus type 2 (Porcilis ® PCV ID, MSD, n=8) were assessed using the protocol described in subsection 2.8.…”