Tbe complete nudeotide sequences of the cloned hepatitis B vims DNA; subtype adr and adw

Abstract

The complete nucleotide sequences of two different subtypes (adr and adw) of hepatitis B virus (HBV) DNA cloned in E. coli were determined. The sequence of the viral genome of the adr clone was 3188 nucleotides long, and that of the adw clone was 3200 nucleotides long. The adr and adw clones differed from the reported cloned ayw HBV DNA (3182 nucleotides long) in 11.2% and 10.0% of nucleotides, respectively. Heterogeneity of the HBV genome in the clones with the same subtype was observed.

Increased risk of adefovir resistance in patients with lamivudine-resistant chronic hepatitis B after 48 weeks of adefovir dipivoxil monotherapy

“…The sequences of the forward primer, reverse primer, and TaqMan probe (PE Biosystems, Foster City, CA), respectively, were as follows: 5Ј-CTCCCCGTCTGTKCCT-TCTCATC-3Ј (HBVRT1F, nucleotides 1542-1564; K ϭ G or T), 5Ј-GGCGTTCACGGTGGTCTC-CATGC-3Ј (HBVRT1R, nucleotides 1625-1603), and 5Ј FAM-CCGTGTGCACTTCGCTTCACCTCTGC-TAMRA 3Ј (HBV1TAQ, nucleotides 1575-1600). Nucleotide sequence positions were numbered according to Ono et al 19 Standard plasmid DNA was purified by CsCl gradient ultracentrifugation and calibrated using the World Health Organization International Standard for HBV DNA (NIBSC, Potters Bar, UK). Tenfold, serially diluted HBV DNAs ranging from 2.4 ϫ 10 1 to 2.4 ϫ 10 10 IU/mL were used to generate a standard curve for each reaction.…”

Immunochemical structure of the carboxy-terminal part of hepatitis B e antigen: identification of internal and surface-exposed sequences

“…The MAbs were obtained by screening primary hybridomas for activity in the Enzygnost anti-HBe assay (Behringwerke). The MAbs HBeOT6P and HBeOT7Q were raised against recombinant HBcAg (rHBcAg, subtype adw; Ono et at., 1983). The primary hybridoma clones were screened for MAb production by enzyme immunoassays (EIAs) using recombinant HBeAg (rHBeAg; Biogen) or rttBcAg (Chiron Corporation, P. Sillekens et al, unpublished).…”

Secretion of Particles of Hepatitis B Surface Antigen from Insect Cells Using a Baculovirus Vector

“…A pUC8-based plasmid (pUC8-HBs) containing the entire HBsAg coding region of the adw strain of HBV was constructed from the 1400 bp BamHI fragment of HBV DN A ( Fig. 1 ; Ono et al, 1983). The fragment was isolated from the recombinant plasmid and digested with AccI.…”