Taz1p and Teb1p, two telobox proteins in Schizosaccharomyces pombe, recognize different telomere-related DNA sequences

Vassetzky, Nikita S.; Gaden, Florence; Brun, Catherine; Gasser, Susan M.; Gilson, Éric

doi:10.1093/nar/27.24.4687

Cited by 36 publications

(40 citation statements)

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“…Perhaps one of the most striking and intriguing findings in this study is that Taz1, a well-characterized DNAbinding protein highly specific to telomeric repeats (Cooper et al 1997;Vassetzky et al 1999;Spink et al 2000), accumulated at the fusion points of Type A circular chromosomes where there are no telomeric repeat. In budding yeast, it has been shown by ChIP analysis that the telomeric DNA-binding protein, Rap1, and its binding partners associate with subtelomeric regions in addition to telomeric repeats (Strahl-Bolsinger et al 1997;Lieb et al 2001;Smith et al 2003).…”

Section: Genetic Inheritance Of Taz1 At Subtelomeres In the Absence Omentioning

confidence: 67%

Stable inheritance of telomere chromatin structure and function in the absence of telomeric repeats

Sadaie¹,

Naito²,

Ishikawa³

2003

Genes Dev.

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It is generally believed that telomeric repeats are a necessary and sufficient cis-element for telomere function. Here we show that telomere structure and meiotic function are stably inherited in fission yeast circular chromosomes that have lost all telomeric repeats. We found that the telomeric repeat binding protein, Taz1, and the heterochromatin protein, Swi6, remain associated with subtelomeres in the absence of telomeric repeats. We also found that the fusion point of circular chromosomes that lack telomeric repeats associates with SPB (the yeast counterpart of the centrosome) in the premeiotic horsetail stage, similarly to wild-type telomeres. However, a taz1 + deletion/reintroduction experiment revealed that the maintenance of Taz1 binding and premeiotic function is achieved via different strategies. Taz1 is recruited to subtelomeres by an autonomous element present in subtelomeric DNA, thus in a genetic mechanism. In contrast, the premeiotic subtelomere-SPB association is maintained in an epigenetic manner. These results shed light on the previously unrecognized role played by the subtelomere and underscore the robust nature of the functional telomere complex that is maintained by both genetic and epigenetic mechanisms. Furthermore, we suggest that the establishment and the maintenance of the functional telomere complex are mechanistically distinguishable.[Keywords: Telomere; subtelomere; epigenetic inheritance; circular chromosome; meiosis; Taz1] Supplemental material is available at http://www.genesdev.org.

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Section: Genetic Inheritance Of Taz1 At Subtelomeres In the Absence Omentioning

confidence: 67%

Stable inheritance of telomere chromatin structure and function in the absence of telomeric repeats

Sadaie¹,

Naito²,

Ishikawa³

2003

Genes Dev.

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“…Because yeast Rap1p functions both as a structural component of yeast telomeres and a transcriptional regulator (11,12), the presence of Myb-like domain and the involvement of transcriptional regulation may represent universal characteristics of telomere-binding proteins. This idea is further supported by the fact that a S. pombe telomeric protein, Teb1p, may function as a general transcription factor (24). An analysis of Arabidopsis DNA sequences available in data bases revealed that the sequence AAACCCTAA, corresponding to 1.3 units of the plant telomeric repeat AAACCCT, is preferentially located in the 5Ј region of the genes (29).…”

Section: Discussionmentioning

confidence: 95%

“…Recently, it has been shown that the isolated Myb-like domain of TRF1 binds specifically and with significant affinity to telomeric DNA as a monomer (20). Although with less specificity than the full-length dimer, the isolated Myb-like domain of Taz1p was also capable of sequence-specific DNA binding (23,24). Taken together, these results indicate that the Myblike domain is indeed responsible for specific telomeric DNA recognition.…”

mentioning

confidence: 85%

“…Interestingly, whereas the DNA binding domain of the Myb proteins typically consists of three tandem repeats of the Myb motif (21), Rap1p contains two Myb-like domains that bind DNA in a tandem orientation (22). In con-trast, the DNA binding domains of TRF1, TRF2, and Taz1p contain only a single Myb-like domain at their C terminus (16,23,24). Recently, it has been shown that the isolated Myb-like domain of TRF1 binds specifically and with significant affinity to telomeric DNA as a monomer (20).…”

mentioning

confidence: 99%

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Sequence-specific DNA Recognition by the Myb-like Domain of Plant Telomeric Protein RTBP1

Kim

et al. 2000

Journal of Biological Chemistry

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We have identified a rice gene encoding a DNA-binding protein that specifically recognizes the telomeric repeat sequence TTTAGGG found in plants. This gene, which we refer to as RTBP1 (rice telomere-binding protein 1), encodes a polypeptide with a predicted molecular mass of 70 kDa. RTBP1 is ubiquitously expressed in various organs and binds DNA with two or more duplex TTTAGGG repeats. The predicted protein sequence includes a single domain at the C terminus with extensive homology to Myb-like DNA binding motif. The Myb-like domain of RTBP1 is very closely related to that of other telomere-binding proteins, including TRF1, TRF2, Taz1p, and Tbf1p, indicating that DNA-binding domains of telomere-binding proteins are well conserved among evolutionarily distant species. To obtain precise information on the sequence of the DNA binding site recognized by RTBP1, we analyzed the sequence-specific binding properties of the isolated Myb-like domain of RTBP1. The isolated Myb-like domain was capable of sequence-specific DNA binding as a homodimer. Gel retardation analysis with a series of mutated telomere probes revealed that the internal GGGTTT sequence in the two-telomere repeats is critical for binding of Myblike domain of RTBP1, which is consistent with the model of the TRF1⅐DNA complex showing that base-specific contacts are made within the sequence GGGTTA. To the best of our knowledge, RTBP1 is the first cloned gene in which the product is able to bind doublestranded telomeric DNA in plants. Because the Myb-like domain appears to be a significant motif for a large class of proteins that bind the duplex telomeric DNA, RTBP1 may play important roles in plant telomere function in vivo.

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“…Both factors bind in vitro to the duplex DNA repeats with high affinity 2 and binding does not require a DNA end. 3,4 However, very low affinity binding to single-stranded DNA has recently been demonstrated, 5 with a net preference for the G-strand. TRF1 and TRF2 are found to co-localise, in vivo, with telomeres of human metaphase chromosomes.…”

Section: Introductionmentioning

confidence: 99%

A human interstitial telomere associates in vivo with specific TRF2 and TIN2 proteins

et al. 2002

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Mammalian telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex which protects the chromosome ends. Human telomere function is known to require two TTAGGG repeat factors, TRF1 and TRF2, and several interacting proteins, but the mechanism by which the DNA/protein complex prevents end to end fusion in vivo has not been elucidated. In order to better understand the role of specific telomere-associated proteins in the organisation of chromosome ends, we have studied a patient with a rare chromosome rearrangement that has given rise to an interstitial telomere. Using specific antibodies and immuno-FISH on unfixed metaphase chromosomes, we show that the proteins TRF2 and TIN2 (TIN2 interacts with TRF1) co-localise with the interstitial TTAGGG repeats. Our results demonstrate, for the first time in humans, that TRF2 and TIN2 proteins associate with interstitial duplex TTAGGG repeats, in vivo. They confirm that double stranded-telomeric repeats, even when complexed with specific proteins, are not sufficient to create a functional telomere. Finally, they suggest a possible role for proteins in stabilising interstitial TTAGGG repeats.

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Taz1p and Teb1p, two telobox proteins in Schizosaccharomyces pombe, recognize different telomere-related DNA sequences

Cited by 36 publications

References 0 publications

Stable inheritance of telomere chromatin structure and function in the absence of telomeric repeats

Stable inheritance of telomere chromatin structure and function in the absence of telomeric repeats

Sequence-specific DNA Recognition by the Myb-like Domain of Plant Telomeric Protein RTBP1

A human interstitial telomere associates in vivo with specific TRF2 and TIN2 proteins

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