Taxus sp. cell, tissue and organ cultures as alternative sources for taxoids production: a literature survey

Jaziri, Mondher El; Zhiri, Abdesselam; Guo, Yanwen; Dupont, Jean-Pierre; Shimomura, Koichiro; Hamada, Hiroki; Vanhaelen, Maurice; Homès, Jacques

doi:10.1007/bf00039697

Cited by 51 publications

(22 citation statements)

References 76 publications

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“…However, taxol content is extremely low in the normal Taxus cell cultures. Since the synthesis of secondary metabolites is believed to be associated with the plant's defence reactions against fungal attack, elicitation by biotic elicitors from fungi might enhance taxol content in Taxus cells (Jaziri et al 1996, Yu et al 2001. Before elicitor-induced taxol biosynthesis, an oxidative stress was also observed .…”

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confidence: 97%

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Importance of glucose-6-phosphate dehydrogenase in taxol biosynthesis in Taxus chinensis cultures

Yu¹,

Chen²,

Yang³

et al. 2005

Biol. plant.

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The roles of glucose-6-phosphate dehydrogenase (G6PDH) in paclitaxel production were investigated in cell suspension cultures of Taxus chinensis. In the normal cultures, the trend of G6PDH activity was similar to that of cell growth. Addition of glutamate increased G6PDH activity, while dehydroepiandrosterone (DHEA) decreased G6PDH activity. In elicitor-treated cultures, cell growth was depressed, while G6PDH activity and taxol production were enhanced compared with the control. Glutamate recovered the depression of cell growth, and resulted in further increase in G6PDH activity and taxol production. Contrarily, DHEA exacerbated the depression of cell growth, and decreased G6PDH activity and taxol production induced by fungal elicítor. The results indicated that G6PDH played a critic role of taxol production by affecting cell viability.

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confidence: 97%

“…Taxus cell culture is a promising means to obtain taxol and related taxane compounds (Jaziri et al 1996). However, taxol content is extremely low in the normal Taxus cell cultures.…”

mentioning

confidence: 99%

Importance of glucose-6-phosphate dehydrogenase in taxol biosynthesis in Taxus chinensis cultures

Yu¹,

Chen²,

Yang³

et al. 2005

Biol. plant.

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“…La determinación de la estructura química se realizó en 1971 (Jaziri et al, 1996) a partir de los análisis mediante difracción por rayos X. Fue todo un reto para los científicos de la época; así como posteriormente lo fue también, su síntesis total publicada en 1994 (Guo, Kai, Jin, & Tang, 2006). El nombre común para este taxoide ahora es paclitaxel y por lo tanto taxol es una marca registrada.…”

Section: Características Químicas Del Taxolunclassified

“…The determination of the chemical structure was performed in 1971 (Jaziri et al, 1996) from analyzes by means of X-ray diffraction, which was a challenge for scientists of that time; and so was the total synthesis published in 1994 (Guo, Kai, Jin, & Tang, 2006). Now, the common name for this taxoid is paclitaxel and therefore taxol is a registered trademark.…”

Section: Chemical Properties Of Taxolmentioning

confidence: 99%

TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

Barrales-Cureño¹,

Soto-Hernández²

2012

Rev Cha Se Cie For y del Amb

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RESUMENEl árbol del tejo (Taxus spp.) es una gimnosperma dicotiledónea que pertenece a la familia Taxaceae; se distribuye en America, Europa y Asia. Taxus spp. contiene en la corteza y en las hojas, alcaloides diterpénicos conocidos como taxoides. Éstos se caracterizan químicamente por la presencia de un esqueleto de taxano y un anillo de oxetano, tal como el taxol, que es utilizado en el tratamiento del cáncer de ovario, seno, próstata y pulmón; enfermedad que actualmente es una de las principales causas de muerte a nivel mundial. El contenido de taxol es bajo en los árboles, por tanto, es necesario el uso de alternativas biotecnológicas como lo es el cultivo in vitro de tejidos vegetales para incrementar la cantidad de taxoides y preservar el recurso natural. En esta revisión científica se reportan las características químicas de los taxoides, el mecanismo de acción biológico y semisíntesis orgánica del taxol, se detalla también la ruta biosintética de los principales taxoides, así como las principales enzimas involucradas para la obtención de éstos a nivel bioquímico y, finalmente, se indica de manera general la producción de taxoides en cultivos in vitro. ABSTRACTThe yew tree (Taxus spp.) is a dicot gymnosperm belonging to the Taxaceae family and it can be found in America, Europe and Asia. These trees have in their bark and leaves diterpenic alkaloids known as taxoids, which are chemically characterized by the presence of a taxane skeleton and an oxetane ring such as taxol, which is used in the treatment of ovarian, breast, prostate and lung cancer; disease that is currently one of the leading causes of death worldwide. Taxol content is low in trees, so it is necessary to use biotechnology alternatives such as in vitro cultivation of plant tissue to increase the amount of taxoids and preserve the natural resource. The chemical characteristics of taxoids, the mechanism of the biological action and the semi-synthetic organic of taxol were reported in this scientific review; as well as, the biosynthetic pathway of major taxoids and the main enzymes involved in obtaining taxoids at biochemical level and, finally the production of taxoids in vitro cultures is indicated.

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“…The last years numerous publications reported on the production of taxanes in plant tissue culture [2][3][4][5][6][7][8][9][10][11][12][13][14]. A recent literature survey reviews the methods used, the multiple factors and the feasibility of the in vitro production of taxanes [15]. In all these cases analysis was made by HPLC except from the report of Zhiri et al, where ELISA was used as the analytical method [12].…”

Section: Introductionmentioning

confidence: 98%

Application of SPE for the HPLC analysis of taxanes fromTaxus cell cultures

et al. 1998

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SummarySolid phase extraction (SPE) methods are studied for the sample pretreatment for HPLC analysis of Taxus cell suspension cultures. Various types of SPE materials were tested for the extraction of both the taxane standards and samples of various origin. Comparison between the different cartridges and the different elution solvents are made in terms of extraction recovery and sample clean-up. Selective elution of the taxanes is achieved by a gradient elution scheme.

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Taxus sp. cell, tissue and organ cultures as alternative sources for taxoids production: a literature survey

Cited by 51 publications

References 76 publications

Importance of glucose-6-phosphate dehydrogenase in taxol biosynthesis in Taxus chinensis cultures

Importance of glucose-6-phosphate dehydrogenase in taxol biosynthesis in Taxus chinensis cultures

TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

Application of SPE for the HPLC analysis of taxanes fromTaxus cell cultures

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