Taxonomy of Potyviruses Infecting Maize, Sorghum, and Sugarcane in Australia and the United States as Determined by Reactivities of Polyclonal Antibodies Directed towards Virus-Specific N-Termini of Coat Proteins

Amino Acid Sequence Homology of Coat Proteins as a Basis for Identification and Classification of the Potyvirus Group

“…Antibodies that did not bind to the column were found to recognize the homologous viruses and their strains. In contrast, the original unfractionated antisera contained antibodies to conserved core regions of the coat proteins and reacted with several distinct potyviruses (Shukla et al, 1988a(Shukla et al, , 1989. The results clearly demonstrated that virus-specific polyclonal antisera can be readily obtained by this method.…”

“…Recent results on potyvirus serology from our laboratory suggest that such an exercise is possible (Shukla et al, 1988a(Shukla et al, , d, 1989. First, the sequence data show that the N terminus is the only large region in the entire coat protein of a potyvirus that is virus-specific.…”

“…Members of several plant virus groups, tobamoviruses (Gibbs, 1977) and tymoviruses (Koenig & Lesemann, 1979) in particular, can be accurately classified on the basis of their serological properties. Although serology has so far failed to provide a useful taxonomy for the potyvirus group (Hollings & Brunt, 1981a;Francki et al, 1985), we have recently shown that coat protein sequence data, in combination with information on the nature of potyvirus particle assembly (Shukla et al, 1988d), can be used to develop rationally designed, simple serological techniques (Shukla et al, 1988a(Shukla et al, , 1989) that appear to be more useful and more easily applied than other properties for potyvirus identification and classification. Such technology was employed recently to show that 17 potyvirus strains infecting Poaceae were not all closely related strains of SCMV as previously believed but were forms of four distinct potyviruses namely SCMV, JGMV, maize dwarf mosaic virus and sorghum mosaic virus (Shukla et al, 1989).…”

Reactions and virus titres of differential sorghum inbred lines mechanically inoculated with an indian isolate of sorghum red stripe potyvirus (SRSV-Ind)

“…1989). Nevertheless, SRSV-lnd shares common epitopes with the strains of sorghum mosaic potyvirus (SrMV-H and M) (Mall, 1995;Giorda et al, 1986;Shukla et al, 1989). The SRSV-Ind is however, distinct from SrMV which causes mosaic and temperature independent red leaf but not necrotic red stripe symptoms in sorghum (Giorda et a/,, 1986).…”

A classification of the tobamoviruses based on comparisons among their 126K proteins

“…In the absence of data on nucleic acid sequences, studies on taxonomic relationships and differentiation among the members of individual groups of plant viruses have been based largely on comparisons among their coat proteins, using as data amino acid sequences (Gibbs, 1980;Shukla & Ward, 1988), amino acid composition (Fauquet et al, 1986a, b;Gibbs et al, 1982;Paul et aL, 1980;Tremaine & Argyle, 1970) or serological relatedness (Barnett et al, 1987;Francki et al, 1986;Shukla et al, 1989). However, the coat protein is encoded by a small percentage of the viral genome and the greater part is ignored in these types of comparisons.…”