Taxonomy of non H2-Lithotrophic, Oxalate-Oxidizing Bacteria Related to Alcaligenes eutrophus

Jenni, B; Realini, Laurence; Aragno, Michel; Tamer, A. Ü.

doi:10.1016/s0723-2020(88)80026-2

Cited by 36 publications

(31 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, the use of primers NH2-3 and HOM under various PCR conditions did not allow amplification of a band with the expected size from the DNA of strains JMP222 and JMP289. These observations indicated considerable sequence divergence between the maleylacetate reductase gene of R. eutropha 335 T and those of strains JMP222 and JMP289, and are in concordance with the report of Jenni et al (1988), who by DNA-DNA hybridization experiments found that strain JMP134, and consequently its derivatives JMP222 and JMP289, are too dissimilar to the type strain 335 T to be included in the species R. eutropha (formerly Alcaligenes eutrophus).…”

Section: Resultssupporting

confidence: 91%

Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate

et al. 2004

View full text Add to dashboard Cite

A gene cluster containing a gene for maleylacetate reductase (EC 1.3.1.32) was cloned from Ralstonia eutropha 335 T (DSM 531 T ), which is able to utilize 4-fluorobenzoate as sole carbon source. Sequencing of this gene cluster showed that the R. eutropha 335 T maleylacetate reductase gene, macA, is part of a novel gene cluster, which is not related to the known maleylacetate-reductase-encoding gene clusters. It otherwise comprises a gene for a hypothetical membrane transport protein, macB, possibly co-transcribed with macA, and a presumed regulatory gene, macR, which is divergently transcribed from macBA. MacA was found to be most closely related to TftE, the maleylacetate reductase from Burkholderia cepacia AC1100 (62 % identical positions) and to a presumed maleylacetate reductase from a dinitrotoluene catabolic gene cluster from B. cepacia R34 (61 % identical positions). By expressing macA in Escherichia coli, it was confirmed that macA encodes a functional maleylacetate reductase. Purification of maleylacetate reductase from 4-fluorobenzoate-grown R. eutropha 335 T cells allowed determination of the N-terminal sequence of the purified protein, which was shown to be identical to that predicted from the cloned macA gene, thus proving that the gene is, in fact, recruited for growth of R. eutropha 335 T with this substrate. INTRODUCTIONMaleylacetate reductases (EC 1.3.1.32) play a crucial role in the aerobic microbial degradation of aromatic compounds. They catalyse the NADH-or NADPH-dependent reduction of maleylacetate or 2-chloromaleylacetate to 3-oxoadipate ( Fig. 1) or of substituted maleylacetates to substituted 3-oxoadipates. In fungi, maleylacetate reductases contribute to the catabolism of very common substrates, such as tyrosine, gentisate, benzoate, 4-hydroxybenzoate, protocatechuate, vanillate, resorcinol and phenol (Karasevich & Ivoilov, 1977;Buswell & Eriksson, 1979;Gaal & Neujahr, 1979;Sparnins et al., 1979;Anderson & Dagley, 1980;Jones et al., 1995). For bacteria, it has been shown that maleylacetate reductases are involved in the degradation of resorcinol and 2,4-dihydroxybenzoate via hydroxyquinol (Larway & Evans, 1965;Chapman & Ribbons, 1976;Stolz & Knackmuss, 1993). Other substrates whose catabolic routes comprise this activity have a more complex structure, such as 2-hydroxydibenzo-p-dioxin and 3-hydroxydibenzofuran (Armengaud et al., 1999), or carry unusual substituents, such as nitro or sulfo groups (Spain & Gibson, 1991;Feigel & Knackmuss, 1993;Jain et al., 1994;Rani & Lalithakumari, 1994), fluorine or chlorine atoms (Duxbury et al., 1970; Schlömann et al., 1990a;Latus et al., 1995;Zaborina et al., 1995;Daubaras et al., 1996;Miyauchi et al., 1999).The maleylacetate reductase genes characterized so far tend to belong to specialized gene clusters for the degradation of aromatic compounds. Thus, 3-hydroxydibenzofuran and 2-hydroxydibenzo-p-dioxin degradation via hydroxyquinol appears to be encoded by a specialized gene cluster comprising most of the genes for the pathway including a maleylac...

show abstract

Section: Resultssupporting

confidence: 91%

Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate

et al. 2004

View full text Add to dashboard Cite

show abstract

“…All other strains were grown lithoautotrophically with H2 and CO2 as energy and carbon source in mineral medium supplemented with biotin (Jenni et al , 1988. Cells were resuspended in API-LRA medium (API code 88803) in order to inoculate API galenes 50CH, 50AO and 50AA (API codes 50CH-5030, 50AO-88801, 50AA-88802, API-bioMerieux SA, Geneva, Switzerland).…”

Section: Cultivation Conditions For Phenotypical Tests and Data Procementioning

confidence: 99%

“…The phenotypic data were treated by numerical analysis (Jenni et al , 1988. The Simple Matching coefficient (SsM; Sokal and Michener 1958), which includes both positive and negative matches, and the Jaccard coefficient (Sj; Jaccard 1908; Sheath 1957a) which does not take negative matches into account, were calculated.…”

Section: Cultivation Conditions For Phenotypical Tests and Data Procementioning

confidence: 99%

“…This indicates that the API substrate assimilation tests are applicable for the classification of new strains of the genus Xanthobacter. The same method was applied successfully for the taxonomy of Alcaligenes eutrophus (Jenni et al 1988). A limitation of this method (also noticed by Jenni et al 1988) is that negative growth with a few substrates in API galeries turns out to be positive when tested in aerated liquid cultures 9 Examples of this with strain 25a are acetate, glycerol, gluconate, which did not support grow in API tests but the organism clearly was able to assimilate these substrates in liquid cultures (see above).…”

Section: Api Analysis Of Strains H4-14 and 25amentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Xanthobacter strains H4-14 and 25a and enzyme profiles after growth under autotrophic and heterotrophic conditions

et al. 1990

Self Cite

View full text Add to dashboard Cite

“…LMG 1197; 3, strain 1263a T ; 4, C. oxalaticus; 5, C. basilensis DSM 11853 T ; 6, C. necator JCM 11282; 7, C. taiwanensis; 8, C. campinensis; 9, C. metallidurans; 10, C. pauculus; 11, C. gilardii; 12, C. respiraculi. Data are consolidated from our experiments and Goris et al (2001), Chen et al (2001), Vandamme et al (1999), Coenye et al (1999Coenye et al ( , 2003b, Jenni et al (1988) and Sahin et al (2000). +, Positive; 2, negative; d, variable result within the species; NA, no data available.…”

mentioning

confidence: 93%

Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines

Sato

Nishihara

Yoshida

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Taxonomic studies were performed on ten hydrogen-oxidizing, facultatively chemolithotrophic bacteria that were isolated from volcanic mudflow deposits derived from the eruption of Mt. Pinatubo in the Philippines in 1991. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the genus Cupriavidus of the Betaproteobacteria; sequence similarity values with their nearest phylogenetic neighbour, Cupriavidus basilensis, were 97.1–98.3 %. In addition to phylogenetic analysis, results of whole-cell protein profiles and biochemical tests revealed that these strains were members of two distinct species. DNA–DNA hybridizations and whole-cell protein profiles enabled these isolates to be differentiated from related Cupriavidus species with validly published names. The isolates were aerobic, Gram-negative, non-sporulating, peritrichously flagellated rods. Their G+C contents ranged from 65.2 to 65.9 mol% and their major isoprenoid quinone was ubiquinone Q-8. On the basis of these results, two novel species are proposed, Cupriavidus pinatubonensis sp. nov. [nine strains, with 1245T (=CIP 108725T=PNCM 10346T) as the type strain] and Cupriavidus laharis sp. nov. [one strain, the type strain 1263aT (=CIP 108726T=PNCM 10347T)]. It is also suggested that Ralstonia sp. LMG 1197 (=JMP 134) should be included in the species C. pinatubonensis.

show abstract

Taxonomy of non H2-Lithotrophic, Oxalate-Oxidizing Bacteria Related to Alcaligenes eutrophus

Cited by 36 publications

References 30 publications

Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate

Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate

Characterization of Xanthobacter strains H4-14 and 25a and enzyme profiles after growth under autotrophic and heterotrophic conditions

Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines

Contact Info

Product

Resources

About