“…The phylogenetic analysis results indicated the ITS-5.8S rDNA of endophyte PC27-5 and most strains of C. velutina shared over 99% identity and were within the minimal clade. Overall, most features of C. velutina PC27-5 were consistent with those of authentic C. velutina described in the literature (Davey et al, 2010;Chang and Wang, 2011). The genus Coniochaeta has been isolated in different environments.…”
Section: Resultssupporting
confidence: 78%
“…3B). The cultural and morphological data were strongly supportive of the molecular data, allowing the identification of PC27-5 as C. velutina (Davey et al, 2010;Chang and Wang, 2011). …”
Section: Phylogenetics Analysis Of 18s Rdna and Its-58s Rdnamentioning
confidence: 54%
“…One valuable, biologically-active fungus, identified as Coniochaeta velutina (PC27-5), was isolated from a healthy Western hemlock (Tsuga heterophylla) which was collected in this area. Most isolates of the genus Coniochaeta have been reported as opportunistic pathogens isolated from dung, necrotic wood, soil, and plant surfaces (Damm et al, 2010;Chang and Wang, 2011). Recently, a species of Coniochaeta with some antimicrobial properties was isolated from a rainforest in Thailand (Kokaew et al, 2011).…”
An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 μg/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non-biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol--anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.
“…The phylogenetic analysis results indicated the ITS-5.8S rDNA of endophyte PC27-5 and most strains of C. velutina shared over 99% identity and were within the minimal clade. Overall, most features of C. velutina PC27-5 were consistent with those of authentic C. velutina described in the literature (Davey et al, 2010;Chang and Wang, 2011). The genus Coniochaeta has been isolated in different environments.…”
Section: Resultssupporting
confidence: 78%
“…3B). The cultural and morphological data were strongly supportive of the molecular data, allowing the identification of PC27-5 as C. velutina (Davey et al, 2010;Chang and Wang, 2011). …”
Section: Phylogenetics Analysis Of 18s Rdna and Its-58s Rdnamentioning
confidence: 54%
“…One valuable, biologically-active fungus, identified as Coniochaeta velutina (PC27-5), was isolated from a healthy Western hemlock (Tsuga heterophylla) which was collected in this area. Most isolates of the genus Coniochaeta have been reported as opportunistic pathogens isolated from dung, necrotic wood, soil, and plant surfaces (Damm et al, 2010;Chang and Wang, 2011). Recently, a species of Coniochaeta with some antimicrobial properties was isolated from a rainforest in Thailand (Kokaew et al, 2011).…”
An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS-5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 μg/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non-biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol--anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.
“…These morphological characters as well as ecological characteristics of our collections fit the diagnosis of C. vagans (Doveri, 2004;Bell, 2005;Asgari et al, 2007;Chang, Wang, 2011). Additionally, in our specimens asci are mostly eight-spored; however, occasionally we observed six-or even four-spored asci, with clearly visible two, or respectively, four degenerated ascospores (Fig.…”
Section: Methodssupporting
confidence: 82%
“…1, e). In Ukrainian specimen, ascospores are slightly smaller than those indicated for C. leucoplaca by other authors (Doveri, 2004;Bell, 2005;Asgari et al, 2007;Chang & Wang, 2011) Asci eight-spored, cylindrical, with a short stipe, 87-95 × 7.7-8.5 μm; unitunicate, slightly narrowed and flattened at the apex, non-amyloid; apical apparatus well developed and easily observable. Ascospores uniseriate, one-celled, smooth, thick-walled, dark-brown, 7.5-8.5 × 5.2-6.5 × 3.9-4.5 μm, broadly ellipsoidal in frontal view (Q = 1.26-1.53, Q av = 1.34), narrowly ellipsoidal in side view (Q = 1.95-2.12, Q av = 2.05); germ slit runs around the whole circumference; gelatinous sheath hyaline, 1.7-2.2 μm wide.…”
Recent special studies on coprophilous ascomycetes (Golubtsova, 2008; Golubtsova et al., 2010) added eight new records for Ukraine. This article reports five species of coprophilous fungi of the genera Coniochaeta (Sacc.) Cooke and Sordaria Ces. & De Not., three previously unregistered in Ukraine species and two which are the second records for the country. Coniochaeta (Coniochaetaceae, Coniochaetales) is a cosmopolitan genus including lignicolous, terricolous and coprophilous species. The genus is characterized by small non-stromatic perithecioid ascomata bearing dark stiff setae clustered around the neck, or sometimes also scattered on the venter. Coniochaeta species have non-amyloid, unitunicate, short-stalked, cylindric asci and dark brown, one-celled, discoid ascospores with a germination slit along the narrow side and evanescent hyaline sheath surrounding ascospores. According to
During a study on the biodiversity of yeasts and yeast-like ascomycetes from wild plants in Iran, four strains of yeast-like filamentous fungi were isolated from a healthy plant of Euphorbia polycaulis in the Qom Province, Iran (IR. of). All four strains formed small hyaline one-celled conidia from integrated conidiogenous cells directly on hyphae and sometimes on discrete phialides, as well as by microcyclic conidiation. Two strains additionally produced conidia in conidiomata that open by rupture. The internal transcribed spacer (ITS) sequences suggested the placement of these strains in the genera Collophorina (Leotiomycetes) and Coniochaeta (Sordariomycetes), respectively. Blast search results on NCBI GenBank and phylogenetic analyses of ITS, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the translation elongation factor 1α (EF-1α) sequences, and the nuclear large subunit ribosomal gene (LSU), partial actin (ACT), and β-tubulin (TUB) sequences, respectively , revealed the isolates to belong to three new species, that are described here as Collophorina euphorbiae, Coniochaeta iranica, and C. euphorbiae. All three species are characterised by morphological, physiological, and molecular data.
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