Taxonomy and phylogeny of two species of the genus Deviata (Protista, Ciliophora) from China, with description of a new soil form, Deviata parabacilliformis sp. nov.

Abstract

Taxonomy and phylogeny of two species of the genus Deviata (Protista, Ciliophora) from China, with description of a new soil form, Deviata parabacilliformis sp. nov. The morphology and morphogenesis of a soil hypotrichous ciliate, Deviata parabacilliformis sp. nov., isolated from northern China, were investigated. D. parabacilliformis measures about 75-210¾25-60 mm in vivo, with an elongate and flexible body. It possesses one right marginal row, two to four left marginal rows and three dorsal kineties. The main morphogenetic features of D. parabacilliformis are: (i) the oral primordium originates de novo; (ii) anlage IV of the opisthe originates from parental frontoventral row V, anlage V originates de novo, and anlage VI forms from frontoventral row VI; and (iii) anlage I of the proter originates from the anterior portion of the parental paroral, anlage II originates from the buccal cirrus, anlage III originates from the parabuccal cirri, anlage IV originates from parental frontoventral row IV and anlage V forms from the anterior of parental frontoventral row VI. The morphology of an edaphic population of another species of the genus Deviata, Deviata bacilliformis (Gelei 1954) Eigner 1995, was also investigated. This work also provides the first record of SSU rRNA gene sequences for species of the genus Deviata. Molecular phylogenetic analysis suggests that Deviata is not monophyletic, and its position is poorly resolved due to weak phylogenetic signal of the 18S marker in the Stichotrichida. INTRODUCTIONThe ciliated protozoa are a species-rich and morphologically diverse group. Morphological identification is challenging for these organisms, especially for the highly complicated hypotrichs. This situation is being improved, however, as a result of staining techniques which can reveal the infraciliature and other characteristics precisely (Chen et al., 2013a, b; Jiang et al., 2013a, b;Li et al., 2013;Pan et al., 2013;Shao et al., 2013a; Fan et al., 2014;Gao & Katz, 2014;Huang et al., 2014;Jung et al., 2014).The genus Deviata was established by Eigner (1995) with Deviata abbrevescens as the type species. Diagnostic features of the genus include: non-dorsomarginalian hypotrichia with more than one long cirral row on right and left lateral sides of the body; one of the cirral rows right of the adoral zone terminates in the centre of the ventral surface (Berger, 2011); typical parental (old) cirral rows are absent; and multiple deviations within anlagen develop during morphogenesis (Eigner, 1995). According to Berger (2011), there are currently eight recognized species in the genus. Except for the type species D. abbrevescens, detailed morphogenetic processes are not known. Furthermore, there are currently no SSU rRNA gene sequence data for species of the genus Deviata and the phylogenetic position of the genus is uncertain (Berger, 2011).This paper describes the morphology and morphogenesis of a novel Deviata species, Deviata parabacilliformis Abbreviations: BI, Bayesian inference; ML, maximum-likelihood.The GenB...

Redescription, Morphogenesis, and Molecular Phylogeny of Pseudosincirra longicirrata nov. comb., With Establishment of a New Genus Pseudosincirra nov. gen. (Ciliophora, Hypotrichia)

“…The phylogenetic analyses showed that Pseudosincirra longicirrata nov. comb. groups with Deviata baciliformis (Gelei, 1954) Eigner, 1995 ; D. brasiliensis Siqueira-Castro et al, 2009 ; D. parabaciliformis Li et al, 2014 ; D. rositae Küppers et al, 2007 ; and Perisincirra paucicirrata Foissner et al, 2002 . The SSU rDNA sequence similarities of Pseudosincirra longicirrata nov. comb.…”

Redefinition of the hypotrichous ciliate Uncinata, with descriptions of the morphology and phylogeny of three urostylids (Protista, Ciliophora)

“…However, many nominal species are poorly known and their systematic positions are uncertain (Berger, 1999(Berger, , 2006Chen, Zhao, Ma, Warren, Shao & Huang, 2015;Chen, W., Chen, Li, Warren, & Lin, 2015;Kumar, Bharti, Marinsalti, Insom, & Terza, 2014;K€ uppers, 2014;Li et al, 2014;Shao, Chen, Pan, Warren, & Miao, 2014a;Singh & Kamra, 2015;Song, Warren, & Hu, 2009). This is mainly because early studies of hypotrichs tended only to provide descriptions of their living morphology, and to omit data on their infraciliature.…”

Morphology, Ontogeny, and Molecular Phylogeny of Two Freshwater Species of Deviata (Ciliophora, Hypotrichia) from Southern China

“…So far, nine species have been assigned to the genus (Berger ; Li et al. ): Deviata abbrevescens Eigner, ; Deviata bacilliformis (Gelei, 1954) Eigner, ; Deviata quadrinucleata (Dragesco ) Berger, ; Deviata brasiliensis Siqueira‐Castro et al., ; Deviata spirostoma (Alekperov, 1988) Berger, ; Deviata polycirrata Küppers and Claps, ; Deviata rositae Küppers et al., ; Deviata estevesi Paiva and Silva‐Neto, ; Deviata parabacilliformis Li et al., . Among them, only D. bacilliformis was redescribed (Berger ).…”

“…The SSU rRNA gene sequence data are available only for two species: D. bacilliformis and D. parabacilliformis, and not analyzed for the type species. Therefore, the phylogenetic position of the genus is basically not resolved (Li et al 2014).…”