Taxonomical classification and origin of Kamut® wheat

Michalcová, Veronika; Dušinský, Roman; Sabo, Miroslav; Beyroutiová, Maja Al; Hauptvogel, Pavol; Ivaničová, Zuzana; Švec, Miroslav

doi:10.1007/s00606-014-1001-4

Cited by 20 publications

(9 citation statements)

References 10 publications

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“…However, its dwarfing mechanism remains unknown. Additionally, Chinese Polish wheat exhibits low genetic similarity to T. durum , T. turgidum and common wheat 44 - 45 . Therefore, it is impossible to extrapolate the dwarfing mechanism of DPW from the transcriptomic and proteomic information of T. turgidum and common wheat 46 - 48 .…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

Wang¹,

Xiao²,

Wang³

et al. 2016

Int. J. Biol. Sci.

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The dwarfing mechanism of Rht-dp in dwarf Polish wheat (DPW) is unknown. Each internode of DPW was significantly shorter than it in high Polish wheat (HPW), and the dwarfism was insensitive to photoperiod, abscisic acid (ABA), gibberellin (GA), cytokinin (CK), auxin and brassinolide (BR). To understand the mechanism, three sets of transcripts, DPW, HPW, and a chimeric set (a combination of DPW and HPW), were constructed using RNA sequencing (RNA-Seq). Based on the chimeric transcripts, 2,446 proteins were identified using isobaric tags for relative and absolute quantification (iTRAQ). A total of 108 unigenes and 12 proteins were considered as dwarfism-related differentially expressed genes (DEGs) and differentially expressed proteins (DEPs), respectively. Among of these DEGs and DEPs, 6 DEGs and 6 DEPs were found to be involved in flavonoid and S-adenosyl-methionine (SAM) metabolisms; 5 DEGs and 3 DEPs were involved in cellulose metabolism, cell wall plasticity and cell expansion; 2 DEGs were auxin transporters; 2 DEPs were histones; 1 DEP was a peroxidase. These DEGs and DEPs reduced lignin and cellulose contents, increased flavonoid content, possibly decreased S-adenosyl-methionine (SAM) and polyamine contents and increased S-adenosyl-L-homocysteine hydrolase (SAHH) content in DPW stems, which could limit auxin transport and reduce extensibility of the cell wall, finally limited cell expansion (the cell size of DPW was significantly smaller than HPW cells) and caused dwarfism in DPW.

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Section: Introductionmentioning

confidence: 99%

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

Wang¹,

Xiao²,

Wang³

et al. 2016

Int. J. Biol. Sci.

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“…It also exhibits high antioxidant and mineral content (Ranhotra et al 1996). Kamut is tetraploid and has seeds with fewer allergen proteins and high levels of selenium, vitamins and minerals (Piergiovanni et al 2009;Michalcová et al 2014;Juhász et al 2015). Emmer and einkorn also exhibit different grain characteristics from common wheat (Shewry and Hey 2015).…”

Section: Introductionmentioning

confidence: 99%

Role of nitrogen-responsive plant-type phosphoenolpyruvate carboxylase in the accumulation of seed storage protein in ancient wheat (spelt and kamut)

Yamamoto

Kinoshita

Sugimoto

et al. 2017

Soil Science and Plant Nutrition

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Ancient wheat such as Triticum spelta L. and T. turanicum is a possible genetic material for improving wheat seed quality due to its grain characteristics, which differ from those of common wheat. Ancient wheat grains accumulate proteins with fewer allergens and higher levels of essential amino acids, antioxidants, minerals and vitamins. The identification of a genetic factor that controls seed protein content would be useful for breeding wheat varieties in accordance with various purposes. Previous studies implied that phosphoenolpyruvate carboxylase (PEPC) is involved in nitrogen accumulation in crop seeds. However, multiple types of PEPC genes are present in plant genomes, and it has still not been clarified yet which type of PEPC is relevant to seed nitrogen metabolism. In this study, to determine which PEPC is involved in seed protein accumulation in ancient wheat, we examined the effects of different levels of nitrogen application at the flowering stage on PEPC activity on developing seeds and on the protein content in mature seeds of three ancient wheat varieties. The responses of the PEPC activity and seed protein content were observed, but their patterns differed among the varieties. Among the experimental plots, PEPC activity and seed protein content showed a high correlation (r = 0.757, p value = 1.09E −3). Immunoblot analysis revealed that plant-type PEPC seemed to underlie the response of PEPC activity to nitrogen application, while no bacterial-type PEPC was detected by a peptide-antigen antibody. These results indicate that plant-type PEPC plays an important role in storage protein accumulation in ancient wheat seeds under nitrogen application at the flowering stage.

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“…Although proteomic changes in response to Cd or Zn have been successfully investigated using a proteomics approach (Kieffer et al, 2008 , 2009 ; Ahsan et al, 2009 ; Fukao et al, 2011 ; Lin and Arats, 2012 ; Schneider et al, 2013 ), the molecular mechanisms of Cd/Zn interactions are unknown, which limits our understanding of the interactions of Cd/Zn. Polish wheat (2 n = 4x = 28, AABB, Triticum polonicum L.), which has low genetic similarity with T. aestivum (Wang et al, 2013 ; Michalcová et al, 2014 ), accumulates high concentrations of Zn, Fe, and Cu and therefore has attracted the interest of producers and breeders (Wiwart et al, 2013 ). Meanwhile, dwarf polish wheat (DPW), collected from Tulufan, Xingjiang, China, shows high tolerance to Cd and Zn because its growth is not affected by the accumulation of high concentrations of these metals in seedlings (Wang X. et al, in press ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

Wang

et al. 2016

Front. Plant Sci.

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Cd and Zn have been shown to interact antagonistically or synergistically in various plants. In the present study of dwarf polish wheat (DPW)roots, Cd uptake was inhibited by Zn, and Zn uptake was inhibited by Cd, suggesting that Cd and Zn interact antagonistically in this plant. A study of proteomic changes showed that Cd, Zn, and Cd+Zn stresses altered the expression of 206, 303, and 190 proteins respectively. Among these, 53 proteins were altered significantly in response to all these stresses (Cd, Zn, and Cd+Zn), whereas 58, 131, and 47 proteins were altered in response to individual stresses (Cd, Zn, and Cd+Zn, respectively). Sixty-one differentially expressed proteins (DEPs) were induced in response to both Cd and Zn stresses; 33 proteins were induced in response to both Cd and Cd+Zn stresses; and 57 proteins were induced in response to both Zn and Cd+Zn stresses. These results indicate that Cd and Zn induce differential molecular responses, which result in differing interactions of Cd/Zn. A number of proteins that mainly participate in oxidation-reduction and GSH, SAM, and sucrose metabolisms were induced in response to Cd stress, but not Cd+Zn stress. This result indicates that these proteins participate in Zn inhibition of Cd uptake and ultimately cause Zn detoxification of Cd. Meanwhile, a number of proteins that mainly participate in sucrose and organic acid metabolisms and oxidation-reduction were induced in response to Zn stress but not Cd+Zn stress. This result indicates that these proteins participate in Cd inhibition of Zn uptake and ultimately cause the Cd detoxification of Zn. Other proteins induced in response to Cd, Zn, or Cd+Zn stress, participate in ribosome biogenesis, DNA metabolism, and protein folding/modification and may also participate in the differential defense mechanisms.

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Taxonomical classification and origin of Kamut® wheat

Cited by 20 publications

References 10 publications

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

Role of nitrogen-responsive plant-type phosphoenolpyruvate carboxylase in the accumulation of seed storage protein in ancient wheat (spelt and kamut)

Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.)

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