Taxonomic status of the vole in Daikoku Island, Hokkaido, Japan: examination based on morphology and genetics

Guia, Anna Pauline de; Saitoh, Takashi; Ishibashi, Yasuyuki; Abe, Hisashi

doi:10.3106/1348-6160(2007)32[33:tsotvi]2.0.co;2

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…Genomic DNA was extracted from samples (two toes per individual) using the conventional phenol‐chloroform method (Sambrook et al, 1989 ). The partial nucleotide sequences of the mtDNA control region were determined as described previously (de Guia et al, 2007 ), with some modifications. The most variable part (425 bp) was sequenced using primarily the internal primer HIP8, but also the primer Lpro, on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems) with the software Sequencing Analysis 3.4.1.…”

Section: Methodsmentioning

confidence: 99%

Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region

Ishibashi

2023

Ecology and Evolution

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Individual dispersal plays an important role in preserving genetic diversity in density‐fluctuating populations of arvicoline rodents. When habitats are fragmented and dispersal between habitats is severely constrained, genetic diversity can be lost. Here, I investigated whether genetic diversity in the gray‐sided vole Myodes rufocanus was preserved in an intensive farming region in Japan, where voles inhabited isolated windbreak forests along the borders of plowed lands. Genetic structure was examined in 673 vole samples (330 in spring and 343 in fall) collected at 34 windbreak forests located 0.35–20 km apart. A part of the control region (425 bp) of mitochondrial DNA (mtDNA) was sequenced in 673 voles, yielding 76 haplotypes. Genetic differentiation of maternally inherited mtDNA among trapping sites was markedly lower in males than in females in both seasons, indicating strong male‐biased dispersal. Genotypes at six microsatellite DNA loci were determined in 494 voles (245 in spring and 249 in fall) from 18 trapping sites, and loci harbored 16–24 alleles. The mean number of alleles per locus (allelic diversity) at trapping sites was positively correlated with the number of examined individuals (density) in both seasons, and the relationship was very similar to that of a previous study performed in much less fragmented populations. The genetic differentiation of microsatellite DNA among trapping sites decreased considerably from spring to fall. In a STRUCTURE analysis with a most probable cluster number of two, closer trapping sites showed more similar mean values of cluster admixture proportions. The present findings indicate that gene flow among isolated windbreak forests, which occurred mainly by dispersal of males, was not restrained in this intensive farming region. Furthermore, the results suggest that genetic diversity in the study population was preserved as well as in less fragmented populations.

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Section: Methodsmentioning

confidence: 99%

Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region

Ishibashi

2023

Ecology and Evolution

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Male-biased Dispersal Causes Intersexual Differences in the Subpopulation Structure of the Gray-sided Vole

Ishibashi

Zenitani

Saitoh

2013

Journal of Heredity

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The genetic structure of gray-sided voles was investigated at a spatial scale of 2 km using mtDNA sequences. The control region (674bp) of 162 voles was sequenced and 18 haplotypes were identified. Within 0.5-ha trapping plots (n = 8), the number of haplotypes and gene diversity was significantly greater in males than in females. The fixation index among plots for females (F GP = 0.241) was 3 times as large as that for males (0.075), implying male-biased dispersal. A simulation analysis showed that the observed genetic structure in males could be generated by modifying the observed haplotype distribution of females by adding the effects of local male dispersal. Half of the pairwise F GP (15/28) showed significant differentiation in females, whereas almost none (1/28) were significant in males. Isolation by distance was observed in females, whereas no clear spatial pattern was observed in males. Most pairwise F GP for females were not significant in the short- and intermediate-distance classes (≤1.0 km) as with those for males, whereas all showed significant differentiation in the long-distance class (>1.0 km) for females, but not for males. These findings indicate that the extent of subpopulations within which individuals interact differs between sexes.

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Morphological differentiation of Clethrionomys rufocanus Sundevall, 1846 and Clethrionomys rex Imaizumi, 1971 (Arvicolinae, Rodentia) in the zone of sympatry in the Far East

2012

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Taxonomic status of the vole in Daikoku Island, Hokkaido, Japan: examination based on morphology and genetics

Cited by 4 publications

References 17 publications

Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region

Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region

Male-biased Dispersal Causes Intersexual Differences in the Subpopulation Structure of the Gray-sided Vole

Morphological differentiation of Clethrionomys rufocanus Sundevall, 1846 and Clethrionomys rex Imaizumi, 1971 (Arvicolinae, Rodentia) in the zone of sympatry in the Far East

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