Taxonomic specificity of restriction-modification enzymes

Janulaitis, A.; Kazlauskienė, Rita; Lazarevičiuté, L.; Gilvonauskaĭte, R; Steponaviciene, D.; Jagelavičius, M.; Petrušyt, M.; Bitinait, J.; V≐ževičiuté, Z.; Kiudulien, E.; Butkus, Viktoras

doi:10.1016/0378-1119(88)90293-4

Cited by 17 publications

(14 citation statements)

References 6 publications

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“…The remaining strains await further investigation; they may produce either no restriction enzyme, or produce new Type I or other Types (II or III) of restriction enzymes. A previous survey of Type II restriction enzymes in E. coli revealed Type II isoschizomers at least in 25% (219/864) of the strains examined (14). …”

Section: Discussionmentioning

confidence: 93%

Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids

Ryu

Rowsell

2008

Nucleic Acids Research

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Although DNA-recognition sequences are among the most important characteristics of restriction enzymes and their corresponding methylases, determination of the recognition sequence of a Type-I restriction enzyme is a complicated procedure. To facilitate this process we have previously developed plasmid R-M tests and the computer program RM search. To specifically identify Type-I isoschizomers, we engineered a pUC19 derivative plasmid, pTypeI, which contains all of the 27 Type-I recognition sequences in a 248-bp DNA fragment. Furthermore, a series of 27 plasmids (designated ‘reference plasmids’), each containing a unique Type-I recognition sequence, were also constructed using pMECA, a derivative of pUC vectors. In this study, we tried those vectors on 108 clinical E. coli strains and found that 48 strains produced isoschizomers of Type I enzymes. A detailed study of 26 strains using these ‘reference plasmids’ revealed that they produce seven different isoschizomers of the prototypes: EcoAI, EcoBI, EcoKI, Eco377I, Eco646I, Eco777I and Eco826I. One strain EC1344 produces two Type I enzymes (EcoKI and Eco377I).

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Section: Discussionmentioning

confidence: 93%

Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids

Ryu

Rowsell

2008

Nucleic Acids Research

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“…All three bacteria have an optimum growth temperature below 30ºC, but their optimum restriction activity temperature is at 37ºC. Four neoschizomers have been previously described with the same 5´GAG↓CTC3´ cleavage sequence; two have been described in Escherichia coli (7), and the two others in Myxococcus xantus (14) and Enterobacter cloacae (10).…”

Section: Resultsmentioning

confidence: 99%

BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'

Chies¹,

Dias²,

Maia³

et al. 2006

Braz. J. Microbiol.

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A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG↓CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI. This is the first report of an isoschizomer and/or neoschizomer of the prototype SacI identified in the genus Bacillus.

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“…Janulaitis et al [18] tested many strains belonging to Enterobacteriaceae and discovered that, with one exception, all isolated enzymes recognize 6‐bp DNA sequences. The only strain recognizing a 4‐bp sequence shared only 10% DNA homology with the rest of the family members.…”

Section: Discussionmentioning

confidence: 99%

Mesophilic cyanobacteria producing thermophilic restriction endonucleases

Piechula

Waleron

Światek

et al. 2001

FEMS Microbiology Letters

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When searching for the site-specific endonucleases in several strains of Phormidium we made the following observations. Among the 16 strains that originated from 15 species of Phormidium, 12 produced one or more restriction enzymes, of which two produced the highly thermophilic restriction endonucleases PtaI and PpaAII with their optimum activity at 65-80 degrees C, which is far above the lethal temperature for the host microorganism (40 degrees C). These two temperature-resistant enzymes are isoschizomers of known BspMII and TaqI endonucleases, respectively. The presence of the thermophilic TaqI isoschizomer does not seem to play any role in the mesophilic host microorganism, which does not even contain an active cognate methyltransferase. Among the remaining 10 strains, six produced isoschizomers of endonucleases which we first described in cyanobacteria, namely: PfaAII (NdeI), PinBII and PtaI (BspMII), PlaAII (RsalI), PpaAII, PpeI (ApaI). Two enzymes, PauAII (AhaIII) and PfaAII (NdeI), belong to a group of a very rarely occurring isoschizomers. Out of 21 cyanobacterial endonucleases investigated by us, four were active in a wide range of temperatures (from 15 to 60 degrees C) which also extended the optimal growth temperature of the hosts. We assume that our observation on the presence of temperature-resistant restriction enzymes in mesophilic hosts supports the idea of horizontal gene transfer. Restriction modification systems may be an excellent tool for investigation of that phenomenon.

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Taxonomic specificity of restriction-modification enzymes

Cited by 17 publications

References 6 publications

Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids

Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids

BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'

Mesophilic cyanobacteria producing thermophilic restriction endonucleases

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