Taxonomic Relationships of Leafhopper Vectors of Plant Pathogens

Nielson, M. W.

doi:10.1016/b978-0-12-470280-6.50005-2

Cited by 56 publications

(32 citation statements)

References 55 publications

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“…Many phytoplasma strains are known to be transmitted by more than one vector, and several insect species are able to transmit the same phytoplasma strain (Nielson, 1979).…”

Section: Introductionmentioning

confidence: 99%

Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants

Alma

Bosco

Danielli

et al. 1997

Insect Molecular Biology

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A survey for the presence of aster yellows-related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested-PCR assays. Using a phytoplasma 16Srl group-specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma-free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows-related phytoplasmas in S. titanus.

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“…Many phytoplasma strains are known to be transmitted by more than one vector, and several insect species are able to transmit the same phytoplasma strain (Nielson, 1979).…”

Section: Introductionmentioning

confidence: 99%

Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants

Alma

Bosco

Danielli

et al. 1997

Insect Molecular Biology

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“…Some of the grassfeeding genera such as Cicadulina, Dalbulus, Baldulus, Nesoclutha and Macrosteles are vectors of plant pathogens (Nielson, 1979) and are consequently of importance to agriculture, particularly where they occur on cereals and sugarcane. However, Yamatotettix, found on sugarcane (Webb, 1986), and Balclutha, on grasses and rice (Knight, 1987), are not known to transmit plant pathogens.…”

Section: Introductionmentioning

confidence: 99%

Species recognition in Cicadulina leafhoppers (Hemiptera: Cicadellidae), vectors of pathogens of Gramineae

Webb

1987

Bull. Entomol. Res.

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“…Attachment of mycoplasmas to their Some Spiroplasma spp., which are helical members of the class Mollicutes, infect both plants and insects, like the phytoplasmas. The interaction between spiroplasmas, phytoplasmas and their insect vectors is highly specific (Nielson, 1979). For a plant pathogenic phytoplasma or spiroplasma to infect a leafhopper, several steps are involved.…”

Section: Introductionmentioning

confidence: 99%

An immunodominant membrane protein gene from the Western X-disease phytoplasma is distinct from those of other phytoplasmas The GenBank accession number for the sequence reported in this work is AF225904.

et al. 2001

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Membrane proteins mediate several important processes, including attachment, in several Mollicute species. Phytoplasmas are non-culturable plant pathogenic mollicutes that are transmitted in a specific manner by certain phloem-feeding insect vectors. Because it is likely that phytoplasma membrane proteins are involved with some aspect of the transmission process, their identification, isolation and characterization are important first steps in understanding phytoplasma transmission. A 32 kDa immunodominant protein (IDP) from the Western X-disease (WX) phytoplasma was purified from infected plants by immunoprecipitation using monoclonal antibodies, and two peptides from a tryptic digest were sequenced. PCR primers designed from these sequences amplified a 145 bp product which hybridized with WX-related phytoplasmas in Southern blots. This PCR product was used to identify a 25 kbp EcoRI-HindIII fragment that was cloned and sequenced. A complete 864 bp ORF (idpA) was identified for which the putative translation product contained both of the tryptic digest peptide sequences that were used to design the PCR primers. Analysis of the predicted IdpA sequence indicated two transmembrane domains but no cleavage point. The amino acid sequence had no significant homology with other known phytoplasma IDP genes. The idpA ORF was cloned into an Escherichia coli expression vector and a fusion protein of the predicted size was identified in Western blots using a WX-specific antiserum. A rabbit polyclonal antiserum was prepared to the purified expression protein and this reacted with both the E. coli-expressed and native WX phytoplasma proteins. This newly identified WX IDP (IdpA) is distinct from other known mollicute membrane proteins.

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Taxonomic Relationships of Leafhopper Vectors of Plant Pathogens

Cited by 56 publications

References 55 publications

Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants

Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants

Species recognition in Cicadulina leafhoppers (Hemiptera: Cicadellidae), vectors of pathogens of Gramineae

An immunodominant membrane protein gene from the Western X-disease phytoplasma is distinct from those of other phytoplasmas The GenBank accession number for the sequence reported in this work is AF225904.

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