Taxonomic relations between archaebacteria including 6 novel genera examined by cross hybridization of DNAs and 16S rRNAs

Tu, Jenn; Prangishvilli, David; Huber, Harald; Wildgruber, Gertrud; Zillig, Wolfram; Stetter, Karl O.

doi:10.1007/bf01810829

Cited by 86 publications

(28 citation statements)

References 13 publications

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“…Although these values are higher than those most used to divide genera of eubacteria (6, 24), they fall within the genus-species division value for the methanogenic bacteria proposed by Balch et al (1). This division is further supported by the DNA-rRNA hybridization data of Tu et al (29). The midpoint temperatures of the thermal melting curves for DNA-rRNA hybrids from that study were normalized by using a value of 20°C as the maximum heterologous AT'm(e) value (11).…”

Section: Methanogenium Marinigrismentioning

confidence: 84%

“…The electrophoretic patterns of ribosomal proteins suggest that there are important phylogenetic differences between phenotypically similar strains of Methanosarcina that may not be detected by rRNA oligonucleotide cataloging (5). Although the methanogenic bacteria have been previously studied by rRNA hybridization techniques, only one methylotrophic strain was included in that study (29). In this study the phylogenetic relationships of seven strains of methylotrophic methane-producing bacteria were determined by rRNA hybridization and DNA reassociation techniques, and taxonomic divisions are proposed.…”

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confidence: 99%

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Phylogenetic Relationships Among the Methylotrophic Methane-Producing Bacteria and Emendation of the Family Methanosarcinaceae

Sowers

Johnson

Ferry

1984

International Journal of Systematic Bacteriology

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The phylogenetic relationships among seven strains of methylotrophic methane-producing bacteria were determined by ribosomal ribonucleic acid hybridization and deoxyribonucleic acid homology techniques. The strains tested had deoxyribonucleic acid guanine-plus-cytosine contents of 39 to 43 mol% and represented a diversity of phenotypic characteristics. Our results indicate that these strains should be divided into six species within the genera Methanosarcina, Methanolobus, and Methanococcoides. The genus Methanosarcina includes Methanosarcina barkeri strains MS and 227, Methanosarcina sp. strain TM-1, Methanosarcina acetivorans strain C2A, and Methanosarcina matei strain S-6. The genus Methanolobus is represented by Methanolobus tindarius strain Tindari 3, and the genus Methanococcoides is represented by Methanococcoides methylutens strain TMA-10. Despite phenotypic similarities between Methanolobus tindarius and Methanococcoides methylutens, we propose that these species remain in separate genera based on differences in ribosomal ribonucleic acid homology and fractional differences in midpoint temperatures. The divisions indicated by deoxyribonucleic acid homology experiments complemented the ribosomal ribonucleic acid hybridization results. Phenotypic characteristics were consistent with these phylogenetic divisions; an apparent exception was cell wall composition, which is a conserved trait. Methanosarcina acetivorans had only a thin protein cell wall, but all other strans of Methanosarcina previously studied have been reported to have heteropolysaccharide cell walls. We present evidence which indicates that a protein component may be associated with the heteropolysaccharide cell wall of Methanosarcina barkeri strain 227.The methane-producing bacteria belong to the archaeobacteria, which, based on 16s oligonucleotide cataloging (6), are phylogenetically distinct from the eubacteria and eucaryotes. The most comprehensive phylogenetic study of the methanogenic bacteria was done by Balch et al. (l), who proposed the presently accepted divisions among this group. In that study, the Methanosarcinaceae, the only methanogenic family with methylotrophic species, was represented by three strains of one species. Since then, several methylotrophic methane-producing species have been described. These include Methanosarcina mazei (17), Methanothrix soehngenii (8), Methanolobus tindarius (15), Methanococcoides methylutens (26), and Methanosarcina acetivorans (2Sa). The taxonomic positions of only Methanothrix soehngenii (27) and Methanosarcina mazei (17) are phylogenetically classified by 16s ribosomal ribonucleic acid (rRNA) oligonucleotide cataloging; the other taxa are based on phenotypic characteristics and guanine-plus-cytosine (G + C) contents of deoxyribonucleic acids (DNAs). Another isolate, Methanosarcind sp. strain TM-1, also has been described ( 3 9 , but a specific epithet has not been proposed. The electrophoretic patterns of ribosomal proteins suggest that there are important phylogenetic differences between ...

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Section: Methanogenium Marinigrismentioning

confidence: 84%

mentioning

confidence: 99%

Phylogenetic Relationships Among the Methylotrophic Methane-Producing Bacteria and Emendation of the Family Methanosarcinaceae

Sowers

Johnson

Ferry

1984

International Journal of Systematic Bacteriology

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“…Based on 16S rRNA analysis Methanoplanus limicola DSM 2279 appears to be quite clearly located within the family Methanomicrobiaceae (Fig. 6), which has been described as a member of the family Methanoplanaceae (35,36,38). To describe genera which are phylogenetically and phenotypically coherent and distinct from related taxa we favor proposal two.…”

Section: S Rrna Oligonucleotide Signature Sequencementioning

confidence: 99%

Methanolacinia gen. nov., incorporating Methanomicrobium paynteri as Methanolacinia paynteri comb. nov.

Zellner

Messner

Kneifel³

et al. 1989

J. Gen. Appl. Microbiol.

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“…Methanogenic, halophilic, and sulfur-dependent archaebacteria have been phylogenetically classified (17). Halophilic archaebacteria (halophiles) have been subdivided into several major clusters by DNA-RNA hybridization (16), including strains that fall into nine groups on the basis of polar lipid pattern and rRNA analyses (15). These sophisticated molecular studies have not been paralleled by immunologic analysis.…”

mentioning

confidence: 99%

Immunologic distinctiveness of archaebacteria that grow in high salt

Macario¹,

König²,

Macario³

1986

J Bacteriol

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The antigenic fingerprints of eight halophilic archaebacteria representing the groups recently outlined by molecular and chemical analyses were determined with calibrated antibody probes. Comparison with the antigenic fingerprints of methanogens encompassing all described families and most genera demonstrated that these two archaebacterial groups are themselves antigenically coherent but immunologically distinct.

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Taxonomic relations between archaebacteria including 6 novel genera examined by cross hybridization of DNAs and 16S rRNAs

Cited by 86 publications

References 13 publications

Phylogenetic Relationships Among the Methylotrophic Methane-Producing Bacteria and Emendation of the Family Methanosarcinaceae

Phylogenetic Relationships Among the Methylotrophic Methane-Producing Bacteria and Emendation of the Family Methanosarcinaceae

Methanolacinia gen. nov., incorporating Methanomicrobium paynteri as Methanolacinia paynteri comb. nov.

Immunologic distinctiveness of archaebacteria that grow in high salt

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