Taxonomic reappraisal of <i>Gelidium coarctatum</i> (Gelidiales, Rhodophyta) and <i>Gelidium lineare sp. nov</i>. from the tropical western Atlantic

Iha, Cíntia; O'Shaughnessy, Kathryn A.; Guimarães, Sílvia M. P. B.; Oliveira, Mariana C.; Freshwater, D. Wilson

doi:10.2216/16-17.1

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…In their commentary paper, Hughey & Gabrielson 12 provided detailed laboratory protocols for the successful extraction of archival DNA. However, phylogenetic information recovered from archival specimens is typically limited due to the short length of sequences (about 200 base pairs [bp] or less) and to rbc L, a conserved marker 10 13 14 15 . Next-generation sequencing (NGS) is a cost-effective technique to derive sequence information, including complete organellar genomes, from the many valuable specimens stored in herbaria around the world 16 .…”

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confidence: 99%

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Mitogenomes from type specimens, a genotyping tool for morphologically simple species: ten genomes of agar-producing red algae

Boo

Hughey

Miller

et al. 2016

Sci Rep

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DNA sequences from type specimens provide independent, objective characters that enhance the value of type specimens and permit the correct application of species names to phylogenetic clades and specimens. We provide mitochondrial genomes (mitogenomes) from archival type specimens of ten species in agar-producing red algal genera Gelidium and Pterocladiella. The genomes contain 43–44 genes, ranging in size from 24,910 to 24,970 bp with highly conserved gene synteny. Low Ka/Ks ratios of apocytochrome b and cytochrome oxidase genes support their utility as markers. Phylogenies of mitogenomes and cox1+rbcL sequences clarified classification at the genus and species levels. Three species formerly in Gelidium and Pterocladia are transferred to Pterocladiella: P. media comb. nov., P. musciformis comb. nov., and P. luxurians comb. and stat. nov. Gelidium sinicola is merged with G. coulteri because they share identical cox1 and rbcL sequences. We describe a new species, Gelidium millariana sp. nov., previously identified as G. isabelae from Australia. We demonstrate that mitogenomes from type specimens provide a new tool for typifying species in the Gelidiales and that there is an urgent need for analyzing mitogenomes from type specimens of red algae and other morphologically simple organisms for insight into their nomenclature, taxonomy and evolution.

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“…Iha et al . 14 worked with sequences from the holotype of Gelidium coarctatum Kütz. (type locality: Pernambuco, Brazil) to reveal a misidentification, i.e., G .…”

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confidence: 99%

Mitogenomes from type specimens, a genotyping tool for morphologically simple species: ten genomes of agar-producing red algae

Boo

Hughey

Miller

et al. 2016

Sci Rep

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“…This result is unsurprising, as this region hosts a particularly high algal species diversity, including rare and endemic species (De Oliveira Filho 1969, Guimarães and Fujii 1998, Chen et al 2019. Moreover, its seaweed diversity is continuously growing as the use of molecular assisted identification is contributing to the discovery of new species (Nauer et al 2015, Iha et al 2016, Ximenes et al 2017, Brunelli et al 2019a, 2019b. The high diversity in this peculiar coast has been attributed to the substrate heterogeneity and availability, as well as the particular seawater temperature conditions because this region is a transition zone between tropical and subtropical regions and it is affected by upwelling events (Guimarães 2003).…”

Section: Discussionmentioning

confidence: 99%

<italic>Alsidium oliveiranum</italic> sp. nov. (Rhodomelaceae, Rhodophyta), an overlooked species from the southwestern Atlantic based on morphology and DNA sequence data

Guimarães¹,

Soares²,

Fujii³

et al. 2019

ALGAE

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The exploration of seaweed diversity in poorly studied habitats has often led to the discovery of new species. Sandcovered rocks are an example, as they received less attention than sand-free rocky intertidal habitats during seaweed diversity surveys in Brazil. In sand-covered rocks from Espírito Santo and Rio de Janeiro we found an alga whose morphology was unique among rhodomelacean species previously reported in Brazil. With the aim to clarify the taxonomic identity of this species we studied its morphology, as well as its phylogenetic relationships. Molecular analyses resolved this species in the genus Alsidium (tribe Alsidieae) and differed from sequenced congeners with divergences ≥2.5 and 4.2% in the rbcL and cox1 genes, respectively. Morphological characters were in agreement with the genus Alsidium, and differed from other species currently recognized in the genus. The species consisted of a basal crust and scarcely branched erect axes with seven pericentral cells covered by a continuous layer of cortical cells. Reproductive structures were formed on clusters of short determinate branches. Therefore, the new species A. oliveiranum is proposed based on morphological and molecular evidence. Our findings contribute to better understand the diversity of the tribe Alsidieae, which is particularly diverse in the Americas.

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“…DNA from type and old herbarium specimens from UC, MICH, WNC was extracted and amplified following procedures described by Boo, Hughey et al (2016). When large fragments of the analysed loci could not be amplified in these archival specimens, we were able to amplify and sequence 124 to 757 bp of rbcL and 230 bp of cox1 using primer pairs F145-R406, F577-R753, F753-R900, F993-R1144, and F1237-R1381 for rbcL (Boo et al 2015;Freshwater & Rueness 1994;Iha et al 2016;Kim et al 2010) and C622F and C880R for cox1 (Yang et al 2008). All rbcL and cox1 sequences generated in this study were deposited in GenBank (Table S1).…”

Section: Methodsmentioning

confidence: 99%

Gelidium adriaticum sp. nov. andGelidium carolinianum sp. nov. (Gelidiales, Rhodophyta) from the Mediterranean Sea

et al. 2019

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Identification of small Gelidium species based on morphology is difficult; as a consequence, the name Gelidium pusillum has been used for many small gelidiacean taxa throughout the world. Molecular-assisted identifications, however, are demonstrating that G. pusillum has a more restricted distribution than previously recognised. We used detailed morphological analyses combined with rbcL and cox1 sequence analyses to identify and assess the phylogenetic relationships of small Mediterranean Gelidium species. These analyses revealed the presence of two new species, G. adriaticum sp. nov. and G. carolinianum sp. nov. Gelidium adriaticum is a closely related sister species to G. pusillum, which was not found amongst our Mediterranean specimens. Gelidium carolinianum has been previously collected outside the Mediterranean, but it was misidentified as G. americanum. Morphological observations and sequence data generated from G. americanum types and other historical specimens clarified the status and distribution of this species. Phylogenetic analyses resolved G. carolinianum as a species in the Mediterranean and warm-temperate northwest Atlantic; whereas, G. americanum occurs in the Caribbean and tropical western Atlantic, and is sister to G. calidum from Brazil and closely related to G. crinale.

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Taxonomic reappraisal of Gelidium coarctatum (Gelidiales, Rhodophyta) and Gelidium lineare sp. nov. from the tropical western Atlantic

Cited by 10 publications

References 16 publications

Mitogenomes from type specimens, a genotyping tool for morphologically simple species: ten genomes of agar-producing red algae

Mitogenomes from type specimens, a genotyping tool for morphologically simple species: ten genomes of agar-producing red algae

<italic>Alsidium oliveiranum</italic> sp. nov. (Rhodomelaceae, Rhodophyta), an overlooked species from the southwestern Atlantic based on morphology and DNA sequence data

Gelidium adriaticum sp. nov. andGelidium carolinianum sp. nov. (Gelidiales, Rhodophyta) from the Mediterranean Sea

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