Taxonomic identification of rhizospheric actinobacteria isolated from Algerian semi-arid soil exhibiting antagonistic activities against plant fungal pathogens

Aouar, Lamia; Lerat, Sylvain; Ouffroukh, A.; Boulahrouf, Abderrahmane; Beaulieu, Carole

doi:10.1080/07060661.2012.681396

Cited by 22 publications

(19 citation statements)

References 62 publications

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“…genus. This result confirms the abundance of Streptomyces in telluric ecosystems compared to the other genera of actinobacteria (Kitouni et al, 2005;Aouar et al, 2012). There are several reports indicating that rhizosphere is a very selective environment and microorganisms growing in this environment has low diversity.…”

Section: Discussionsupporting

confidence: 84%

“…All of 12 isolates (100%) representatives of each group were active against the fungal phytopathogens tested. This percentage was higher than those reported by many authors studying the activity of soil actinomycetes (Aouar et al, 2012;Palaniyandi et al, 2013). Out of 12 distinct rhizospheric actinobacterial isolates, 3 of them namely PFK4, PFBOT7 and PFEL2 exhibited high antagonistic efficiency against P. myriotylum and other fungi and bacteria pathogens tested.…”

Section: Discussioncontrasting

confidence: 58%

“…Actinobacteria are saprophytic or symbiotic microorganisms widely associated with plants (Palaniyandi et al, 2013;Mouafo Tchinda et al, 2016). Although actinobacteria have been successfully isolated from epigeous plant tissues (Mouafo Tchinda et al, 2016), the rhizosphere represents the main reservoir for the search of such microorganisms (Aouar et al, 2012;Boudjeko et al, 2017). Some actinobacteria are plant growth promoting (PGP) and can promote growth directly by either facilitating resource acquisition (nitrogen, phosphorus and essential minerals), facilitating iron acquisition through siderophore production and/or modulating plant hormone levels (Schrey andTarkka 2008, Lugtengberg andKimilova 2009).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of antagonistic activities against Pythium myriotylum and plant growth promoting traits of Streptomyces isolated from Cocoyam (Xanthosoma sagittifolium (L.) Schott) rhizosphere

Kouomou¹,

Ewané²,

Lerat

et al. 2019

Aust J Crop Sci

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The focus of this study was to isolate, evaluate and characterize cocoyam rhizospheric actinobacteria collected from Mount Cameroon for their potential antagonistic activities against Pythium myriotylum, the causative agent of cocoyam root rot disease, and/or their ability to promote plant growth. Actinobacteria were isolated from soil samples by using serial dilution method. Dual culture assay was used to screen the isolates against fungal phytopathogens including P. myriotylum. The selected isolates were characterized morphologically, physiologically, biochemically and genetically. Furthermore, these isolates were subjected to in vitro production of various plant growth promoting (PGP) traits. Among the 287 isolates distributed into 12 distinct groups, PFK4, PFBOT7 and PFEL2 had high antagonistic effects against P. myriotylum and they were regarded as promising candidates for further study of their biocontrol ability. The identification of actinobacterial isolates was done using universal PCR of partial 16S rRNA sequences combined with bioinformatics and phylogenetic procedures. The results revealed that all these isolates belong to the genus Streptomyces spp. The isolate PFK4 showed 99% similarity with Streptomyces albulus while PFBOT7 showed 99% similarity with Streptomyces albus and PFEL2 showed 98% similarity with Streptomyces gandoceansis based on their 16S rRNA gene sequences. In this study for the first time, we report S. gandocaensis strain for the biological control of Pythium myriotylum. The role of these isolates in Plant Growth Promotion (PGP) by in vitro production of PGP traits such as phosphate solubilisation, siderophore, indole-3-acetic acid (IAA), ACC deaminase and cell wall degrading enzymes production were also evaluated and showed as potential PGP agents. Concurrent production of PGP substances coupled with broad spectrum of antifungal and antibacterial activities of these three strains suggest their potential use as promising biocontrol and PGP tools for the development of an alternative control method for cocoyam cultivation.

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Section: Discussionsupporting

confidence: 84%

Section: Discussioncontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

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Evaluation of antagonistic activities against Pythium myriotylum and plant growth promoting traits of Streptomyces isolated from Cocoyam (Xanthosoma sagittifolium (L.) Schott) rhizosphere

Kouomou¹,

Ewané²,

Lerat

et al. 2019

Aust J Crop Sci

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“…Tests for antimicrobial activities against fungi and oomycetes were performed on potato dextrose agar (PDA) plates at 28°C for 5 d. The antagonistic activities of JJY4 T against Aspergillus niger UdS-203, Botrytis cinerea B191, Fusarium oxysporum UdS-212, Phytophthora erythroseptica UdS-305, Phytophthora infestans UdS-302, P. megakarya Ngo13, Pythium aphanidermatum UdS-321, and Pythium myriotylum P60 were evaluated according to Aouar et al (4). Briefly, JJY4 T was streaked across the center of PDA plates and incubated at 25°C for 5 d. Two discs (7 mm in diameter) from an 8-d-old fungal culture were placed 1.5 cm from the edge of the plates.…”

Section: Methodsmentioning

confidence: 99%

“…Color was visually estimated by comparing the culture with chips from ISCC-NBS color charts (18) and melanin pigment production was evaluated based on growth on tyrosine (ISP-7) agar. The morphology of spore-bearing hyphae was assessed after 10–12 d of growth on starch-inorganic salt (ISP-4) agar using the cover-slip method (42) and by scanning electron microscopy as previously described (4). …”

Section: Methodsmentioning

confidence: 99%

<i>Streptomyces cameroonensis</i> sp. nov., a Geldanamycin Producer That Promotes <i>Theobroma cacao</i> Growth

Boudjeko

Tchinda

Zitouni

et al. 2017

Microbes and environments

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The taxonomy of an actinobacterial strain, designated JJY4T, was established using a polyphasic approach. JJY4T was isolated from the rhizosphere of Chromolaena odorata in Yaoundé (Cameroon) during a project for the selection of biological control agents. Strain JJY4T exhibited antimicrobial activities against bacteria, fungi, and oomycetes. Strain JJY4T also exhibited the traits of plant growth-promoting rhizobacteria such as the solubilization of inorganic phosphate, production of siderophores and indole-3-acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase activity. In planta assays performed on cocoa plantlets confirmed that strain JJY4T exhibited strong abilities to promote plant growth and protect against Phytophthora megakarya, the main causal agent of cocoa pod rot. The formation of rugose-ornamented spores in spiral spore chains by strain JJY4T is a typical feature of members found in the Streptomyces violaceusniger clade and, similar to some members of the clade, strain JJY4T produces geldanamycin. A phylogenetic analysis based on 16S rRNA gene sequences confirmed this classification and suggests that strain JJY4T be added to the subclade constituted of the type strains Streptomyces malaysiensis DSM 41697T and Streptomyces samsunensis DSM 42010T. However, DNA–DNA relatedness and physiological characteristics allowed for the differentiation of strain JJY4T from its closest phylogenetic relatives. Based on these results, strain JJY4T (=NRRL B-65369, =NBRC 112705) appears to represent a novel species in the S. violaceusniger clade for which the proposed name is Streptomyces cameroonensis sp. nov.

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An Overview on Nocardiopsis Species Originating From North African Biotopes as a Promising Source of Bioactive Compounds and In Silico Genome Mining Analysis of Three Sequenced Genomes

Ouchene,

Zaatout,

Suzuki

2024

Journal of Basic Microbiology

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Actinobacteria are renowned for their prolific production of diverse bioactive secondary metabolites. In recent years, there has been an increasing focus on exploring “rare” genera within this phylum for biodiscovery purposes, notably the Nocardiopsis genus, which will be the subject of the present study. Recognizing the absence of articles describing the research process of finding bioactive molecules from the genus Nocardiopsis in North African environments. We, therefore, present a historical overview of the discoveries of bioactive molecules of the genus Nocardiopsis originating from the region, highlighting their biological activities and associated reported molecules, providing a snapshot of the current state of the field, and offering insights into future opportunities and challenges for drug discovery. Additionally, we present a genome mining analysis of three genomes deposited in public databases that have been reported to be bioactive. A total of 36 biosynthetic gene clusters (BGCs) were identified, including those known to encode bioactive molecules. Notably, a substantial portion of the BGCs showed little to no similarity to those previously described, suggesting the possibility that the analyzed strains could be potential producers of new compounds. Further research on these genomes is essential to fully uncovering their biotechnological potential. Moving forward, we discuss the experimental designs adopted in the reported studies, as well as new avenues to guide the exploration of the Nocardiopsis genus in North Africa.

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Taxonomic identification of rhizospheric actinobacteria isolated from Algerian semi-arid soil exhibiting antagonistic activities against plant fungal pathogens

Cited by 22 publications

References 62 publications

Evaluation of antagonistic activities against Pythium myriotylum and plant growth promoting traits of Streptomyces isolated from Cocoyam (Xanthosoma sagittifolium (L.) Schott) rhizosphere

Evaluation of antagonistic activities against Pythium myriotylum and plant growth promoting traits of Streptomyces isolated from Cocoyam (Xanthosoma sagittifolium (L.) Schott) rhizosphere

<i>Streptomyces cameroonensis</i> sp. nov., a Geldanamycin Producer That Promotes <i>Theobroma cacao</i> Growth

An Overview on Nocardiopsis Species Originating From North African Biotopes as a Promising Source of Bioactive Compounds and In Silico Genome Mining Analysis of Three Sequenced Genomes

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