Taxonomic examination of Korean seagrasses 1 - Morphology and distribution of the genus Phyllospadix ( Zosteraceae )

Phylogenetic Analysis of Phyllospadix iwatensis Based on Nucleotide Sequences Encoding 18S rRNA and ITS-1

“…Phyllospadix, which is distributed along the coast of Korea and Japan, is a perennial seagrass belonging to the family Zosteraceae. Although the morphological charac-teristics and growth dynamics of Phyllospadix have been fairly extensively examined (Shin et al, 1993;Tomlinson, 1982;Park and Lee, 2009), little information is available on its detailed molecular characteristics and phylogenetic analysis. Ribosomal RNA has been used as one of the most common markers for analyzing the molecular phylogeny of macroalgae (Bird et al, 1992;Ragan et al, 1994), although its use has sometimes been known to be hindered by discrepancies due to the presence of duplicate copies.…”

“…Two species in the genus, P. iwatensis and P. japonica, are distributed along the coastline in Korea and Japan. Morphological differences between these two species include the apical shape of the leaf, the number of veins in the leaf, and the color of fibrous remains in the rhizome (Shin et al, 1993). P. iwatensis is characterized by a rounded apex, three and five veins in the apical and lower portions of the leaf, respectively, and brown fibrous remains in the rhizome (Kuo et al, 1990;Yabe et al, 1995).…”

“…P. iwatensis is characterized by a rounded apex, three and five veins in the apical and lower portions of the leaf, respectively, and brown fibrous remains in the rhizome (Kuo et al, 1990;Yabe et al, 1995). Although the morphological characteristics of P. iwatensis have been fairly well documented (Shin et al, 1993), little information has been available on its molecular markers and phylogenetic analysis. To perform phylogenetic analysis of Phyllospadix using molecular markers, nucleotide sequences of the regions encoding the 18s rRNA and ITS-1 of P. iwatensis were determined in this study.…”

Antimelanogenic effects of luteolin 7-sulfate isolated fromPhyllospadix iwatensisMakino

“…The P. iwatensis plant material was collected from the submerged coastal area of Uljin (37°01ʹ708ʹʹN, 129°24ʹ822ʹʹE), Korea in March 2015. Plant identity was confirmed by D.S.K., an experienced marine plant taxonomist, based on morphological characteristics such as rounded apex, five veins in the leaf and brown fibrous remains in the rhizome . The dried P. iwatensis sample (500 g) was cut and extracted with 80% aqueous ethanol (5 L) at room temperature for 5 days.…”

“…Plant identity was confirmed by D.S.K., an experienced marine plant taxonomist, based on morphological characteristics such as rounded apex, five veins in the leaf and brown fibrous remains in the rhizome. 26 The dried P. iwatensis sample (500 g) was cut and extracted with 80% aqueous ethanol (5 L) at room temperature for 5 days. The extract was subsequently evaporated under reduced pressure, and the crude extract was obtained at a yield of 12Á8% per dry weight of the original plant material.…”