Taxonomic characterization of two rubber degrading bacteria belonging to the species Gordonia polyisoprenivorans and analysis of hyper variable regions of 16S rDNA sequences

Abstract

Two cis‐1,4‐polyisoprene (isoprene rubber) degrading bacteria, strains VH2 and Y2K, were identified as strains of the species Gordonia polyisoprenivorans belonging to the Corynebacterineae, a suborder of the order Actinomycetales. Both showed characteristic growth and degradation of isoprene rubber as described previously for the type strain of G. polyisoprenivorans Kd2 (DSM 44302T). For strain VH2 the chemotaxonomic properties were investigated, and DNA–DNA hybridization experiments with the type strain revealed the affiliation to the species G. polyisoprenivorans. The comparison of the 16S rDNA sequences, and especially hyper variable regions of these, led to the classification of strain Y2K to the same species. At present, the species G. polyisoprenivorans comprises three different isolates which share the ability to degrade isoprene rubber potently but which were obtained from different geographic regions.

Biochemical and spectroscopic characterization of purified Latex Clearing Protein (Lcp) from newly isolated rubber degrading Rhodococcus rhodochrous strain RPK1 reveals novel properties of Lcp

“…lcp genes are frequently present in the genomes of Actinobacteria [ 22 ] and many rubber degrading species have been described for members from this group [ 3 – 5 , 23 , 31 , 32 ]. Most of the rubber degrading actinomycetes such as Streptomyces sp.…”

Complete Genome Sequence of Gordonia terrae 3612

“…are ubiquitous slightly acid-fast nocardioform bacteria classified within the order Actinomycetales ( 1 ). Gordonia species are important components of wastewater treatment systems ( 2 ) and are noted for their biodegradation abilities ( 3 – 5 ) and as opportunistic pathogens ( 6 – 8 ). Gordonia terrae 3612 is a type strain maintained by ATCC (ATCC 25594), NRRL (NRRL B-16283), and NBRC (NBRC 100016).…”

Investigation of the active biofilm communities on polypropylene filter media in a fixed biofilm reactor for wastewater treatment

“…Biological methods play a crucial role in wastewater treatment . Biofilm‐based technologies for the treatment of municipal and industrial wastewater were developed to overcome several disadvantages faced by conventional activated sludge systems and often produce higher effluent quality . The performance of biological wastewater treatment processes is determined by the activity of microorganisms .…”