Taxonomic assessment of Culicoides brunnicans , C. santonicus and C. vexans (Diptera: Ceratopogonidae) in France: Implications in systematics

Hadj-Henni, Leila; Meulemeester, Thibaut De; Mathieu, Bruno; Depaquit, Jérôme; Augot, Denis

doi:10.1016/j.meegid.2015.05.024

Cited by 11 publications

(10 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ribosomal DNA markers have been used to investigate phylogenies of closely related species (ITS1 and ITS2: [ 25 , 26 , 49 ]; 28S: [ 27 , 28 ]), interspecific genetic distances (ITS1 [ 43 , 47 ]) and population structure (ITS1: [ 53 ]) within Culicoides . The sequences obtained with ITS1 are generally of low quality [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of 42 species ofCulicoides(Diptera, Ceratopogonidae) from three continents

Augot¹,

Mathieu²,

Hadj-Henni³

et al. 2017

Parasite

View full text Add to dashboard Cite

The genus Culicoides includes vectors of important animal diseases such as bluetongue and Schmallenberg virus (BTV and SBV). This genus includes 1300 species classified in 32 subgenera and 38 unclassified species. However, the phylogenetic relationships between different subgenera of Culicoides have never been studied. Phylogenetic analyses of 42 species belonging to 12 subgenera and 8 ungrouped species of genus Culicoides from Ecuador, France, Gabon, Madagascar and Tunisia were carried out using two molecular markers (28S rDNA D1 and D2 domains and COI mtDNA). Sequences were subjected to non-probabilistic (maximum parsimony) and probabilistic (Bayesian inference (BI)) approaches. The subgenera Monoculicoides, Culicoides, Haematomyidium, Hoffmania, Remmia and Avaritia (including the main vectors of bluetongue disease) were monophyletic, whereas the subgenus Oecacta was paraphyletic. Our study validates the subgenus Remmia (= Schultzei group) as a valid subgenus, outside of the subgenus Oecacta. In Europe, Culicoides obsoletus, Culicoides scoticus and Culicoides chiopterus should be part of the Obsoletus complex whereas Culicoides dewulfi should be excluded from this complex. Our study suggests that the current Culicoides classification needs to be revisited with modern tools.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of 42 species ofCulicoides(Diptera, Ceratopogonidae) from three continents

Augot¹,

Mathieu²,

Hadj-Henni³

et al. 2017

Parasite

View full text Add to dashboard Cite

show abstract

“…For our study, left and right wings were used without any distinction because: (i) systematic selection of one side may bias the results in case of differential directional asymmetry among species [ 29 ]; (ii) comparison of wings from catalogs and original descriptions with status (left or right) are mostly unknown [ 16 ]; (iii) distribution and color spots on both wings are similar [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…An interactive identification key protocol for Culicoides has been previously developed to define the morphological descriptors required for accurate identification [ 11 ], where six taxonomists have validated the key, and its success rate ranged from 35.1% to 81.1% depending on Culicoides species concerned and users’ levels of expertise in Culicoides identification. Despite the results by our group and by others in the field, the current morphometric process for the identification of Culicoides relies on manual detection and identification of critical landmarks, which is a laborious process depending on the ability and expertise of a limited number of entomologists, and which can suffer from bias from one expert to another (for instance the number and position of landmarks varied from 10 to 14 in previously reported analysis) [ 12 – 16 ].…”

Section: Introductionmentioning

confidence: 95%

An approach to automatic classification of Culicoides species by learning the wing morphology

et al. 2020

View full text Add to dashboard Cite

Fast and accurate identification of biting midges is crucial in the study of Culicoides -borne diseases. In this work, we propose a two-stage method for automatically analyzing Culicoides (Diptera: Ceratopogonidae) species. First, an image preprocessing task composed of median and Wiener filters followed by equalization and morphological operations is used to improve the quality of the wing image in order to allow an adequate segmentation of particles of interest. Then, the segmentation of the zones of interest inside the biting midge wing is made using the watershed transform. The proposed method is able to produce optimal feature vectors that help to identify Culicoides species. A database containing wing images of C. obsoletus , C. pusillus , C. foxi , and C. insignis species was used to test its performance. Feature relevance analysis indicated that the mean of hydraulic radius and eccentricity were relevant for the decision boundary between C. obsoletus and C. pusillus species. In contrast, the number of particles and the mean of the hydraulic radius was relevant for deciding between C. foxi and C. insignis species. Meanwhile, for distinguishing among the four species, the number of particles and zones, and the mean of circularity were the most relevant features. The linear discriminant analysis classifier was the best model for the three experimental classification scenarios previously described, achieving averaged areas under the receiver operating characteristic curve of 0.98, 0.90, and 0.96, respectively.

show abstract

“…Delineation of cryptic species and rapid identification of morphologically similar species are now feasible using genetic markers such as the nuclear ribosomal RNA (rRNA) genes (e.g., [ 6 – 9 ], but see [ 10 ] for caution relating to concerted evolution and the use of rRNA as a genetic marker) and the mitochondrial cytochrome oxidase subunit I (COI) [ 4 , 11 – 14 ] and cytochrome b (Cyt b ) [ 15 ] genes. Important population genetics features, such as migration, gene flow, and mating behaviour (e.g., [ 16 – 18 ]), all benefit from the use of nuclear DNA (nuDNA) and mitochondrial DNA (mtDNA) (e.g., [ 19 – 22 ]), and the use of mtDNA to delineate, and infer the phylogeny of, closely related species has been augmented by using nuDNA markers and morphological data (e.g., [ 14 , 20 , 23 ]).…”

Section: Introductionmentioning

confidence: 99%

“…Globally, there are currently 1401 recognized species (1355 extant species, 46 fossil species) and 31 subgenera of Culicoides [ 24 – 26 ], and many of them are thought to be cryptic species groups. Traditionally, species identification of Culicoides has relied on external (e.g., head or wing patterns [ 12 , 14 ]) and internal (e.g., genitalia [ 27 , 28 ]) traits. Using external traits to identify species can lead to confusion and hamper the identification and confirmation of virus vector status and competency (e.g., C .…”

Section: Introductionmentioning

confidence: 99%

Population Genetic Structure and Potential Incursion Pathways of the Bluetongue Virus Vector Culicoides brevitarsis (Diptera: Ceratopogonidae) in Australia

2016

View full text Add to dashboard Cite

Culicoides brevitarsis is a vector of the bluetongue virus (BTV), which infects sheep and cattle. It is an invasive species in Australia with an assumed Asian/South East Asian origin. Using one mitochondrial marker (i.e., part of the cytochrome oxidase subunit I gene) and six nuclear markers, we inferred population genetic structure and possible incursion pathways for Australian C. brevitarsis. Nine mitochondrial haplotypes, with low nucleotide sequence diversity (0.0–0.7%) among these, were identified in a sample of 70 individuals from seven sites. Both sets of markers revealed a homogeneous population structure, albeit with evidence of isolation by distance and two genetically distinct clusters distributed along a north-to-south cline. No evidence of a cryptic species complex was found. The geographical distribution of the mitochondrial haplotypes is consistent with at least two incursion pathways into Australia since the arrival of suitable livestock hosts. By contrast, 15 mitochondrial haplotypes, with up to four times greater nucleotide sequence diversity (0.0–2.9%) among these, were identified in a sample of 16 individuals of the endemic C. marksi (sampled from a site in South Australia and another in New South Wales). A phylogenetic tree inferred using the mitochondrial marker revealed that the Australian and Japanese samples of C. brevitarsis are as evolutionarily different from one another as some of the other Australian species (e.g., C. marksi, C. henryi, C. pallidothorax) are. The phylogenetic tree placed four of the species endemic to Australia (C. pallidothorax, C. bundyensis, C. marksi, C. henryi) in a clade, with a fifth such species (C. bunrooensis) sharing a common ancestor with that clade and a clade comprising two Japanese species (C. verbosus, C. kibunensis).

show abstract

Taxonomic assessment of Culicoides brunnicans , C. santonicus and C. vexans (Diptera: Ceratopogonidae) in France: Implications in systematics

Cited by 11 publications

References 67 publications

Molecular phylogeny of 42 species ofCulicoides(Diptera, Ceratopogonidae) from three continents

Molecular phylogeny of 42 species ofCulicoides(Diptera, Ceratopogonidae) from three continents

An approach to automatic classification of Culicoides species by learning the wing morphology

Population Genetic Structure and Potential Incursion Pathways of the Bluetongue Virus Vector Culicoides brevitarsis (Diptera: Ceratopogonidae) in Australia

Contact Info

Product

Resources

About