Taxonomic and molecular study on some Asian cultivars of Triticum aestivum L.

Ibrahim, M. M.; Mohamed, Alsafa Hassan; Teleb, Samir; Ibrahim, Sherif; Tantawy, M.

doi:10.21608/taec.2017.11932

Cited by 5 publications

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“…Sonalika from India. The abundance of the ISSR and SCoT markers polymorphism and significant sequence variation support the use of these molecular markers extensively for DNA fingerprinting as a useful tool and straight-forward techniques in genetic diversity studies [ 22 , 29 , 46 ] and confirm that the characterization based on DNA polymorphism, i.e., molecular markers basis is more efficient, accurate, and justifying their wide use in genetic diversity assessment in the last two decades.…”

Section: Discussionmentioning

confidence: 94%

“…The SCoT markers were also employed by Mohamed et al [ 28 ] to genetically characterize 14 cultivars of wheat from North Africa. Also, SCoT was used to discriminate eight wheat Asian cultivars [ 29 ]. Genetic diversity and population structure of 80 Triticum urartu accessions were investigated using SCoT and CBDP markers [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…Knowledge about the genetic diversity of 12 bread wheat cultivars is limited, not only among Northern African varieties but also in cultivars in different countries. The importance of selecting the prementioned wheat cultivars lies in their capability for adaptation to the extreme climatic conditions in the countries where these cultivars were developed [ 28 , 29 ]. In the same context, several pilot and preliminary measurements, including morphological characteristics (ca.…”

Section: Introductionmentioning

confidence: 99%

“…), and grain weight (ca. the weight of one and 20 grains) (data not shown) were performed on previosuly published wheat cultivars [ 28 , 29 ] and revealed focusing on the 12 selected studied cultivars. Also, selected genotypes harbor abiotic resistant genes beside other genes encode transcription factors play a pivotal role in plant growth and development [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

et al. 2023

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Background Wheat is a major cereal that can narrow the gap between the increasing human population and food production. In this connection, assessing genetic diversity and conserving wheat genetic resources for future exploitation is very important for breeding new cultivars that may withstand the expected climate change. The current study evaluates the genetic diversity in selected wheat cultivars using ISSR and SCoT markers, the rbcL and matK chloroplast DNA barcoding, and grain surface sculpture characteristics. We anticipate that these objectives may prioritize using the selected cultivars to improve wheat production. The selected collection of cultivars may lead to the identification of cultivars adapted to a broad spectrum of climatic environments. Results Multivariate clustering analyses of the ISSR and SCoT DNA fingerprinting polymorphism grouped three Egyptian cultivars with cultivar El-Nielain from Sudan, cultivar Aguilal from Morocco, and cultivar Attila from Mexico. In the other group, cultivar Cook from Australia and cultivar Chinese-166 were differentiated from four other cultivars: cultivar Cham-10 from Syria, cultivar Seri-82 from Mexico, cultivar Inqalab-91 from Pakistan, and cultivar Sonalika from India. In the PCA analysis, the Egyptian cultivars were distinct from the other studied cultivars. The rbcL and matK sequence variation analysis indicated similarities between Egyptian cultivars and cultivar Cham-10 from Syria and cultivar Inqalab-91 from Pakistan, whereas cultivar Attila from Mexico was distinguished from all other cultivars. Combining the data of ISSR and SCoT with the rbcL and matK results retained the close resemblance among the two Egyptian cultivars EGY1: Gemmeiza-9 and EGY3: Sakha-93, and the Moroccan cultivar Aguilal, and the Sudanese cultivar El-Nielain and between Seri-82, Inqalab-91, and Sonalika cultivars. The analysis of all data distinguished cultivar Cham-10 from Syria from all other cultivars, and the analysis of grain traits indicated a close resemblance between cv. Cham-10 from and the two Egyptian cultivars Gemmeiza-9 and Sakha-93. Conclusions The analysis of rbcL and matK chloroplast DNA barcoding agrees with the ISSR and the SCoT markers in supporting the close resemblance between the Egyptian cultivars, particularly Gemmeiza-9 and Sakha-93. The ISSR and SCoT data analyses significantly expressed high differentiation levels among the examined cultivars. Cultivars with closer resemblance may be recommended for breeding new wheat cultivars adapted to various climatic environments.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

et al. 2023

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“…The sequences of DNA-SCoT primers were synthesized by Macrogen (Seoul, Republic of Korea) (Table 2). Polymerase chain reaction was performed according to Ibrahim et al (2017). A 1.5% ethidium bromidestained polyacrylamide gel was used to visualize PCR amplicons in 1X TBE buffer.…”

Section: Scot Polymorphismmentioning

confidence: 99%

Genetic diversity analysis reveals weak population structure in invasive Trianthema portulacastrum L. at Fayoum depression, Egypt

2021

Turk J Bot

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Trianthema portulacastrum L. (Aizoaceae) is a common weed associated with cultivated crops. It is an exotic weed that originated in South Africa and is spreading all over the world. Thirty-five accessions were collected from six populations at Fayoum depression (FD), Egypt. Molecular analyses of Start Codon Targeted (SCoT) markers were performed to identify genotypic variation among collected populations. The effectiveness of employing SCoT markers was demonstrated by the high percentage of polymorphisms. These markers revealed high genetic diversity, as well as high levels of genetic differentiation (GST), elevated gene flow (Nm) (0.195 and 2.052, respectively), high variation among a population and lower variation within populations. Linkage disequilibrium analysis supported the presence of sexual and clonal reproduction of T. portulacastrum in different populations. The data confirmed the weak population structure of T. portulacastrum demonstrated in this study via different tools such as STRUCTURE, Minimum spanning network (MSN), and discriminant analysis of principal components (DAPC) and confirmed gene flow between populations. Based on our results, we hypothesize that FD was invaded multiple times by T. portulacastrum facilitated by both local adaptation and phenotypic plasticity.

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Applicability of Start Codon Targeted (SCoT) markers for the assessment of genetic diversity in bread wheat germplasm

Altaf,

Nadeem,

Ali

et al. 2024

Genet Resour Crop Evol

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Research endeavors focused on examining genetic diversity play a pivotal role in advancing agricultural practices, as they furnish valuable insights for breeding programs and the preservation of germplasm. Wheat holds significant global importance as a cereal crop, serving as a primary source of sustenance for almost one-third of the global human population. In the current study, a total of 80 bread wheat genotypes were characterized using the start codon targeted (SCoT) marker system. A set of 14 polymorphic primers yielded a cumulative count of 399 bands with an average of 28.05 bands per primer. The range of diversity markers, such as polymorphism information content (0.26–0.59), effective numbers of alleles (1.27–1.59), Shannon’s information index (0.30–0.51), and gene diversity (0.18–0.34), provided evidence of elevated genetic differences within the examined germplasm. According to the findings of the molecular variance analysis, greater genetic variations are found within populations (92%). The model-based structural approach partitioned the 80 bread wheat genotypes into two distinct populations (Population A and B). The diversity indices based on the structures of the populations revealed that population A had greater diversity compared to population B. Both the principal coordinate analysis and the neighbor-joining analysis also differentiated the 80 different bread wheat genotypes based on their respective collection points. The present crop diversity offers great opportunities for enhancing crop quality and increasing tolerance to environmental challenges, both of which will lead to a reduction in the problem of food insecurity.

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Taxonomic and molecular study on some Asian cultivars of Triticum aestivum L.

Cited by 5 publications

References 0 publications

Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

Genetic diversity analysis reveals weak population structure in invasive Trianthema portulacastrum L. at Fayoum depression, Egypt

Applicability of Start Codon Targeted (SCoT) markers for the assessment of genetic diversity in bread wheat germplasm

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