Taxol purification with Centrifugal Partition Chromatography

Rutterschmidt, D; Kovács, Z.; Lorántfy, László; Misek, Z; Rajsch, G

doi:10.1055/s-0035-1565776

Cited by 2 publications

(1 citation statement)

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“…As mentioned, CPC is capable of realizing complex separation tasks-for example, when it comes to the isolation of 10-Deacetyl Baccatin III, an intermediate in the semisynthetic production of the chemotherapy drug taxol, or the separation of cannabinoids from Cannabis sativa L extract [24,25]. An optimal chromatographic resolution is crucial for those separations, leading to baseline-separated peaks and, therefore, high yield and purity of the target compound.…”

Section: Introductionmentioning

confidence: 99%

Spatially and Temporally Resolved Analysis of Bleeding in a Centrifugal Partition Chromatography Rotor

Buthmann,

Laby,

Hamza

et al. 2024

Separations

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Centrifugal Partition Chromatography (CPC) is a separation technique that utilizes immiscible liquid phases to purify compounds. The selection of solvents in Liquid–Liquid Chromatography offers flexibility and optimization possibilities for specific separation tasks. Understanding the hydrodynamics inside the apparatus is crucial for optimizing a CPC process. The phase retention ratio (Sf) determines the apparatus’s operating point and separation efficiency. However, stationary phase leakage, known as bleeding, complicates the immobilization of this phase. We used a partly transparent single-disc rotor to investigate the time and space dependency of bleeding inside a CPC apparatus, enabling real-time and localized determination of the phase retention ratio. By tracking the retention values over time, we observed the bleeding phenomenon and its progression from the inlet to the rotor outlet. Depending on the phase system used, the CPC was utilizable for a separation task for only 173–500 dimensionless residence times. Systems with a higher stability parameter (as described in the literature) showed a lower bleeding rate and increased stability over time. Accordingly, our results demonstrate the importance of maintaining an optimal ratio of mobile to stationary phase for efficient separation.

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Section: Introductionmentioning

confidence: 99%