Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cells

Chen, Tongsheng; Wang, Xiaoping; Sun, Lei; Wang, Longxiang; Xing, Da; Mok, Martin S.

doi:10.1016/j.canlet.2008.05.008

Cited by 54 publications

(58 citation statements)

References 25 publications

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“…EGFP was excited at 488 nm and°uorescence emission was recorded through a 500-550 nm band-pass¯lter. [12][13][14] In this study, 70 M of taxol was adopted in all experiments as described previously. 12,13 We used FCM analysis to assess the temporal relationship between the taxol-induced cytoplasm vacuolization and dissipation of ÁÉm.…”

Section: Monitoring Microtubule Distribution Inside Living Cells By Cmentioning

confidence: 99%

“…12,13 In order to further determine whether taxol induces cell death in apoptotic fashion, we used FCM to analyze the externalization of PS and membrane integrity of cells co-stained by Annexin V/PI. As shown in Fig.…”

Section: Taxol Induces Nonapoptotic Cell Deathmentioning

confidence: 99%

“…4 Because the mitochondrial generation of ATP requires electrochemical gradient, a reduction in mitochondrial membrane potential may precede the morphologic changes observed in oncotic cell death. 11 We recently found that high concentration of taxol (70 M) induced cell death with cytoplasm vaculization in paraptosis-like fashion in HeLa, U87, A549 and ASTC-a-1 cell lines, [12][13][14] and low concentration of taxol (35 nM) induced cell death mainly in apoptotic fashion. 15 Cytoplasm vacuolization from endoplasmic reticulum (ER) and/or mitochondrial swelling together with absence of apoptotic DNA fragmentation and caspase activation are the characteristics of paraptosis.…”

Section: Introductionmentioning

confidence: 97%

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TAXOL INDUCES CELL DEATH WITH CYTOPLASM VACUOLIZATION IN PARAPTOSIS-LIKE BUT NOT ONCOSIS FASHION IN ASTC-a-1 CELLS

Quan

Wang

et al. 2013

J. Innov. Opt. Health Sci.

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Recently, we found that high concentration of taxol (70 M) induced cell death with cytoplasm vacuolization, the typical characteristic of both paraptosis and oncosis, in human lung carcinoma (ASTC-a-1) cells. This report was designed to further determine the form of taxol-induced cell death with cytoplasm vacuolization. It is generally considered that the cytoplasm vacuolization in oncosis due to the swelling of endoplasmic reticulum (ER), mitochondria, lysosomes and nuclei occurs after the loss of mitochondrial membrane potential (ÁÉm). However,°ow cytometry (FCM) analysis showed that taxol-induced cytoplasm vacuolization preceded the loss of ÁÉm. Moreover, taxol treatment did not induce the collapse of microtubule, the typical characteristic of oncosis. These data demonstrated that taxol-induced cell death with cytoplasm vacuolization is not oncosis. FCM analysis by Annexin V-FITC/PI apoptosis detection kit further demonstrated that taxol-induced cell death with cytoplasm vacuolization is not apoptosis. In conclusion, in combination with our recent in vitro and in vivo data, this report further demonstrates that high concentration of taxol induces cell death with cytoplasm vacuolization in paraptosis-like but not oncosis fashion.

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Section: Monitoring Microtubule Distribution Inside Living Cells By Cmentioning

confidence: 99%

Section: Taxol Induces Nonapoptotic Cell Deathmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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TAXOL INDUCES CELL DEATH WITH CYTOPLASM VACUOLIZATION IN PARAPTOSIS-LIKE BUT NOT ONCOSIS FASHION IN ASTC-a-1 CELLS

Quan

Wang

et al. 2013

J. Innov. Opt. Health Sci.

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“…By ER probe erRFP plasmid and caspase-3 probe SCAT3 plasmid, Chen et al [56] found that taxol induced a paraptosislike programmed cell death in human lung adenocarcinoma (ASTC-a-1) cells by cytoplasm vacuolization due to the swelling of both ER and mitochondria without activating the caspase enzymes. Latex beads are the preferred phagocytic substrate in biochemical studies of phagosome composition and maturation.…”

Section: Organellesmentioning

confidence: 99%

Application of Probes in Live Cell Imaging~!2010-01-03~!2010-05-09~!2010-08-16~!

Chen¹,

Bai²,

Wang³

2010

JEBP

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Live cell imaging (LCI) is the approach of non-invasively analyzing dynamic processes in living cells using state-of-the-art microscopy and computer vision techniques. LCI provides exciting and novel insights into cell biology. The present review summarized LCI research in recent years and detailed the role of probes in LCI. We discussed the basic principle and development in LCI technology, including fluorecence resonance energy transfer, fluorescence lifetime imaging microscopy, atomic force microscopy, scanning ion conductance microscopy, confocal microscopy, two-photon excitation fluorescence microscopy and Cell-IQ® System . Then we listed several probes such as green fluorescent protein and quantum dots which are the most widely used probes. Proteins, organelles, nucleotides and other chemicals related to cell biology may all be potential targets. We also discussed the advantages and disadvantages of different probes. Although specific probes in LCI still need to be explored, the application of LCI may be attractive for better understanding of cellular functions.

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“…This agent is the first member of the taxel family (17) and is effective in treating certain types of cancer (18). Paclitaxel has been shown to stabilize microtube polymers, leading to mitotic arrest and apoptosis (19).…”

Section: Sensitization Of Mda-mb-231 Cells To Paclitaxel By Inhibitiomentioning

confidence: 99%

Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells

Wang²,

Zeng³

et al. 2010

Oncol Rep

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Abstract. Rab is a large subfamily of small GTPases which play an important role in multiple processes relating to cellular transportation and modulation of the cytoskeleton. Novel gene Rab-like 3 (Rabl3), which we originally reported as a new member belonging to this subfamily, may participate in other processes in human cancer cell lines based on our research. In order to investigate the function of Rabl3 and the basic mechanism which regulates cancer cell survival and motility, we constructed the Rabl3-pcDNA3.1 fusion plasmid, also, the specific siRNA against Rabl3 was used. We detected cellular viability and motility changes in Rabl3 overexpressed or si-Rabl3 transfected cancer cell lines. Overexpression of Rabl3 resulted in the enhancement of cell proliferation, inhibition of apoptosis and paclitaxel resistance in human cancer cell lines. Rabl3 also promoted cell motility activity. Next, we silenced Rabl3 in order to determine its exact physiological function. We found that processes which are associated with tumor formation and metastasis were inhibited. These included an increased incidence of apoptosis, abrogated cellular proliferation and mobility. Furthermore, Western blot analysis revealed that the function of Rabl3 was closely associated with focal adhesion kinase (FAK) phosphorylation, both in HeLa and MDA-MB-231 cell lines at the sites of Tyr 397/576/577. Our results suggested that Rabl3 may be a novel oncogene which regulates the oncological behavior of human cancer cells. As a consequence, Rabl3 can be considered as a novel candidate in the control of tumorigenesis and as a new target for anti-tumor treatment.

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Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cells

Cited by 54 publications

References 25 publications

TAXOL INDUCES CELL DEATH WITH CYTOPLASM VACUOLIZATION IN PARAPTOSIS-LIKE BUT NOT ONCOSIS FASHION IN ASTC-a-1 CELLS

TAXOL INDUCES CELL DEATH WITH CYTOPLASM VACUOLIZATION IN PARAPTOSIS-LIKE BUT NOT ONCOSIS FASHION IN ASTC-a-1 CELLS

Application of Probes in Live Cell Imaging~!2010-01-03~!2010-05-09~!2010-08-16~!

Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells

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