Taxogenomic and Metabolic Insights into Marinobacterium ramblicola sp. nov., a New Slightly Halophilic Bacterium Isolated from Rambla Salada, Murcia

Abstract

A Gram-negative, motile, rod-shaped bacteria, designated D7T, was isolated by using the dilution-to-extinction method, from a soil sample taken from Rambla Salada (Murcia, Spain). Growth of strain D7T was observed at 15–40 °C (optimum, 37 °C), pH 5–9 (optimum, 7) and 0–7.5% (w/v) NaCl (optimum, 3%). It is facultatively anaerobic. Phylogenetic analysis based on 16S rRNA gene sequence showed it belongs to the genus Marinobacterium. The in silico DDH and ANI against closest Marinobacterium relatives support its placement as a new species within this genus. The major fatty acids of strain D7T were C16:0, summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol and two uncharacterized lipids. Ubiquinone 8 was the unique isoprenoid quinone detected. The DNA G + C content was 59.2 mol%. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genomic characterization, strain D7T (= CECT 9818T = LMG 31312T) represents a novel species of the genus Marinobacterium for which the name Marinobacterium ramblicola sp. nov. is proposed. Genome-based metabolic reconstructions of strain D7T suggested a heterotrophic and chemolitotrophic lifestyle, as well as the capacity to biosynthetize and catabolize compatible solutes, and to degrade hydrocarbon aromatic compounds.

Bacterial diversity of smoked and smoked‐dried fish from West Africa: A metagenomic approach

“…Important inter-individual variation in the composition of bacterial communities among fishes of the same species has also been previously reported (Arias et al, 2019;Boutin et al, 2014). The detection of the genus Photobacterium in sea fish with relative abundance higher than in lake fish, and the presence of Salinicoccus, Marinobacter, and Marinobacterium genera exclusively in sea fish is likely due to the fact that these bacteria are halophilic, as previously reported (Chen et al, 2007;Durán-Viseras et al, 2021;Grimaud, 2010).…”

Evidence for Assimilatory Nitrate Reduction as a Previously Overlooked Pathway of Reactive Nitrogen Transformation in Estuarine Suspended Particulate Matter

“…In contrast, only two high-quality nirBD -carrying MAGs (i.e., MAG nos. 39 and 57) from Marinobacterium and Planctomycetaceae were likely the fermentative DNRA bacteria as they lacked most of the catalytic and regulatory genes related to ANR. , …”

“…39 and 57) from Marinobacterium and Planctomycetaceae were likely the fermentative DNRA bacteria as they lacked most of the catalytic and regulatory genes related to ANR. 79,80 The genomic loci within each of 37 nirBD-carrying MAGs were screened for nirBD and nasA in the same gene cluster as this is a genetic-level prerequisite for ANR. 73,74 Data revealed that 24 of 37 nirBD-carrying MAGs possessed a gene cluster containing nirBD and nasA together (Figure S2).…”

Special Issue “Halophilic Microorganisms”

“…Both classical culture techniques and metagenomic techniques have been used. The four research papers in this Special Issue are related to the study of biodiversity in different hypersaline environments [ 5 , 6 , 7 , 8 ].…”