Taxifolin and Fucoidin Abolish the Irradiation-Induced Increase in the Production of Reactive Oxygen Species in Rat Aorta

Arutyunyan, T. V.; Korystova, A. F.; Kublik, L. N.; Levitman, M. Kh.; Shaposhnikova, V. V.; Korystov, Yu. N.

doi:10.1007/s10517-016-3236-2

Cited by 15 publications

(14 citation statements)

References 11 publications

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“…The absorbance was measured at 420 nm. Glutathione peroxidase (Gpx) activity was determined in tissue homogenate by measuring the absorbance at 340 nm (Arutyunyan et al 2016; Jordão et al 2004; Erden-Inal et al 2002).…”

Section: Methodsmentioning

confidence: 99%

Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis

Hu¹,

Peng³

2019

AMB Expr

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The aim of the present study was to evaluate the protective effects of combined atorvastatin and amygdalin in a rat model of endometriosis. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), matrix metalloproteinase-2 (MMP-2) and MMP-9 levels in the peritoneal fluid were determined. The expression of TNF-α, IL-6, MMP-2, and MMP-9 mRNA, and the levels of lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD), catalase, and glutathione peroxidase (Gpx) were measured. Histopathological analysis was also conducted. The results showed that peritoneal TNF-α, IL-6, MMP-2, and MMP-9 levels were reduced by > 50%, and mRNA expression was decreased. Lipid peroxidation was considerably reduced, while GSH, SOD, Gpx, and catalase levels increased by > 40%. Reductions in leukocyte infiltration and fibrosis following treatment were also observed. Thus, our study suggested that combined treatment consisting of atorvastatin and amygdalin attenuates endometriosis. A detailed investigation of molecular mechanism of atorvastatin and amygdalin in endometriosis is needed.

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Section: Methodsmentioning

confidence: 99%

Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis

Hu¹,

Peng³

2019

AMB Expr

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“…Quercetin has been demonstrated to improve post ischemic recovery of the isolated heart of rats after global ischemia (Barteková et al, 2010). Taxifolin (TAX) exerts anti-inflammatory effects and prevents oxidative stress-induced injury in human endothelial cells (Guo et al, 2015) and rat peritoneal macrophages (Arutyunyan et al, 2016). It also possesses free radical scavenging, antioxidant and anti-inflammatory effects (Sun et al, 2014; Xie et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway

Tang

Yang

Zuo

et al. 2019

PeerJ

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BackgroundTaxifolin (TAX), is an active flavonoid, that plays an underlying protective role on the cardiovascular system. This study aimed to evaluate its effect and potential mechanisms on myocardial ischemia/reperfusion (I/R) injury.MethodsHealthy rat heart was subjected to I/R using the Langendorff apparatus. Hemodynamic parameters, including heart rate, left ventricular developed pressure (LVDP), maximum/minimum rate of the left ventricular pressure rise (+dp/dtmax and −dp/dtmin) and rate pressure product (RPP) were recorded during the perfusion. Histopathological examination of left ventricular was measured by hematoxylin-eosin (H&E) staining. Creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) activities in the effluent perfusion, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) in the tissue were assayed. Apoptosis related proteins, such as B-cell lymphoma-2 (Bcl-2), Bcl2-associated X (Bax), and cytochrome c (Cyt-c) were also assayed by ELISA. Western blot was employed to determine apoptosis-executive proteins, including caspase 3 and 9. Transferase-mediated dUTP-X nick end labeling assay was performed to evaluate the effect TAX on myocardial apoptosis.ResultsTaxifolin significantly improved the ventricular functional recovery, as evident by the increase in LVDP, +dp/dtmax, −dp/dtmin and RPP, the levels of SOD, GSH-PX were also increased, but those of LDH, CK-MB, and MDA were decreased. Furthermore, TAX up-regulated the Bcl-2 protein level but down-regulated the levels of Bax, Cyt-c, caspase 3 and 9 protein, thereby inhibits the myocardial apoptosis.DiscussionTaxifolin treatment remarkably improved the cardiac function, regulated oxidative stress and attenuated apoptosis. Hence, TAX has a cardioprotective effect against I/R injury by modulating mitochondrial apoptosis pathway.

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“…Lipid peroxidation was determined according to malondialdehyde (MDA) content (Jordão et al 2004 ). Reactive oxygen species (ROS) were determined using the dichlorofluorescein assay (Arutyunyan et al 2016 ). The reduced glutathione (GSH) level was measured by determining the final product at 405 nm (ErdenInal et al 2003 ).…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory and anxiolytic activities of Euphorbia hirta extract in neonatal asthmatic rats

et al. 2018

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The current study evaluated the anti-inflammatory and anxiolytic activities of Euphorbia hirta extract in neonatal asthmatic rats. Rats were assigned to the following groups: group I, sham (normal rats); group II, control (asthmatic rats); group III, E. hirta extract (100 µg/100 µl) and group IV, E. hirta extract (200 µg/100 µl). We performed a phytoscreening analysis of E. hirta extract. Inflammatory cell counts in the bronchoalveolar lavage fluid, levels of anti-inflammatory and antioxidant markers, apoptosis, and a histopathological analysis were carried out. An open field test determined anxiolytic activity, an elevated plus maze, a hole board test, and a cross test. The presence of 9,12,15-octadecatrien-1-ol, pentadecylic acid, ethyl linoleate, 1,2,3-trihydroxy benzene, gamma-tocopherol, 5-hydroxymethyl-2-furancarboxaldehyde, myristic acid, 7,10-octadecadienoic acid methyl ester, phytol, ethyl palmitate, and squalene in E. hirta extract was noted. Following treatment with E. hirta extract, total leukocytes, eosinophils, tumor necrosis factor-α (TNF-α), interleukin (IL-6), and lipid peroxidation were reduced, whereas antioxidant levels were increased. The mRNA expression levels of TNF-α, inducible nitric oxide synthase, IL-6, cyclooxygenase-2, caspase-3, p53, nerve growth factor precursor, and Bax were reduced, whereas that of Bcl-2 was increased. Apoptosis and caspase-3 protein expression were significantly reduced. Treatment of rats with E. hirta extract significantly reduced inflammation and eosinophil infiltration in the lungs. Taken together, these results led us to conclude that E. hirta extract has anti-inflammatory and anxiolytic effects on neonatal asthmatic rats with inflammation.

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Taxifolin and Fucoidin Abolish the Irradiation-Induced Increase in the Production of Reactive Oxygen Species in Rat Aorta

Cited by 15 publications

References 11 publications

Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis

Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis

Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway

Anti-inflammatory and anxiolytic activities of Euphorbia hirta extract in neonatal asthmatic rats

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