Taxifolin ameliorates DEHP-induced cardiomyocyte hypertrophy via attenuating mitochondrial dysfunction and glycometabolism disorder in chicken

Cai, Jingzeng; Shi, Guangliang; Zhang, Yuan; Zheng, Yingying; Yang, Jie; Liu, Qi; Gong, Yafan; Yu, Dahai

doi:10.1016/j.envpol.2019.113155

Cited by 46 publications

(15 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…cDNA was synthesized from 5 μg of the total RNA using oligo dT primers and Superscript II reverse transcriptase according to the manufacturer's instructions (Promega). cDNA was diluted at a ratio of 1:5 with sterile water and stored at −80°C (Cai et al, ).…”

Section: Methodsmentioning

confidence: 99%

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Yang

Gong

Cai

et al. 2020

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

Chlorpyrifos is a poisonous pesticide that is highly toxic to fish and aquatic organisms. However, there are fewer reports about how chlorpyrifos influences the redox balance of immune cells. Herein, the head kidney tissue treated with chlorpyrifos to do transcriptome analysis and TCR γ was screened out. Subsequently, we established TCR γ knockdown and overexpression carp head kidney lymphocyte models, respectively, by using RNA interference and pcDNA3.1. Real‐time PCR, fluorescent staining, oxidation and antioxidant kit were used to detect the related factors. We found that TCR γ knockdown significantly increased the mRNA expression of HSP70 and HSP90 and decreased the mRNA expression of SOD and CAT. Meanwhile, TCR γ knockdown reduced the activities of GSH, GSG‐PX, T‐AOC, CAT and SOD and increased the content of MDA and H2O2 and activities of iNOS. Adverse results were obtained in TCR γ overexpression group. Additionally, TCR γ knockdown significantly increased the mRNA expression of IFN‐γ, IL‐1β, IL‐8, IL‐10, Nrf2 and NF‐κB, but relieved TCR γ overexpression, in which the process of inflammation was activated. Our results reported here indicated that chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ, and HSPs play potential protective role in entire process.

show abstract

Section: Methodsmentioning

confidence: 99%

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Yang

Gong

Cai

et al. 2020

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous reports have shown that MOL000354 (isorhamnetin) can exhibit a positive effect on injuring cardiomyocytes by upregulation of SIRT1 and Nrf2/HO-1-mediated antioxidant signaling pathway, protective targeting cardiac hypertrophy by blocking the phosphatidylinositol 3-kinase-AKT signaling pathway [38,39]. Furthermore, MOL004576 (taxifolin), a plant avonoid separated from yew, can alleviate cardiac hypertrophy by activating the IL-6/JAK/STAT3 signal pathway, triggering glycometabolism disorder and mitochondrial dysfunction in vitro [40], and also remarkably improve the cardiac function, regulated oxidative stress and attenuated apoptosis pathway [41]. However, there was no literature relating to MOL000380, MOL000371, MOL002392, MOL000049, and MOL001735 with DCM.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Pharmacological Mechanism of the modified Chinese medicine formulas “Shenfu-Linguizhugan decoction” treating Dilated cardiomyopathy Based on Network Pharmacology

Quan¹,

Miao²

2020

Preprint

View full text Add to dashboard Cite

Background: Dilated cardiomyopathy (DCM) is a non-ischaemic cardiac muscle disease with structural and functional myocardial aberration can lead to extensive morbidity and mortality due to complications in particular heart failure and arrhythmia. Two classic Chinese medicine formulas, Shenfu decoction and Linguizhugan decoction, were both shown to exert therapeutic effects on heart disease. Thus, modified Shenfu and Linguizhugan decoction (SFLGZGD) is recommended for treatment DCM. However, its chemical and pharmacological characteristics remain to be elucidated. In the current study, a network pharmacology approach was applied to characterize the action mechanism and target genes of SFLGZGD on DCM.Methods: The gene expression of DCM was obtained from the Gene Expression Omnibus (GEO). All compounds were obtained from the correlative databases, and active mixture were selected according to their oral bioavailability (OB) and drug-likeness (DL) index. The potential targets of SFLGZGD were obtained from the traditional Chinese medicine systems pharmacology (TCMSP) database. The compound-target and target-pathway networks were constructed. The protein-protein interactive (PPI) network generated by R software was visualized by Cytoscape, and the topology scores, functional regions, and gene annotations were analyzed using plugins of Bisogenet and CytoNCA. The potential pathways related to target genes were determined by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.Results: A total of 963 differentially expressed genes (DEGs), including 538 upregulated genes and 425 downregulated, were obtained from GSE19303. A total of 636 ingredients in SFLGZGD were obtained, among which, 93 were chosen as bioactive components. The compound-target network included 10 bioactive components and 18 potential targets and a total of 1939 genes obtained in the PPI network, among them, a total of 16 genes were screened out. Moreover,129 terms on the GO analysis and six pathways obtained. Among these potential targets, EGFR, CDKN1A, MMP1, COL1A1, COL3A1, MMP3, ICAM1, and HSPB1 were identified as relatively high-degree targets.Conclusions: The network pharmacology-based approach in the current study has shown promising potential in identifying major therapeutic targets from TCM formulations. Besides, our study suggested that network pharmacology prediction may provide a useful tool for describing the molecular mechanism of SFLGZGD on DCM.

show abstract

“…DEHP exposure in male quail results in increased cardiomyocyte swelling and muscle fiber dilation ( Wang et al, 2019 ). Phthalate treatment of chicken cardiomyocytes induces cardiomyocyte hypertrophy via mitochondrial dysfunction ( Cai et al, 2019 ). As in testing to assess BPA toxicity, developmental exposure of zebrafish to phthalates induces cardiac morphological abnormalities, pericardial edema, and reduced cardiac function ( Pu et al, 2020 ; Sun and Li, 2019 ; Sun and Liu, 2017 ).…”

Section: Case Study 2: Cardiovascular Disease (Cvd) and Non-eats Moda...mentioning

confidence: 99%

Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities

Martyniuk

Martínez

Navarro‐Martín

et al. 2022

Environmental Research

View full text Add to dashboard Cite

Taxifolin ameliorates DEHP-induced cardiomyocyte hypertrophy via attenuating mitochondrial dysfunction and glycometabolism disorder in chicken

Cited by 46 publications

References 57 publications

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ

Exploring the Pharmacological Mechanism of the modified Chinese medicine formulas “Shenfu-Linguizhugan decoction” treating Dilated cardiomyopathy Based on Network Pharmacology

Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities

Contact Info

Product

Resources

About